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Preliminary in Vitro Screening for Antioxidant and Anti-MCF7-Breast

Cancer Cell Line Activities of Folk Healersû Tonic Remedies
†
,§
Pariyapat Singthong* , Pornprapha Chunthanom , Sasitorn Chusri ‡
*Faculty of Thai Traditional and Alternative Medicine, Ubonratchathani Rajabhat University, Amphoe Mueang,
Ubon Ratchathani 34000, Thailand
†
Department of Food Science and Technology, Rajamangala University of Technology Isan, Sakon Nakhon campus,
Sakon Nakhon 47160, Thailand
‡
Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand
§
Corresponding author: pariyapat.bs@gmail.com

Abstract

This study aimed to assess the antioxidant and anticancer activities of ethanol extracts of 15 herbal
recipes from Thai indigenous healers at Sakon Nakhon Thai Traditional Medicine Hospital, using the DPPH
radical scavenging and the ferric-reducing antioxidant power (FRAP) assays. In this effort, total phenolic,
flavonoid and tannin contents were quantified; and their anti-cancer as well as cytotoxic properties were
evaluated in vitro against Vero cell and MCF7-breast cancer cell line. Results showed that the percentages of
maximum anti-oxidant activities, using the DPPH assay, of herbal recipe numbers THS013, THS007 and
THS006 were 181.6 ± 0.9, 71.7 ± 3.9 and 69.9 ± 2.0, respectively. The ferric-reducing activities for recipe
numbers THS008, THS015 and THS013 were 3.2 ± 0.0, 3.1 ± 0.1 and 3.0 ± 0.0 mg of ferrous sulphate
equivalence/g of extract, respectively. The highest total phenolic contents of 3.4 mg GAE/ml were found in
THS015 extract; the highest flavonoid contents of 4.1 mg CE/ml in THS014 extract; and the highest tannin
contents of 2.3 mg TAE/ml in THS003 extract. Moreover, the THS001 extract was found to have the highest
anti-Vero cell capacity with an IC of 18.73 μg/ml; and for all herbal extracts, their IC values for anti-MCF
50 50
7-breast cancer cell activities were > 50 μg/ml. Thus, further studies should be conducted on such recipesû
efficacies and safety in detail prior to developing them as health products.

Key words: antioxidant, anticancer, tonic remedies, folk healers

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