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√Ÿª∑’Ë 2 TLC chromatogram ¢Õß “√ °—¥‡¡∑“πÕ≈®“°º≈¬Õ
1. “√ °—¥‡¡∑“πÕ≈®“°º≈¬Õ
2. “√ ‚°‚æ‡≈∑‘π
°“√μ√«®«—¥
A. UV 254 π“‚π‡¡μ√
B. UV 365 π“‚π‡¡μ√
C. Anisaldehyde-H SO , visible
2 4
D. Anisaldehyde-H SO , UV 365 π“‚π‡¡μ√
2 4

¡‘≈≈‘≈‘μ√ μm ·≈–«—Ø¿“§‡§≈◊ËÕπ∑’˪√–°Õ∫¥â«¬ A. ‡¡∑“πÕ≈-

°“√‡μ√’¬¡ “√≈–≈“¬¡“μ√∞“π Õ–´’‚μ‰π‰μ√≈å Õ—μ√“ à«π 1:1 ·≈– B. πÈ”°”®—¥Õ‘ÕÕπ
‡μ√’¬¡ “√≈–≈“¬ “√ ‚°‚æ‡≈∑‘π„π‡¡∑“πÕ≈ ∑” gradient elution Õ—μ√“°“√‰À≈ 0.45 ¡‘≈≈‘≈‘μ√/
®”π«π 6 √–¥—∫§«“¡‡¢â¡¢âπ „π™à«ß 8-48 ‰¡‚§√°√—¡/ π“∑’ ·≈–μ√«®«—¥ “√¥â«¬ UV ∑’˧«“¡¬“«§≈◊Ëπ 345

¡‘≈≈‘≈‘μ√ π“‚π‡¡μ√ ‚¥¬ peak ¢Õß “√ ‚°‚æ‡≈∑‘π®–ª√“°Ø∑’Ë
√–∫∫‚§√¡“‚∑°√“øï retention time ‡∑à“°—∫ 1.09 π“∑’ chromatogram
π” “√≈–≈“¬μ—«Õ¬à“ß∑’ˉ¥â ª√‘¡“μ√ 3 ¢Õß “√≈–≈“¬μ—«Õ¬à“ߺ≈¬Õ ¥—ß· ¥ß„π√Ÿª∑’Ë 3

‰¡‚§√≈‘μ√ ©’¥‡¢â“ Ÿà√–∫∫‚§√¡“‚∑°√“øï´÷Ëߪ√–°Õ∫ 2.4 °“√∑¥ Õ∫§«“¡„™â‰¥â¢Õß«‘∏’
¥â«¬ §Õ≈—¡πå™π‘¥ BEH C18, 2.1 × 100 mm, 1.7 æ∫«à“ calibration curve ¢Õß “√ ‚°‚æ-