Page 18 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 16 ฉบับที่ 3

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·≈â«π”‰ª√–‡À¬·Àâߥ⫬‡§√◊ËÕß Rotary Evaporator “√≈–≈“¬ FRAP 1 ml ∫ࡉ«â 30 π“∑’ Blank ¢Õß

À≈—ß®“°π—Èππ” “√ °—¥¡“§”π«≥À“√âÕ¬≈–¢Õß “√ “√ °—¥ §◊Õ ‡Õ∑“πÕ≈ 95% «—¥§à“¥Ÿ¥°≈◊π· ß∑’Ë
°—¥À¬“∫ (%yield) §«“¡¬“«§≈◊Ëπ 593 nm ¥â«¬‡§√◊ËÕß UV-Vis Spec-

3. °“√∑¥ Õ∫ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √– trophotometer ‚¥¬„™â‡øÕ√— ´—≈‡øμ (ferrous
3.1 °“√∑¥ Õ∫¥â«¬«‘∏’ DPPH radical sulphate) ‡ªìπ “√¡“μ√∞“𠧔π«≥ª√‘¡“≥ “√
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scavenging assay FRAP ∑—ÈßÀ¡¥¢Õß “√ °—¥ ‚¥¬‡ª√’¬∫‡∑’¬∫°—∫
°“√‡μ√’¬¡ “√ °—¥ ™—Ëß “√ °—¥ 0.1 g “√¡“μ√∞“π¢Õ߇øÕ√— ´—≈‡øμ √“¬ß“π§à“„π√Ÿª
≈–≈“¬„π‡Õ∑“πÕ≈ 95% ª√‘¡“≥ 10 ml ‡°Á∫‰«â„π ·∫∫¡‘≈≈‘°√—¡¢Õ߇øÕ√— ´—≈‡øμμàÕ°√—¡¢Õß “√ °—¥

¢«¥ª√—∫ª√‘¡“≥ ‡μ√’¬¡ “√≈–≈“¬ DPPH ‚¥¬™—Ëß “√ (mg of Ferrous Sulphate equivalence/g of extract)
DPPH πÈ”Àπ—° 0.188 g ≈–≈“¬¥â«¬‡Õ∑“πÕ≈ 40% 4. °“√À“ª√‘¡“≥°≈ÿà¡ “√øïπÕ≈‘° ‚¥¬«‘∏’
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ª√‘¡“≥ 100 ml ‡°Á∫‰«â„π¢«¥ ’™“ Folin-Ciocalteu Colorimetric Method
°“√«‘‡§√“–Àå ªî‡ªμ “√ °—¥ª√‘¡“≥ 0.1 ml °“√‡μ√’¬¡ “√ °—¥ ™—Ëß “√ °—¥ 0.1 g ≈–≈“¬
‡Õ∑“πÕ≈ 95% ª√‘¡“≥ 5.9 ml ·≈– “√ DPPH ¥â«¬‡Õ∑“πÕ≈ 95% ª√‘¡“≥ 10 ml ‡μ√’¬¡ “√≈–≈“¬
ª√‘¡“≥ 0.1 ml ≈ß„πÀ≈Õ¥∑¥≈Õß ‡¢¬à“„À⇢⓰—π ™—Ëß‚´‡¥’¬¡§“√å∫Õ‡πμ 6 g ≈–≈“¬·≈–ª√—∫ª√‘¡“μ√

μ—Èß∑‘È߉«â„π∑’Ë¡◊¥‡ªìπ‡«≈“ 30 π“∑’ ·≈–«—¥§à“¥Ÿ¥°≈◊π 100 ml ¥â«¬πÈ”°≈—Ëπ ‡μ√’¬¡ “√¡“μ√∞“π ™—Ëß°√¥
· ß∑’˧«“¡¬“«§≈◊Ëπ 517 nm ¥â«¬‡§√◊ËÕß UV-Vis ·°≈≈‘§ 0.1 g ª√—∫ª√‘¡“μ√¥â«¬πÈ”°≈—Ëπ 100 ml
Spectrophotometer √“¬ß“πº≈‡ªìπ‡ªÕ√凴Áπμå ‡®◊Õ®“ß “√¡“μ√∞“π„Àâ¡’§«“¡‡¢â¡¢âπ 0.1, 0.2, 0.4,

°“√¬—∫¬—Èß (%inhibition) Õπÿ¡Ÿ≈Õ‘ √– DPPH 0.6, 0.8 ·≈– 1 mg/ml
3.2 °“√∑¥ Õ∫¥â«¬«‘∏’ Ferric reducing °“√«‘‡§√“–Àå ªî‡ªμ “√μ—«Õ¬à“ß 200 μl ≈ß„π
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antioxidant power (FRAP) À≈Õ¥∑¥≈Õß ‡μ‘¡ Folin-Ciocalteuûs reagent
°“√‡μ√’¬¡ “√ °—¥ ™—Ëß “√ °—¥ 0.1 g solution 150 μl ‡μ‘¡‡Õ∑“πÕ≈ 95% ª√‘¡“≥ 11.35
≈–≈“¬¥â«¬‡Õ∑“πÕ≈ 95% ª√‘¡“≥ 10 ml °“√‡μ√’¬¡ ml ∑‘È߉«â 5 π“∑’ ·≈â«‡μ‘¡‚´‡¥’¬¡§“√å∫Õπ‡πμ 0.5

“√≈–≈“¬ FRAP ™—Ëß‚´‡¥’¬¡Õ–´’‡μμ∫—ø‡øÕ√å ml ∑‘È߉«â 90 π“∑’ Blank ¢Õß “√ °—¥ §◊Õ ‡Õ∑“πÕ≈
3.1 g ·≈â«‡μ‘¡°√¥Õ–´’μ‘° 16 ml ª√—∫ª√‘¡“≥ 1 L 95% «—¥§à“¥Ÿ¥°≈◊π· ß∑’˧«“¡¬“«§≈◊Ëπ 725 nm

¥â«¬πÈ”°≈—Ëπ ‡μ√’¬¡‰Œ‚¥§≈Õ√‘° 40 mM ™—Ëß “√ ¥â«¬‡§√◊ËÕß UV-Vis Spectrophotometer √“¬ß“π
TPBZ 0.031 g ª√—∫ª√‘¡“≥ 10 ml ¥â«¬‰Œ‚¥√§≈Õ√‘° „π√Ÿª·∫∫¡‘≈≈‘°√—¡¢Õß°√¥·°≈≈‘§μàÕ°√—¡¢Õß “√
≈–≈“¬„π Hot plate 50˚C °“√‡μ√’¬¡ “√¡“μ√∞“π °—¥ (mg of Gallic acid equivalence (GAE)/g

™—Ëß “√≈–≈“¬‡øÕ√— ´—≈‡øμ 0.1 g ª√—∫ª√‘¡“μ√ of extract)
¥â«¬πÈ”°≈—Ëπ 100 ml ®“°π—Èπ‡®◊Õ®“ß “√≈–≈“¬ 5. °“√À“ª√‘¡“≥°≈ÿà¡ “√ø≈“‚«πÕ¬¥å ‚¥¬«‘∏’
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¡“μ√∞“π„Àâ¡’§«“¡‡¢â¡¢âπ 0.1, 0.2, 0.4, 0.6, 0.8 Aluminium Chloride Colorimetry hexahydrate
·≈– 1 mg/ml °“√‡μ√’¬¡ “√ °—¥ ™—Ëß “√ °—¥ 0.1 g ≈–≈“¬
°“√«‘‡§√“–Àå ªî‡ªμ “√ °—¥ 0.5 ml ªî‡ªμ ¥â«¬‡Õ∑“πÕ≈ 95% ª√‘¡“≥ 10 ml ‡μ√’¬¡ “√≈–≈“¬