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             many), chloroform AR (Merck, Germany), DI

             Water, anisaldehyde (Fluka, Switzerland), sul-
             furic acid (Labscan, Thailand)

                 4. ‡§√◊ËÕß¡◊Õ
                         TM
                 Acquity  Ultra Performance Liquid           √Ÿª∑’Ë 1 ‚§√ß √â“ß∑“߇§¡’¢Õß ‚°‚æ‡≈∑‘π
             Chromatograph-PDA detector (Waters, USA)

             ·≈– Empower 2 software, ‡§√◊ËÕß™—Ë߉øøÑ“ §«“¡   2. «‘∏’«‘‡§√“–Àåª√‘¡“≥ ‚°‚æ‡≈∑‘π„πº≈¬Õ
             ≈–‡Õ’¬¥ 0.01 ¡‘≈≈‘°√—¡ (Mettler-Toledo, Swit-      2.1 °“√æ—≤π“«‘∏’«‘‡§√“–Àåª√‘¡“≥ “√
                                                                         [10]
             zerland)                                     ‚°‚æ‡≈∑‘π„πº≈¬Õ
                 5. «— ¥ÿ«‘∑¬“»“ μ√å·≈–‡§√◊ËÕß·°â«              °. °“√‡≈◊Õ°™π‘¥¢Õßμ—«∑”≈–≈“¬„π°“√
                         TM
                 Acquity  UPLC BEH C18, 2.1 × 100         °—¥
             mm, 1.7 μm (Waters, USA), PTFE syringe fil-        ∑”°“√»÷°…“º≈¢Õß°“√ °—¥μ—«Õ¬à“ߺ≈¬Õ
             ter  ”À√—∫°√Õß “√≈–≈“¬μ—«Õ¬à“ß, TLC plate ™π‘¥  500 ¡‘≈≈‘°√—¡ ¥â«¬μ—«∑”≈–≈“¬Õ‘π∑√’¬å™π‘¥μà“ß Ê
             Silica gel GF254 precoated (E. Merck, Ger-  ‰¥â·°à ‡¡∑“πÕ≈ ‡Õ∑‘≈Õ–´’‡μ∑ ·≈–πÈ” ª√‘¡“μ√ 30

             many), °√–¥“…°√Õß ‡∫Õ√å 4 (Whatman, UK),    ¡‘≈≈‘≈‘μ√ ∑’ˇ«≈“ 60 π“∑’ °√Õß ®“°π—Èπ  “√ °—¥
             PVDF membrane filter 0.2 μm  ”À√—∫°√Õßμ—«   ‡¡∑“πÕ≈π”¡“ª√—∫ª√‘¡“μ√¥â«¬‡¡∑“πÕ≈®π§√∫ 50
             ∑”≈–≈“¬, Nylon membrane filter 0.45 μm (Pall  ¡‘≈≈‘≈‘μ√  “√ °—¥‡Õ∑‘≈Õ–´’‡μ∑π”¡“√–‡À¬·Àâß

             Corporation, USA)  ”À√—∫°√Õß DI water, Tu-  ·≈â«≈–≈“¬°≈—∫·≈–ª√—∫ª√‘¡“μ√¥â«¬‡¡∑“πÕ≈®π
             berculin syringe  ”À√—∫¥Ÿ¥ “√≈–≈“¬μ—«Õ¬à“ß  §√∫ 50 ¡‘≈≈‘≈‘μ√  à«π “√ °—¥πÈ”π”¡“ª√‘¡“μ√

             „π¢—ÈπμÕπ°“√‡μ√’¬¡μ—«Õ¬à“ß, volumetric flask,  ¥â«¬πÈ”®π§√∫ 50 ¡‘≈≈‘≈‘μ√ ·≈⫇ª√’¬∫‡∑’¬∫º≈
             pipette ·≈–‡§√◊ËÕß·°â«                      ¢Õßμ—«∑”≈–≈“¬μàÕ§«“¡ “¡“√∂„π°“√ °—¥ “√
                                                          ‚°‚æ‡≈∑‘π®“°º≈¬Õ ‚¥¬°“√©’¥ “√≈–≈“¬
             «‘∏’°“√»÷°…“
                                                         μ—«Õ¬à“߇¢â“ Ÿà√–∫∫ UPLC ·≈⫇ª√’¬∫ peak area
                 1. «‘∏’«‘‡§√“–À凙‘ߧÿ≥¿“æ¢Õߺ≈¬Õ¥â«¬«‘∏’  ¢Õß “√ ‚°‚æ‡≈∑‘π®“° “√ °—¥μà“ß Ê ª√–°Õ∫°—∫

             √ߧ‡≈¢º‘«∫“ß (TLC)                          peak shape  ‘Ë߇®◊ÕªπÕ◊Ëπ Ê (impurity) ·≈–
                 «‘∏’«‘‡§√“–À凙‘ߧÿ≥¿“æ¥â«¬«‘∏’ TLC ‰¥â∂Ÿ°  §«“¡§ßμ—«¢Õß “√≈–≈“¬μ—«Õ¬à“ß
             æ—≤π“¢÷Èπ‡æ◊ËÕ„™â ”À√—∫°“√μ√«®‡Õ°≈—°…≥å∑“߇§¡’     ¢. °“√»÷°…“√–¬–‡«≈“∑’ˇÀ¡“– ¡„π°“√

             ¢Õߺ≈¬Õ ‚¥¬„™â “√ ‚°‚æ‡≈∑‘π ´÷Ë߇ªìπ “√ ”§—≠   °—¥
             ∑’ËÕÕ°ƒ∑∏‘Ï„πº≈¬Õ‡ªìπ chemical marker              ∑”°“√»÷°…“º≈¢Õß°“√ °—¥μ—«Õ¬à“ߺ≈¬Õ
             ‚§√ß √â“ß∑“߇§¡’¢Õß “√ ‚°‚æ‡≈∑‘π ¥—ß· ¥ß„π  1 °√—¡ ¥â«¬‡¡∑“πÕ≈ª√‘¡“μ√ 30 ¡‘≈≈‘≈‘μ√ ‚¥¬«‘∏’

             √Ÿª∑’Ë 1                                    μâ¡ °—¥‡ªìπ‡«≈“ 30, 60 ·≈– 120 π“∑’ °√Õß
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