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Chemical Quality of Noni Fruits
Apirak Sakpetch*, Duangpen Pattamadilok, Peradham Thiemthieprat, Sakwichai Ontong,
Nuchattra Chansuvanich
Medicinal Plant Research Institute, Department of Medical Sciences, Nonthaburi 11000, Thailand
*Corresponding author: apirak.s@dmsc.mail.go.th

Abstract

The objectives of the study on chemical quality of noni fruits were to investigate qualitative analysis
method using TLC technique, to develop an analytical method for determining the amounts of active
compounds and to determine the amounts of active compounds in noni fruits using the developed method.
The determination was done on 0.5 g of powdered noni, extracted with methanol (20 ml), and then a TLC
analysis was performed on silica gel GF254, using toluene-diethyl ether (2:3) as mobile phase. An analytical
method for scopoletin content using UPLC was developed. Sample preparation was undertaken by refluxing
1 g of powdered noni fruit in 30 ml of methanol for 30 min., filtering, evaporating and adjusting to 25 ml.
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Then 2 μl of sample solution was subjected to UPLC analysis, using Acquity BEH C18 column, 2.1 × 100
mm, 1.7 μm as stationary phase. While (a) methanol-acetonitrile (1:1) and (b) distilled water were used as
mobile phase. Gradient elution was performed with a flow rate set at 0.45 ml/min. Detection was conducted
on UV345 nm. In the TLC analysis, spots of scopoletin appeared at R value of 0.3. The analytical method
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for scopoletin content using UPLC was developed and validated. Linearity was established for scopoletin at
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the concentration range of 8-48 μg/ml with a coefficient of determination (R ) value of 0.9995. Percent
recovery was in the range of 99-105%. The LOD and LOQ were 1 and 3 μg/ml, respectively. As a result of
scopoletin analysis in 17 samples of noni fruits, the amounts of this compound were in the range of 0.02-
0.07% w/w. The result of this study will be useful for chemical quality control of noni fruits.

Key words: noni fruits, Morinda citrifolia, Rubiaceae, scopoletin, quantitative analysis

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