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√Ÿª∑’Ë 3 HPLC fingerprint ¢Õß√“°∑Õßæ—π™—Ëß
∑’Ë °—¥¥â«¬‡Õ∑“πÕ≈·≈– °—¥·¬°
μàե⫬‡Õ∑‘≈Õ–´‘‡μ∑

μ“√“ß∑’Ë 2 º≈°“√«‘‡§√“–Àåª√‘¡“≥ rhinacanthins „π “√ °—¥∑Õßæ—π™—Ëߥ⫬«‘∏’ HPLC

§à“‡©≈’ˬ peak area ± SD (mAU x min)* ¢Õß rhinacanthins
μ—«Õ¬à“ß “√ °—¥¢Õß∑Õßæ—π™—Ëß
1 2 3 4 √«¡

“√ °—¥‡Õ∑“πÕ≈·≈–‡Õ∑‘≈Õ–´‘‡μ∑¢Õß√“° 346.14 ± 19.43 270.41 ± 19.37 1204.11 ± 8.18 249.40 ± 13.26 2070.05 ± 43.88
“√ °—¥‡Õ∑“πÕ≈¢Õß√“° 231.79 ± 4.10 175.27 ± 1.87 1024.32 ± 14.72 176.65 ± 3.01 1608.03 ± 12.50
“√ °—¥‡Õ∑“πÕ≈·≈–‡Õ∑‘≈Õ–´‘‡μ∑¢Õß„∫ - 23.41 ± 2.03 324.08 ± 14.81 34.03 ± 2.51 318.52 ± 18.99
“√ °—¥‡Õ∑“πÕ≈¢Õß„∫ - 16.31 ± 2.95 215.23 ± 6.56 23.50 ± 2.05 255.04 ± 0.59
“√ °—¥‡Õ∑“πÕ≈¢Õß≈”μâπ - - - - -
* §à“‡©≈’ˬ®“°°“√«‘‡§√“–Àå 3 §√—Èß

„∫·≈–√“°∑Õßæ—π™—Ëß¡’ “√°≈ÿà¡ rhinacanthins «‘‡§√“–Àå Ÿμ√‚§√ß √â“ß∑“߇§¡’ “¡“√∂·¬° “√∑’Ë¡’
‡ªìπ à«πª√–°Õ∫ ‚¥¬¡’ —¥ à«πª√‘¡“≥ “√∑’Ë·μ° ≈—°…≥–°÷Ë߇À≈« ’‡À≈◊Õß â¡‰¥â®”π«π 4 ™π‘¥ ‰¥â·°à
μà“ß°—π ¥—ß· ¥ß„πμ“√“ß∑’Ë 2 ·≈–æ∫«à“ “√ °—¥ rhinacanthin Q (1), rhinacanthin B (2),

®“°√“°∑Õßæ—π™—Ëß¡’ª√‘¡“≥¢Õß “√°≈ÿà¡π’È¡“°°«à“ rhinacanthin C (3), ·≈– rhinacanthin N (4)
“√ °—¥®“° à«π„∫ „π¢≥–∑’Ë “√ °—¥®“° à«π (√Ÿª∑’Ë 4) ‚¥¬ª√‘¡“≥¢Õß “√∑’Ë·¬°‰¥â‡¡◊ËÕ§”π«≥
≈”μâπ‰¡àæ∫ “√°≈ÿà¡¥—ß°≈à“« ‡∑’¬∫°—∫πÈ”Àπ—°‡√‘Ë¡μâπ¢Õß√“°·Àâߧ‘¥‡ªìπ 0.22%,

‡¡◊ËÕπ” “√ °—¥®“° à«π√“°¢Õß∑Õßæ—π™—Ëß¡“ 0.28%, 1.68% ·≈– 0.18% w/w μ“¡≈”¥—∫ (μ“√“ß
·¬° “√∫√‘ ÿ∑∏‘ϥ⫬«‘∏’∑“ß‚§√¡“‚μ°√“øï‡æ◊ËÕ ∑’Ë 1)