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Journal of Thai Traditional & Alternative Medicine Vol. 8 Nos. 2-3 May-December 2010 ÒıÛ
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μâ“π‡™◊ÈÕ®ÿ≈‘π∑√’¬å ¬—∫¬—Èß°“√¥Ÿ¥´÷¡§Õ‡≈ ‡μÕ√Õ≈ ¬—∫¬—Èß ·≈– Silica gel GF254 precoated plate, 0.25 mm
‡Õπ‰´¡å pancreatic lipase ·≈–¬—∫¬—È߇Õπ‰´¡å HMG CoA (E. Merck, Germany)
Ù
reductase ‡ªìπμâπ
«‘∏’°“√»÷°…“
°“√«‘®—¬π’È®÷ß¡’«—μ∂ÿª√– ߧå‡æ◊ËÕ»÷°…“ƒ∑∏‘Ï≈¥‰¢¡—π„π
À≈Õ¥∑¥≈ÕߢÕß “√ °—¥®“°„∫™–¡«ß ‚¥¬»÷°…“º≈μàÕ°“√ Ò. °“√ °—¥ “√
¥Ÿ¥´÷¡§Õ‡≈ ‡μÕ√Õ≈‡¢â“ Ÿà‡´≈≈å≈”‰ â‡æ“–‡≈’È¬ß Caco-2 ´÷Ë߇ªìπ °—¥„∫™–¡«ß¥â«¬ ˘ı% ‡Õ∑“πÕ≈ ¥â«¬«‘∏’ soxhlet
cell-based assay ·≈–»÷°…“º≈°“√¬—∫¬—Èß°“√∑”ß“π¢Õß extraction ·≈â«π” “√ °—¥∑’ˉ¥â‰ª√–‡À¬·Àâß¿“¬„μâ ÿ≠≠“°“»

‡Õπ‰´¡å pancreatic lipase ·≈– HMG Co-A reductase ‰¥â “√ °—¥‡Õ∑“πÕ≈ (GC-1) ´÷Ëßπ”¡“ partition μàե⫬
´÷Ë߇ªìπ in vitro enzymatic assay ·≈–‰¥â∑”°“√∑¥ Õ∫ ‡Œ°‡´π ‰¥§≈Õ‚√¡’‡∑π·≈–∫‘«∑“πÕ≈ ·≈â«π” “√ °—¥·μà≈–

‡∫◊ÈÕßμâπ‡æ◊ËÕÀ“°≈ÿà¡ “√ ”§—≠·≈–»÷°…“∑“ß惰…‡§¡’‡æ◊ËÕÀ“ à«π∑’ˉ¥â‰ª√–‡À¬·Àâ߉¥â “√ °—¥‡Œ°‡´π (GC-2), “√ °—¥
“√ÕÕ°ƒ∑∏‘Ï≈¥‰¢¡—πÀ√◊Õ “√∫àß™’È (marker) „π°“√§«∫§ÿ¡ ‰¥§≈Õ‚√¡’‡∑π (GC-3), “√ °—¥∫‘«∑“πÕ≈ (GC-4) ·≈– “√
§ÿ≥¿“æ„∫™–¡«ßμàÕ‰ª„πÕπ“§μ ·≈–¬—߇ªìπ°“√‡æ‘Ë¡‡μ‘¡ °—¥πÈ” (GC-5) πÈ”Àπ—° ˆ˘.Ú, ¯ı.ˆ˜, Ò˘ˆ.˜ı ·≈–

¢âÕ¡Ÿ≈∑“ß惰…‡§¡’¢Õß„∫™–¡«ß´÷Ë߬—ß¡’√“¬ß“π‰¡à¡“°π—° ÙÛ.˘ °√—¡ μ“¡≈”¥—∫ (§‘¥‡ªìπ√âÕ¬≈– Û.ˆÙ, Ù.ıÒ, Ò.Ûˆ
·≈– Ú.Ú˜ μ“¡≈”¥—∫ ‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—°„∫™–¡«ß·Àâß)
√–‡∫’¬∫«‘∏’»÷°…“ ı
Ú. °“√∑¥ Õ∫°≈ÿà¡ “√ ”§—≠„π„∫™–¡«ß
¡ÿπ‰æ√ »÷°…“°≈ÿà¡¢Õß “√– ”§—≠∑’Ëæ∫„π “√ °—¥μà“ß Ê ®“°„∫
„∫™–¡«ß ¥ πÈ”Àπ—° Ò °‘‚≈°√—¡ π”¡“≈â“ßπÈ”„Àâ –Õ“¥ ™–¡«ß ‰¥â·°à terpenoids-steroids, flavonoids, phenolics,

Õ∫∑’ËÕÿ≥À¿Ÿ¡‘ ı Õß»“‡´≈‡´’¬ ®π·Àâß π”‰ª∫¥À¬“∫‰¥â alkaloids, anthraquinones, saponins ·≈– amines-amino
ºß„∫™–¡«ß·Àâß πÈ”Àπ—° Ò.˘ °‘‚≈°√—¡ (§‘¥‡ªìπ√âÕ¬≈– Ò˘ acids ‚¥¬„™â phytochemical screening test
‡¡◊ËÕ‡∑’¬∫°—∫πÈ”Àπ—°„∫™–¡«ß ¥) Û. °“√‡μ√’¬¡ “√ °—¥‡æ◊ËÕ∑¥ Õ∫ƒ∑∏‘Ï≈¥‰¢¡—π„π

«— ¥ÿ·≈– “√‡§¡’ À≈Õ¥∑¥≈Õß
μ—«∑”≈–≈“¬Õ‘π∑√’¬å∑’Ë„™â„π°“√‡μ√’¬¡ “√ °—¥ ¡ÿπ‰æ√ “√ °—¥ GC-1, GC-2, GC-3 ·≈– GC-4 ‰¡à “¡“√∂
·≈–·¬° “√„Àâ∫√‘ ÿ∑∏‘Ï„™â commercial grade ‚¥¬°≈—Ëπ´È” ≈–≈“¬πÈ”‰¥â μâÕ߇μ√’¬¡„ÀâÕ¬Ÿà„π√Ÿª¢Õß “√ª√–°Õ∫‡™‘ß´âÕπ

°àÕππ”¡“„™â °—∫ polyvinylpyrrolidone 40,000 (PVP complex) ‚¥¬
DMSO-d ·≈– CD OD (Wilmad Labglass, USA) “√ °—¥·μà≈–™π‘¥®–¡’Õ—μ√“ à«π∑’ˇÀ¡“– ¡„π°“√‡°‘¥
6 3
”À√—∫°“√‡μ√’¬¡μ—«Õ¬à“߇æ◊ËÕ«‘‡§√“–Àå¥â«¬«‘∏’ NMR spec- “√ª√–°Õ∫‡™‘ß´âÕπ∑’Ë·μ°μà“ß°—π ”À√—∫ “√ °—¥ GC-5
troscopy “¡“√∂≈–≈“¬πÈ”‰¥â ®÷߉¡àμâÕ߇μ√’¬¡‡ªìπ PVP complex
“√‡§¡’Õ◊Ëπ Ê ‰¥â·°à anisaldehyde (Fluka, Germany) Ù. °“√»÷°…“§«“¡‡ªìπæ‘…μàÕ‡´≈≈å Caco-2

·≈– sulfuric acid (Labscan, Thailand), polyvinylpyr- „™â«‘∏’ MTT assay ‡æ◊ËÕ»÷°…“º≈¢Õß “√ °—¥®“°„∫
ˆ
rolidone 40,000 (Fluka, USA), BCA protein assay kit ™–¡«ßμàÕ cell viability ¢Õ߇´≈≈å Caco-2

(Pierce) (Thermo Fisher Scientific, USA), scintillation Ù.Ò °“√‡æ“–‡≈’Ȭ߇´≈≈å Caco-2
cocktail (Perkin Elmer, USA), HMG CoA reductase ‡≈’Ȭ߇´≈≈å Caco-2 ¥â«¬Õ“À“√‡≈’Ȭ߇´≈≈å DMEM/
assay kit (Sigma, USA), pancreatic lipase (Sigma, USA), F12 ∑’Ë¡’ à«πº ¡¢Õß Ò% FBS ·≈– Ò% penicillin-strep-

4- methylumbelliferyl oleate (Sigma, USA), pravastatin tomycin ∫à¡„π incubator ∑’ËÕÿ≥À¿Ÿ¡‘ Û˜˚´ ·≈– CO ı%
2
(Sigma, USA), orilstat (Roche, Korea) ‡¡◊ËÕ‡´≈≈å Caco-2 ‡®√‘≠∑—Ë«®“π‡≈’È¬ß ∑”°“√ subculture ∑ÿ°
2+
«— ¥ÿ«‘∑¬“»“ μ√å∑’Ë„™â ‰¥â·°à silica gel 60 (E. Merck, Û-Ù «—π ‚¥¬ detach ‡´≈≈å¥â«¬ .Úı% trypsin „π Ca -,
2+
Garmany), Sephadex LH20 (GE Healthcare, Sweden) Mg -free phosphate buffer ∑’Ë¡’ EDTA .Ú °./≈. ‡ªìπ