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J Thai Trad Alt Med Vol. 21 No. 3 Sep-Dec 2023 509
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cal activities in vitro and in vivo need to be The 12 article, Callus Induction and Ros-
conducted for a long time before moving on marinic Acid Production of Helicteres isora
to clinical trials in humans. L., involves the resolution of inconsistency in
The ninth article, Comparison of Cyto- the production of active ingredients of H. isora
toxic Activity against Gastrointestinal Can- plant, or po bit in Thai, by developing a suitable
cer Cell Lines of Basella alba L. and Basella callus culture media formula in the production
rubra L. Extracts, shows that the extract of rosmarinic acid. The amount of the active
from fresh B. rubra (phak plang daeng in Thai) ingredient obtained is 4.5-fold higher than that
with 95% ethanol and further with chloroform obtained from naturally grown po bit and 31-
has the highest cytotoxic activity against fold higher than planted po bit pods. The po
gastrointestinal cancer cell lines. The extract bit tissue culture has a several advantages, i.e.
can be further analyzed to determine active fewer steps than naturally growing the plant,
ingredients for use in lab animal testing and no need for chemical pesticides, less heavy
toxicity testing, and for further development as metal contamination from soil, and less mi-
herbal products. The 10 article, Antibacterial crobial contamination from the use of external
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and Antioxidant Activities of Shallot (Al- raw materials. Thus, it is a method for getting
lium ascalonicum) Extract in Lablae District, a high-quality raw material for producing vari-
Uttaradit Province, suggests that shallot ex- ous products that can effectively enhance the
tract has antioxidant activity and can inhibit economic value.
the microbial growth of Staphylococcus aureus, This issue has two review articles. The
S. epidermidis, and Bacillus cereus. The ex- first article, Herbal Extract Preparation Pro-
tract may be further developed for enhancing cess, is the collection of information from a lot
the value of shallots. The 11 article, Antioxi- of international textbooks and journals. The
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dant and Nitric Oxide Inhibitory Activities information has been written superbly after
of “Ya-Kae-Lom-Utthang-Ka-Ma-Va-Ta’’ Thai having reviewed the matter in all aspects; so
Traditional Medicine Formula in ATDC-5 the article on herbal extract preparation has
Cell, indicates that the extract has antioxidant been presented in a concise and clear manner.
activity and anti-inflammatory activity through It is thus extremely valuable in the field of Thai
the inhibition of nitric oxide production related traditional medicine and medicinal herbs. The
to knee osteoarthritis (KOA). Thus, it can be second article, Selection of Thai Traditional
further developed as a KOA therapy, but many Medicines Formulas for Treatment of Mus-
more relevant studies are to be undertaken. culoskeletal Disorders, is a systemic review of