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°“√ ∑ å π‰∑¬
√ “√°“√ ∑¬å·ºπ‰∑¬·≈–°“√ ∑¬å∑“å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
«“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬
¡ÿπ‰æ√ ‡°Á∫‰«â∑’ËÕÿ≥À¿Ÿ¡‘ -Ú˚´ ®π°«à“®–π”¡“„™â„π°“√∑¥ Õ∫ ‡¡◊ËÕ
∑”°“√«—¥°“√¥Ÿ¥´÷¡§Õ‡≈ ‡μÕ√Õ≈‚¥¬ª√–‡¡‘π√–¥—∫ ®–π”¡“„™â„Àâ≈–≈“¬„π DMEM/F12 ·≈– sonicate ·≈â«
¢ÕߧՇ≈ ‡μÕ√Õ≈∑’Ëμ‘¥©≈“°¥â«¬ “√°—¡¡—πμ√—ß ’∑’˺à“π‡¢â“ Ÿà 𔉪°√Õߺà“π .Ú μm filter membrane
‡´≈≈å Caco-2 „™â ezetimibe ‡ªìπ positive control Û.Ú °“√π”‡¢â“§Õ‡≈ ‡μÕ√Õ≈ (cholesterol uptake)
Û.Ò °“√‡μ√’¬¡ cholesterol micelles „π‡´≈≈å Caco-2
‚¥¬‡μ√’¬¡ Ú mM taurocholate, ı μM phosphati- ‡≈’Ȭ߇´≈≈å Caco-2 „π ÚÙ well plate ‚¥¬‡ª≈’ˬπ
3
dylcholine, Ò μM cholesterol ·≈– Ò μCi/ml [1,2- H] Õ“À“√‡≈’Ȭ߇´≈≈å∑ÿ° Ú-Û «—π ‡¡◊ËÕ‡´≈≈å¡’Õ“¬ÿ Ú-Û —ª¥“Àå ‡´≈≈å
cholesterol ¡“≈–≈“¬„π chloroform ·≈â«π”¡“º ¡°—π Caco-2 ®–‡°‘¥°“√ differentiation ‡ªìπ monolayer ´÷Ëß
„πÀ≈Õ¥·°â« ®“°π—Èπ 𔉪√–‡À¬¿“¬„μâ·°ä ‰π‚μ√‡®π ·≈– ‡≈’¬π·∫∫‡´≈≈å∑’˺π—ß≈”‰ â‡≈Á° ‡¡◊ËÕ®–«—¥°“√¥Ÿ¥´÷¡§Õ‡≈ -
μ“√“ß∑’Ë Ò °“√‡μ√’¬¡ “√ °—¥ ¡ÿπ‰æ√
Ò
≈”¥—∫∑’Ë √À— “√ ™◊ËÕ«‘∑¬“»“ μ√å/«ß»å ™◊ËÕ‰∑¬ à«π∑’Ë„™â ™π‘¥ “√ °—¥ «‘∏’°“√ °—¥ “√ °—¥ : PVP
Ò NN Nelumbo nucifera Gaertn. ∫—«À≈«ß „∫ ‡Õ∑“πÕ≈ Soxhlet Ò:Ù
ǧȌ Nelumbonaceae extraction
Ú GC Garcinia cowa Roxb. ex DC. ™–¡«ß „∫ ‡Õ∑“πÕ≈ Soxhlet Ò:Ù
ǧȌ Clusiaceae extraction
Û ABL-1 Averrhoa bilimbi L. μ–≈‘ߪ≈‘ß „∫ ‡Õ∑“πÕ≈ Soxhlet Ò:Ù
ǧȌ Oxalidaceae extraction
Ù ABL-2 Averrhoa bilimbi L. μ–≈‘ߪ≈‘ß „∫ πÈ” reflux -
ǧȌ Oxalidaceae
ı ABF Averrhoa bilimbi L. μ–≈‘ߪ≈‘ß º≈ πÈ” §—ÈππÈ” -
ǧȌ Oxalidaceae
ˆ MO-1 Moringa oleifera Lamk. ¡–√ÿ¡ „∫ ‡Õ∑“πÕ≈ Soxhlet Ò:Ù
ǧȌ Moringaceae extraction
˜ MO-2 Moringa oleifera Lamk. ¡–√ÿ¡ „∫ πÈ” reflux -
ǧȌ Moringaceae
¯ MC Morinda citrifolia L. ¬Õ „∫ ‡Õ∑“πÕ≈ Soxhlet Ò:Ù
ǧȌ Rubiaceae extraction
˘ PG Psidium guajava L. Ω√—Ëß „∫ ‡Õ∑“πÕ≈ Soxhlet Ò:Û
ǧȌ Myrtaceae extraction
Ò AG Alpinia galanga Sw. ¢à“ ‡Àßâ“ - SFE Ò:˘
ǧȌ Zingiberaceae
ÒÒ GP Gynostemma pentaphyllum Maniko. ªí≠®¢—π∏å à«π„μ⥑π ‡Õ∑“πÕ≈ Soxhlet Ò:Ù
ǧȌ Cucurbitaceae extraction
ÒÚ CH Citrus hystrix DC. ¡–°√Ÿ¥ „∫ ‡Õ∑“πÕ≈ À¡—° Ò:Ù
ǧȌ Rutaceae
ÒÛ TT-1 Tiliacora triandra Diels. ¬à“π“ß „∫ ‡Õ∑“πÕ≈ Soxhlet Ò:Ù
ǧȌ Menispermaceae extraction
ÒÙ TT-2 Tiliacora triandra Diels. ¬à“π“ß „∫ πÈ” reflux -
ǧȌ Menispermaceae