Page 47 - journal-8-23-Full

a ve
Me c ei
l r
&
T r d if
al
a t n
Journal of Thai Traditional & Alternative Medicine Vol. 8 Nos. 2-3 May-December 2010 Òı˜
control ‚¥¬ orilstat ∑’˧«“¡‡¢â¡¢âπ .Úı mM “¡“√∂ μ“√“ß∑’Ë ˆ º≈¢Õß “√ °—¥®“°„∫™–¡«ß ∑’˧«“¡‡¢â¡¢âπ Ò ¡§°./
¡≈. μàÕ°“√∑”ß“π¢Õ߇Õπ‰´¡å HMG CoA reductase
¬—∫¬—Èß°“√∑”ß“π¢Õß pancreatic lipase ‰¥â√âÕ¬≈– ˘Û.ÚÛ
º≈°“√¬—∫¬—Èß°“√∑”ß“π¢Õ߇Õπ‰´¡å pancreatic lipase ¢Õß % °“√¬—∫¬—È߇Õπ‰´¡å
≈”¥—∫∑’Ë “√ °—¥
“√ °—¥®“°„∫™–¡«ß· ¥ß„π μ“√“ß∑’Ë ı æ∫«à“ “√ °—¥ GC- HMG-CoA reductase
2 “¡“√∂¬—∫¬—Èß°“√∑”ß“π¢Õ߇Õπ‰´¡å‰¥â¥’∑’Ë ÿ¥¥â«¬§à“ IC50 Ò GC-1 ˘˜.ˆ ± ˘.ÛÒ
‡∑à“°—∫ ˆ˜.Ùı ¡§°./¡≈. Ú GC-2 ÒÒÙ.ÛÙ ± ÚÙ.ÙÛ
ˆ. º≈μàÕ°“√¬—∫¬—Èß°“√∑”ß“π¢Õ߇Õπ‰´¡å HMG CoA Û GC-3 ¯.ıı ± Òˆ.ˆı

reductase Ù GC-4 ¯.Ú˜ ± Ù.ˆÛ
®“°°“√»÷°…“º≈°“√¬—∫¬—Èß°“√∑”ß“π¢Õ߇Õπ‰´¡å HMG ı GC-5 ıˆ.Ò¯ ± ÒÒ.ˆÙ

μ“√“ß∑’Ë ˜ 500 MHz 1H NMR ·≈– 125 MHz 13C NMR ‡ª°μ√—¡¢Õß vitexin ·≈– orientin („π CD3OD+DMSO-d6)

Ò,ÒÒ ÒÒ
Vitexin Orientin
μ”·Àπàß
δ ppm δ ppm δ ppm δ ppm
H C H C
2 - 163.96 - 164.08

3 6.63 (1H, s) 102.44 6.63 (1H, s) 102.44
4 - 182.10 - 182.02
5 - 161.13 - 161.13
5-OH 13.15 (1H, s) - 13.15 (1H, s) -

6 6.26 (1H, s) 98.13 6.25 (1H, s) 98.13
7 - 162.55 - 163.96
7-OH 10.84 (1H, s) - - -
8 - 104.60 - 104.60
9 - 155.99 - 155.99
10 - 104.03 - 104.03
1′ - 121.61 - 121.61
2′ 8.01 (1H, d, J = 8.5 Hz) 128.97 7.47 (1H, s) 114.04
3′ 6.88 (1H, d, J = 8.5 Hz) 115.81 - 145.80
4′ - 160.38 - 149.60

4′-OH 10.35 (1H, s) - - -
5′ 6.88 (1H, d, J = 8.5 Hz) 115.81 6.85 (1H, d, J = 8.5 Hz) 115.64
6′ 8.01 (1H, d, J = 8.5 Hz) 128.97 7.52 (1H, d, J = 8.5 Hz) 119.36

Glc-1 4.67 (1H, d, J = 9.5 Hz) 73.38 4.67 (1H, d, J = 9.5 Hz) 73.38
Glc-2 3.82 (1H, t, J = 9.3 Hz ) 70.83 3.82 (1H, t, J = 9.3 Hz ) 70.76
Glc-3 78.66 78.75
Glc-4 3.0-3.6 70.54 3.0-3.6 70.69

Glc-5 81.84 81.98
Glc-6 61.29 61.63