Page 43 - วารสารปีที่16ฉบับที่2

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‡ªìπ‡™◊ÈÕÀ≈—°∑’Ë°àÕ„À⇰‘¥‚√§‡°≈◊ÈÕπ¥â«¬ ¡“°°«à“‡ªìπæ‘…μàÕ‡´≈≈媰μ‘∑’ËÕ¬Ÿà¿“¬„π™àÕߪ“° ‚¥¬
‡¡◊ËÕπ” “√ °—¥‡Õ∑“πÕ≈¢Õß à«π„∫·≈–√“° rhinacanthin C · ¥ßƒ∑∏‘ϧ«“¡‡ªìπæ‘…μàÕ‡´≈≈å
¡“ °—¥·¬°μàե⫬‡Õ∑‘≈Õ–´‘‡μ∑ æ∫«à“ “√ °—¥∑—Èß ¡–‡√Áß∑’Ë¡’§à“ tumor-specific cytotoxicity Ÿß
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„∫·≈–√“°„π à«π∑’Ë≈–≈“¬„π‡Õ∑‘≈Õ–´‘‡μ∑¡’§«“¡ ‡∑’¬∫‡∑à“°—∫¬“μâ“π¡–‡√ÁßÀ≈“¬™π‘¥ „π°“√
·√ß„π°“√μâ“π‡™◊ÈÕ√“ Ÿß¢÷Èπ · ¥ß„Àâ‡ÀÁπ«à“ “√μâ“π «‘‡§√“–Àå HPLC fingerprint ¢Õß “√ °—¥„∫∑Õß-
‡™◊ÈÕ√“„π∑Õßæ—π™—Ë߇ªìπ “√∑’Ë¡’¢—È«μË” ≈–≈“¬‰¥â¥’„π æ—π™—Ëß æ∫«à“¡’ 3 ‡ªìπ “√ÕÕ°ƒ∑∏‘ÏÀ≈—°‡™àπ°—π ‚¥¬¡’

μ—«∑”≈–≈“¬Õ‘π∑√’¬å ‡™àπ ‡Õ∑‘≈Õ–´‘‡μ∑ °“√ 2 ·≈– 4 ‡ªìπÕߧåª√–°Õ∫Õ¬Ÿà¥â«¬ ·μà¡’„πª√‘¡“≥
«‘‡§√“–Àå “√ °—¥∑Õßæ—π™—Ëߥ⫬‡∑§π‘§ HPLC ∑’ËπâÕ¬°«à“ “√ °—¥®“°√“°∑Õßæ—π™—Ëß ·≈–‰¡àæ∫ 1

‡æ◊ËÕ»÷°…“Õߧåª√–°Õ∫∑“߇§¡’æ∫«à“ “√ °—¥®“° „π “√ °—¥„∫∑Õßæ—π™—Ëß
„∫·≈–√“°∑Õßæ—π™—Ëß¡’≈“¬æ‘¡æå (fingerprint) ¢Õß ‡¡◊ËÕæ‘®“√≥“∂÷ß‚§√ß √â“ß∑“߇§¡’¢Õß 3 ´÷Ëß
‚§√¡“‚∑·°√¡∑’Ë¡’≈—°…≥–§≈⓬°—π·μà¡’ —¥ à«π · ¥ßƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“¥’∑’Ë ÿ¥‡¡◊ËÕ‡ª√’¬∫‡∑’¬∫°—∫ 1, 2

ª√‘¡“≥∑’Ë·μ°μà“ß°—π ®“°º≈°“√∑¥≈Õß “¡“√∂ ·≈– 4 æ∫«à“ 3 ¡’‚§√ß √â“ß∑’Ëμà“ß®“° 2 ‡©æ“–„π
√ÿª‰¥â«à“ “√ °—¥®“° à«π„∫·≈–√“°¡’Õߧå à«π¢Õß 2-hydroxy-1, 4-napthoquinone ‡∑à“π—Èπ
ª√–°Õ∫∑“߇§¡’‡ªìπ “√∑’Ë¡’ Ÿμ√‚§√ß √â“ß°≈ÿà¡ °“√∑’Ë 2 ¡’‚§√ß √â“߇ªìπ dimethyldihydropyrano-

‡¥’¬«°—π ‚¥¬æ∫«à“ “√ °—¥®“° à«π√“°∑Õßæ—π™—Ëß 1, 4-napthoquinone àߺ≈„Àâ¡’ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“≈¥
¡’ª√‘¡“≥¢Õß “√ª√–°Õ∫‡À≈à“π—Èπ¡“°°«à“ “√ °—¥ ≈ß¡“° ¢âÕ¡Ÿ≈π’È· ¥ß„Àâ‡ÀÁπ«à“ à«π 2-hydroxy-1, 4-
®“° à«π„∫‡¡◊ËÕ‡ª√’¬∫‡∑’¬∫®“°§à“æ◊Èπ∑’Ë„μâæ’§ (peak napthoquinone skeleton ¡’§«“¡ ”§—≠μàÕ°“√

area) ¢Õß‚§√¡“‚∑·°√¡∑’˪√“°Ø „π¢≥–∑’Ë “√ ÕÕ°ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“¢Õß “√°≈ÿà¡π’È
°—¥®“° à«π≈”μâπ‰¡àæ∫ “√°≈ÿà¡¥—ß°≈à“« ‡¡◊ËÕπ” πÕ°®“° “√ °—¥√“°∑Õßæ—π™—Ëß¡’ƒ∑∏‘Ïμâ“π‡™◊ÈÕ

“√ °—¥√“°∑Õßæ—π™—Ëß¡“·¬°∫√‘ ÿ∑∏‘χæ◊ËÕ»÷°…“ Ÿμ√ √“∑’Ë·√ß°«à“ “√ °—¥®“°„∫·≈â« ‡¡◊ËÕ‡ª√’¬∫‡∑’¬∫
‚§√ß √â“ß∑“߇§¡’ æ∫«à“ “√μâ“π‡™◊ÈÕ√“∑’Ë·¬°‰¥â‡ªìπ ƒ∑∏‘Ï¢Õß “√ °—¥√“°∑Õßæ—π™—Ëß∑’Ë °—¥¥â«¬‡Õ∑“πÕ≈
“√„π°≈ÿà¡ naphthoquinone esters ‚¥¬¡’ 3 ·≈–‡Õ∑‘≈Õ–´‘‡μ∑°—∫ 3 ´÷Ë߇ªìπ “√ÕÕ°ƒ∑∏‘ÏÀ≈—°

‡ªìπ “√ÕÕ°ƒ∑∏‘ÏÀ≈—°„π°“√μâ“π‡™◊ÈÕ√“ ´÷Ëß¡’ª√‘¡“≥ æ∫«à“„Àâº≈„π°“√μâ“π‡™◊ÈÕ√“μàÕ‡™◊ÈÕ∑’Ë„™â∑¥ Õ∫
§‘¥‡ªìπ 1.68% w/w ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—°‡√‘Ë¡μâπ ‡∑’¬∫‡∑à“°—π (μ“√“ß∑’Ë 1) ¥—ßπ—Èπ ®÷ß “¡“√∂„™â “√

¢Õß√“°·Àâß ·≈–¡’ª√‘¡“≥¡“°°«à“ “√μâ“π‡™◊ÈÕ√“ °—¥√“°∑Õßæ—π™—Ëß∑’Ë °—¥¥â«¬‡Õ∑“πÕ≈·≈–‡Õ∑‘≈Õ–-
™π‘¥Õ◊Ëπ∑’Ëæ∫ ‰¥â·°à 1, 2 ·≈– 4 “√‡À≈à“π’ȇ§¬¡’ ´‘‡μ∑ ‚¥¬‰¡à®”‡ªìπμâÕߺà“π°“√·¬°∫√‘ ÿ∑∏‘ϥ⫬«‘∏’
√“¬ß“π¡“°àÕπ·≈â«„π°“√»÷°…“ƒ∑∏‘Ï∑“ß™’«¿“æ¢Õß ∑“ß‚§√¡“‚∑°√“øï πÕ°®“°π’È¢âÕ¡Ÿ≈ HPLC finger-
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√“°∑Õßæ—π™—Ëß∑’ˇ°Á∫®“°‰μâÀ«—π “√°≈ÿà¡π’ÈÀ≈“¬ print ¢Õß “√ °—¥ à«ππ’È· ¥ß„Àâ‡ÀÁπ«à“Õߧå-
™π‘¥ ‡™àπ rhinacanthins C, G, N ·≈– Q · ¥ß ª√–°Õ∫∑“߇§¡’¢Õß “√ °—¥∑’ˉ¥â¡’ 3 ‡ªìπ à«π
ƒ∑∏‘ϧ«“¡‡ªìπæ‘…∑’Ë®”‡æ“–μàÕ‡´≈≈å¡–‡√Áß∫“ß™π‘¥ ‡™àπ ª√–°Õ∫À≈—° ·≈–¡’ “√Õ◊Ëπ Ê º ¡Õ¬Ÿà‡æ’¬ß‡≈Á°πâÕ¬

‡´≈≈å¡–‡√Á߇¬◊ËÕ∫ÿ™àÕߪ“° (HSC-2, HSC-3 ·≈– (√Ÿª∑’Ë 3) ´÷Ëß ª√‘¡“≥¢Õß “√ °—¥√“°∑Õßæ—π™—Ëß∑’Ë
HSC-4) ·≈–‡´≈≈å¡–‡√Á߇¡Á¥‡≈◊Õ¥¢“« (HL-60) °—¥¥â«¬‡Õ∑“πÕ≈·≈–‡Õ∑‘≈Õ–´‘‡μ∑∑’ˉ¥â§‘¥‡ªìπ