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ÒÙ¯   «“√ “√°“√·æ∑¬ ¬·º√ “√  · °“√≈–  ∑¬åå∑“߇≈◊Õ°                         ªï∑’Ë ¯©∫—∫∑’Ë Ú-Û æƒ…¿“§¡-∏—𫓧¡ ÚııÛ
                                             ∑“
                  °“√ ∑ å π‰∑¬
               √ “√°“√ ∑¬å·ºπ‰∑¬·≈–°“√ ∑¬å∑“å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
             «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬
              ¡ÿπ‰æ√                                            ‡°Á∫‰«â∑’ËÕÿ≥À¿Ÿ¡‘ -Ú˚´ ®π°«à“®–π”¡“„™â„π°“√∑¥ Õ∫ ‡¡◊ËÕ

                 ∑”°“√«—¥°“√¥Ÿ¥´÷¡§Õ‡≈ ‡μÕ√Õ≈‚¥¬ª√–‡¡‘π√–¥—∫    ®–π”¡“„™â„Àâ≈–≈“¬„π DMEM/F12 ·≈– sonicate ·≈â«
             ¢ÕߧՇ≈ ‡μÕ√Õ≈∑’Ëμ‘¥©≈“°¥â«¬ “√°—¡¡—πμ√—ß ’∑’˺à“π‡¢â“ Ÿà  𔉪°√Õߺà“π .Ú μm filter membrane
             ‡´≈≈å Caco-2 „™â ezetimibe ‡ªìπ positive control           Û.Ú °“√π”‡¢â“§Õ‡≈ ‡μÕ√Õ≈ (cholesterol uptake)

                    Û.Ò °“√‡μ√’¬¡ cholesterol micelles          „π‡´≈≈å Caco-2
                 ‚¥¬‡μ√’¬¡ Ú mM taurocholate, ı μM phosphati-          ‡≈’Ȭ߇´≈≈å Caco-2 „π ÚÙ well plate ‚¥¬‡ª≈’ˬπ
                                                         3
             dylcholine, Ò μM cholesterol ·≈– Ò μCi/ml [1,2- H]  Õ“À“√‡≈’Ȭ߇´≈≈å∑ÿ° Ú-Û «—π ‡¡◊ËÕ‡´≈≈å¡’Õ“¬ÿ Ú-Û  —ª¥“Àå ‡´≈≈å
             cholesterol ¡“≈–≈“¬„π chloroform ·≈â«π”¡“º ¡°—π    Caco-2 ®–‡°‘¥°“√ differentiation ‡ªìπ monolayer ´÷Ëß
             „πÀ≈Õ¥·°â« ®“°π—Èπ 𔉪√–‡À¬¿“¬„μâ·°ä ‰π‚μ√‡®π ·≈–  ‡≈’¬π·∫∫‡´≈≈å∑’˺π—ß≈”‰ â‡≈Á° ‡¡◊ËÕ®–«—¥°“√¥Ÿ¥´÷¡§Õ‡≈ -




             μ“√“ß∑’Ë Ò °“√‡μ√’¬¡ “√ °—¥ ¡ÿπ‰æ√

                                              Ò
              ≈”¥—∫∑’Ë √À—  “√  ™◊ËÕ«‘∑¬“»“ μ√å/«ß»å        ™◊ËÕ‰∑¬   à«π∑’Ë„™â  ™π‘¥ “√ °—¥  «‘∏’°“√ °—¥  “√ °—¥ : PVP
                Ò      NN     Nelumbo nucifera Gaertn.     ∫—«À≈«ß     „∫      ‡Õ∑“πÕ≈     Soxhlet      Ò:Ù
                              ǧȌ Nelumbonaceae                                           extraction
                Ú      GC     Garcinia cowa Roxb. ex DC.    ™–¡«ß      „∫      ‡Õ∑“πÕ≈     Soxhlet      Ò:Ù
                              ǧȌ Clusiaceae                                              extraction
                Û     ABL-1   Averrhoa bilimbi L.          μ–≈‘ߪ≈‘ß   „∫      ‡Õ∑“πÕ≈     Soxhlet      Ò:Ù
                              ǧȌ Oxalidaceae                                             extraction
                Ù     ABL-2   Averrhoa bilimbi L.          μ–≈‘ߪ≈‘ß   „∫         πÈ”       reflux       -
                              ǧȌ Oxalidaceae
                ı      ABF    Averrhoa bilimbi L.          μ–≈‘ߪ≈‘ß   º≈         πÈ”       §—ÈππÈ”      -
                              ǧȌ Oxalidaceae
                ˆ     MO-1    Moringa oleifera Lamk.        ¡–√ÿ¡      „∫      ‡Õ∑“πÕ≈     Soxhlet      Ò:Ù
                              ǧȌ Moringaceae                                             extraction
                ˜     MO-2    Moringa oleifera Lamk.        ¡–√ÿ¡      „∫         πÈ”       reflux       -
                              ǧȌ Moringaceae
                ¯      MC     Morinda citrifolia L.          ¬Õ        „∫      ‡Õ∑“πÕ≈     Soxhlet      Ò:Ù
                              ǧȌ Rubiaceae                                               extraction
                ˘      PG     Psidium guajava L.             Ω√—Ëß     „∫      ‡Õ∑“πÕ≈     Soxhlet      Ò:Û
                              ǧȌ Myrtaceae                                               extraction
               Ò      AG     Alpinia galanga Sw.            ¢à“      ‡Àßâ“       -          SFE        Ò:˘
                              ǧȌ Zingiberaceae
               ÒÒ      GP     Gynostemma pentaphyllum Maniko. ªí≠®¢—π∏å  à«π„μâ¥‘π  ‡Õ∑“πÕ≈  Soxhlet    Ò:Ù
                              ǧȌ Cucurbitaceae                                           extraction
               ÒÚ      CH     Citrus hystrix DC.            ¡–°√Ÿ¥     „∫      ‡Õ∑“πÕ≈       À¡—°       Ò:Ù
                              ǧȌ Rutaceae
               ÒÛ      TT-1   Tiliacora triandra Diels.     ¬à“π“ß     „∫      ‡Õ∑“πÕ≈     Soxhlet      Ò:Ù
                              ǧȌ Menispermaceae                                          extraction
               ÒÙ      TT-2   Tiliacora triandra Diels.     ¬à“π“ß     „∫         πÈ”       reflux       -
                              ǧȌ Menispermaceae
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