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‡™◊ÈÕ®ÿ≈™’æ (DMSO) „À≥⧫“¡‡¢â¡¢âπ 10 ¡‘≈≈‘°√—¡μàÕ¡‘≈≈‘≈‘μ√

‡™◊ÈÕ√“·∫∫¡’‡ âπ„¬°≈ÿࡇ¥Õ√å¡“‚μ‰øμå ”À√—∫ ·≈–𔉪≈–≈“¬μàÕ„πÕ“À“√ RPMI-MOPS „Àâ¡’
„™â∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“ ‰¥â®“° ∂“∫—π«‘®—¬ §«“¡‡¢â¡¢âπ‡ªìπ Õ߇∑à“¢Õߧ«“¡‡¢â¡¢âπ∑’ËμâÕß°“√

«‘∑¬“»“ μ√å “∏“√≥ ÿ¢ °√¡«‘∑¬“»“ μ√å°“√·æ∑¬å ∑¥ Õ∫‚¥¬‡μ√’¬¡‡ªìπ two-fold serial dilution
ª√–°Õ∫¥â«¬ Trichophyton rubrum DMST ª√‘¡“μ√ 50 ‰¡‚§√≈‘μ√ „π 96-well culture plate
30263, Trichophyton mentagrophytes DMST ™π‘¥ U-shape wells (§«“¡‡¢â¡¢âπ ”À√—∫°“√

19735, ·≈– Microsporum gypseum DMST ∑¥ Õ∫Õ¬Ÿà„π™à«ß 0.78-100 ‰¡‚§√°√—¡μàÕ¡‘≈≈‘≈‘μ√)
21146 ‚¥¬∑”°“√‡æ“–‡≈’Ȭß∫π sabouraud dex- ∑”°“√∑¥ Õ∫μ—«Õ¬à“ß “√ °—¥∑Õßæ—π™—Ëß·μà≈–™π‘¥

trose agar slants ‡°Á∫√—°…“∑’ËÕÿ≥À¿Ÿ¡‘ 2-8 Õß»“ 3 ´È” „™â ketoconazole (§«“¡‡¢â¡¢âπ ”À√—∫°“√
‡´≈‡´’¬ ·≈–‡ª≈’ˬπÕ“À“√„À¡àÕ¬à“ßπâÕ¬∑ÿ° 2 ∑¥ Õ∫Õ¬Ÿà„π™à«ß 0.078-10 ‰¡‚§√°√—¡μàÕ¡‘≈≈‘≈‘μ√)
—ª¥“Àå ‡μ√’¬¡‡™◊ÈÕ ”À√—∫°“√∑¥ Õ∫‚¥¬‡®◊Õ®“ß ‡ªìπ positive control ·≈–„™â DMSO ‡ªìπ nega-

‡™◊ÈÕ·μà≈–™π‘¥„π√âÕ¬≈– 0.85 sodium chloride tive control „ à‡™◊ÈÕ∑’ˇμ√’¬¡‰«â ”À√—∫°“√∑¥ Õ∫
(NaCl) „Àâ¡’§«“¡¢ÿàπ‡¡◊ËÕ«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 530 ª√‘¡“μ√ 50 ‰¡‚§√≈‘μ√≈ß„π·μà≈–À≈ÿ¡ ∫à¡∂“¥
π“‚π‡¡μ√‡∑à“°—∫ 1 McFarland standard ‡æ◊ËÕ„À⠇擖‡≈’Ȭß∑’ËÕÿ≥À¿Ÿ¡‘ 35 Õß»“‡´≈‡´’¬ ‡ªìπ‡«≈“ 96-
6
¡’§«“¡Àπ“·πàπ¢Õ߇™◊ÈÕ 1-5 × 10 ‡´≈≈åμàÕ¡‘≈≈‘≈‘μ√ 120 ™—Ë«‚¡ß À≈—ß®“°§√∫°”Àπ¥°“√∫ࡇ≈’ȬßÕà“π
®“°π—Èπ‡®◊Õ®“߇™◊ÈÕÕ’°§√—Èߥ⫬ՓÀ“√ RPMI-MOPS º≈°“√∑¥ Õ∫‚¥¬¥Ÿ‡ âπ„¬¢Õ߇™◊ÈÕ√“∑’˪√“°Ø„π
(RPMI 1640 medium, without sodium bicar- À≈ÿ¡‡ªìπμ—«∫àß™’È°“√‡®√‘≠¢Õ߇™◊ÈÕ√“ §à“§«“¡‡¢â¡¢âπ

bonate ∑’Ë¡’ à«πª√–°Õ∫¢Õß 2 mM L-glutamine μË” ÿ¥∑’Ë “¡“√∂¬—∫¬—Èß°“√‡®√‘≠¢Õ߇™◊ÈÕ (Minimum
·≈– 165 mM morpholinepropanesulfonic acid) Inhibitory Concentration, MIC) μ—¥ ‘π®“°

„πÕ—μ√“ à«π 1:50 §«“¡‡¢â¡¢âπμË” ÿ¥∑’ˉ¡à¡’°“√‡®√‘≠¢Õ߇™◊ÈÕ√“„πÀ≈ÿ¡
(‰¡àæ∫‡ âπ„¬¢Õ߇™◊ÈÕ√“„πÀ≈ÿ¡‡¡◊ËÕ —߇°μ¥â«¬μ“‡ª≈à“)
°“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“‚¥¬«‘∏’ Broth
microdilution method º≈°“√»÷°…“

°“√∑¥ Õ∫„™â«‘∏’°“√∑’Ë¥—¥·ª≈ß¡“®“°«‘∏’°“√ °“√ °—¥∑Õßæ—π™—Ëߥ⫬‡Õ∑“πÕ≈‰¥âª√‘¡“≥ “√

μ“¡ The Clinical and Laboratory Standard In- °—¥‡¡◊ËÕ§”π«≥‡∑’¬∫°—∫πÈ”Àπ—°·Àâß®“° à«πμà“ß Ê
stitute (CLSI) guideline (Document M38- ¢Õßæ◊™ (% yields, w/w) ¥—ßπ’È „∫ 8.07%, ≈”μâπ
[19]
A2) ‚¥¬‡μ√’¬¡ “√ °—¥∑Õßæ—π™—Ëß®”π«π 5 4.84% ·≈– √“° 8.43% ‡¡◊ËÕπ” “√ °—¥‡Õ∑“πÕ≈
μ—«Õ¬à“ß ‰¥â·°à “√ °—¥‡Õ∑“πÕ≈®“°„∫ ≈”μâπ √“° ®“° à«π„∫·≈–√“°¡“ °—¥·¬°μàÕ¥â«¬μ—«∑”≈–≈“¬
·≈– “√ °—¥‡Õ∑‘≈Õ–´‘‡μ∑®“°„∫·≈–√“° ·≈– “√ Õ‘π∑√’¬å§◊Õ‡Õ∑‘≈Õ–´‘‡μ∑ æ∫«à“ª√‘¡“≥ “√ °—¥„π
rhinacanthins 4 μ—«Õ¬à“ß ‰¥â·°à rhinacanthins à«π∑’Ë≈–≈“¬„π‡Õ∑‘≈Õ–´‘‡μ∑‡¡◊ËÕ§”π«≥‡∑’¬∫°—∫

Q B C ·≈– N ”À√—∫°“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“ πÈ”Àπ—°·ÀâߢÕß„∫§‘¥‡ªìπ 2.82% ·≈–√“° 2.92%
‚¥¬≈–≈“¬ “√ °—¥¥â«¬ dimethyl sulfoxide ¥—ß· ¥ß„πμ“√“ß∑’Ë 1 °“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“