Page 37 - วารสารปีที่16ฉบับที่2

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·∫∫¢Õ߇À≈« ¡√√∂π– Ÿß (HPLC fingerprint) Õ–´‘‡μ∑¡“·¬°∫√‘ ÿ∑∏‘ϥ⫬§Õ≈—¡πå™π‘¥√’‡«Õ√å ‡ø

‡æ◊ËÕ»÷°…“Õߧåª√–°Õ∫∑“߇§¡’¢Õß “√ °—¥∑Õßæ—π™—Ëß (Grom-Sil 120 ODS-5 ST; 250 × 4 mm, 10 μm)
√«¡ 5 μ—«Õ¬à“ß ‰¥â·°à “√ °—¥‡Õ∑“πÕ≈®“°„∫ ≈”μâπ ∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕß (25 Õß»“‡´≈‡´’¬ ) „™â«—Ø¿“§

√“° ·≈– “√ °—¥‡Õ∑‘≈Õ–´‘‡μ∑®“°„∫·≈–√“° ‡§≈◊ËÕπ∑’˪√–°Õ∫¥â«¬√âÕ¬≈– 0.1 °√¥‰μ√ø≈ŸÕÕ
¥”‡π‘π°“√‡μ√’¬¡μ—«Õ¬à“ß ”À√—∫°“√»÷°…“Õߧå- ‚√Õ–´‘μ‘°„πÕ–´‘‚μ‰π‰μ√≈å ( “√≈–≈“¬ °) ·≈–
ª√–°Õ∫∑“߇§¡’ ‚¥¬π” “√ °—¥®“° à«πμà“ß Ê √âÕ¬≈– 0.1 °√¥‰μ√ø≈ŸÕÕ‚√Õ–´‘μ‘°„ππÈ”( “√≈–≈“¬

¢Õß∑Õßæ—π™—Ëß¡“≈–≈“¬¥â«¬‡¡∑“πÕ≈∑’˧«“¡‡¢â¡¢âπ ¢) °√“‡¥’¬π∑å„πÕ—μ√“‡æ‘Ë¡§ß∑’Ë (linear gradient)
20 ¡‘≈≈‘°√—¡μàÕ¡‘≈≈‘≈‘μ√ °√Õߺà“π polyamide Õ—μ√“ à«π®“° (°:¢) 50:50 ‡ªìπ 80:20 „π‡«≈“ 10

membrane filter ¢π“¥ 0.45 ‰¡‚§√‡¡μ√ „™â π“∑’ ·≈–®“° 80:20 ‡ªìπ 100:0 „π‡«≈“®“° 10 π“∑’∂÷ß
μ—«Õ¬à“ß “√ °—¥ª√‘¡“μ√ 30 ‰¡‚§√≈‘μ√μàÕ°“√©’¥ 30 π“∑’ §«“¡‡√Á«¢Õß«—Ø¿“§‡§≈◊ËÕπ∑’Ë 1 ¡‘≈≈‘≈‘μ√
1 §√—Èß «‘‡§√“–Àå¥â«¬§Õ≈—¡πå™π‘¥√’‡«Õ√å ‡ø μàÕπ“∑’ ®“° “√ °—¥‡Õ∑‘≈Õ–´‘‡μ∑ª√–¡“≥ 74

(VertiSep TM UPS C18 HPLC COLUMN 4.6 × ¡‘≈≈‘°√—¡ ‰¥â “√ 1, 2, 3 ·≈– 4 ∑’Ë¡’≈—°…≥–°÷Ëß
250 mm, 5 μm) ∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕß (25 Õß»“‡´≈‡´’¬ ) ‡À≈« ’‡À≈◊Õß â¡πÈ”Àπ—° 4.1, 5.1, 31.0 ·≈– 3.4
„™â«—Ø¿“§‡§≈◊ËÕπ∑’˪√–°Õ∫¥â«¬√âÕ¬≈– 0.1 °√¥ ¡‘≈≈‘°√—¡ μ“¡≈”¥—∫ °“√«‘‡§√“–Àå Ÿμ√‚§√ß √â“ß
1
‰μ√ø≈ŸÕÕ‚√Õ–´‘μ‘°„πÕ–´‘‚μ‰π‰μ√≈å ( “√≈–≈“¬ °) ∑“߇§¡’‚¥¬°“√μ√«®«‘‡§√“–Àå¥â«¬‡∑§π‘§ MS, H
13
·≈–√âÕ¬≈– 0.1 °√¥‰μ√ø≈ŸÕÕ‚√Õ–´‘μ‘°„ππÈ” NMR, ·≈– C NMR spectroscopies ·≈â«π”
( “√≈–≈“¬ ¢) °√“‡¥’¬π∑å„πÕ—μ√“‡æ‘Ë¡§ß∑’Ë (linear ¢âÕ¡Ÿ≈¢ÕßπÈ”Àπ—°‚¡‡≈°ÿ≈·≈– NMR ‡ª§μ√—¡®“°

gradient) Õ—μ√“ à«π®“° (°:¢) 60:40 ‡ªìπ 75:25 DEPT, HMQC, HMBC ·≈– COSY ¡“„™â‡æ◊ËÕ
„π‡«≈“ 25 π“∑’ ·≈–®“° 75:25 ‡ªìπ 100:0 „π ª√–°Õ∫°“√«‘‡§√“–Àå Ÿμ√‚§√ß √â“ß∑“߇§¡’

‡«≈“®“° 25 π“∑’∂÷ß 35 π“∑’ ®“°π—Èπ„™â 100:0 μàÕ Rhinacanthin Q (1): Ÿμ√‚¡‡≈°ÿ≈ C H O 7
28 26
+ 1
®π∂÷ß 45 π“∑’ §«“¡‡√Á«¢Õß«—Ø¿“§‡§≈◊ËÕπ∑’Ë 1 ESI-MS: m/z 497 [M+Na] . H NMR (400 MHz,
¡‘≈≈‘≈‘μ√μàÕπ“∑’ «—¥°“√¥Ÿ¥°≈◊π· ßÕ—≈μ√“‰«‚Õ‡≈μ CDCl ) δ 1.16 (6H, s, 2 × 10-CH ), 2.79 (2H, s,
3 3
∑’˧«“¡¬“«§≈◊Ëπ 220 π“‚π‡¡μ√ H-9), 3.95 (3H, s, 1'-OCH ), 3.97 (3H, s, 4'-
3
OCH ), 4.16 (2H, s, 11-OCH ), 7.14 (1H, s, H-
3 2
°“√·¬°∫√‘ ÿ∑∏‘Ï “√®“°√“°∑Õßæ—π™—Ëß
3'), 7.39 (1H, t, J = 7.4 Hz, H-7), 7.45 (1H, s, 2-
π”√“°∑Õßæ—π™—Ëß·Àâß (150 °√—¡) ¡“ °—¥‚¥¬ OH), 7.48 (1H, t, J = 7.4 Hz, H-6), 7.55 (2H, m
°“√À¡—°¥â«¬‡Õ∑“πÕ≈ 3 ≈‘μ√‡ªìπ‡«≈“ 1 «—π ∑” overlap, H-6' & H-7'), 7.85 (1H, d, J = 7.6 Hz,

°“√ °—¥´È” 3 §√—Èß √«¡·≈–°√Õß “√ °—¥‡Õ∑“πÕ≈ H-8), 7.90 (1H, d, J = 7.6 Hz, H-5), 8.08 (1H,
∑’ˉ¥â 𔉪√–‡À¬¿“¬„μâ ÿ≠≠“°“»∑’ËÕÿ≥À¿Ÿ¡‘ 37 dd, J = 7.2, 2.1 Hz, H-8'), 8.17 (1H, dd, J = 7.3,
13
Õß»“‡´≈‡´’¬ ‰¥â “√ °—¥À¬“∫ 23.1 °√—¡ ®“°π—Èπ 2.1 Hz, H-5') C NMR (400 MHz, CDCl )
3
°—¥·¬°¥â«¬πÈ”·≈–‡Õ∑‘≈Õ–´‘‡μ∑ ‰¥â “√ °—¥‡Õ∑‘≈ δ 25.6 (2C, 2 × 12-CH ), 32.6 (C-9), 37.2 (C-
3
Õ–´‘‡μ∑πÈ”Àπ—° 6.0 °√—¡ π” à«π∑’Ë≈–≈“¬„π‡Õ∑‘≈ 10), 55.7 (4'-OCH ), 63.4 (1'-OCH ), 73.8 (C-
3 3