Page 41 - journal-8-23-Full
P. 41

&
                     al
                         T   r d if
                                a t n
                                            l r
                                              a ve
                                                       Me c ei
             Journal of Thai Traditional & Alternative Medicine                      Vol. 8 Nos. 2-3 May-December 2010 ÒıÒ
                                                                    Inhibitory effects of grape seed extract on lipases. Nutrition
             ∑“ß«‘∑¬“»“ μ√å‡æ◊ËÕ π—∫ πÿπ°“√„™â¬“®“° ¡ÿπ‰æ√‰∑¬μàÕ
                                                                    2003;19:876-9.
             ‰ª„πÕπ“§μ
                                                                 ı. Peffley DM, Gayen AK. Plant-derived monoterpenes suppress hamster
                                                                    kidney cell 3-hydroxy-3-methylglutaryl coenzyme a reductase systhesis
                           °‘μμ‘°√√¡ª√–°“»                          at the post-transcriptional level. J Nutr 2003;133:38-44.
                                                                 ˆ. Fuhrman B, Elis A, Aviram M. Hypocholesterolemic effect of lycopene
                 ·À≈àß∑ÿπ π—∫ πÿπ°“√«‘®—¬ß∫ª√–¡“≥·ºàπ¥‘π ª√–®”      and (-carotene is related to suppression of cholesterol synthesis and
                                                                    augmentation of LDL receptor activity in macrophages. Biomed Biophys
             ªïß∫ª√–¡“≥ æ.». 2552-2553.
                                                                    Res Commun 1997;233:658-62.
                                                                 ˜. Tuansulong KA, Hutadilok-Towatana N, Mahabusarakam W, Pinkaew
                                                                    D, Fujise K. Morelloflavone from Garcinia dulcis as a novel biflavonoid
             ‡Õ° “√Õâ“ßÕ‘ß
                                                                    inhibitor of HMG CoA reductase. Phytother Res 2011;25(3):424-8.
              Ò. Wang DQH. New concepts of mechanisms of intestinal cholesterol  ¯. Kirana C, Rogers PF, Bennett LE, Abeywardena MY, Patten GS.
                absorption. Ann Hepatol 2003;2:113-21.              Naturally derived micelles for rapid in vitro screening of potential
              Ú. Zheng C-D, DuanY-Q, Gao J-M, Ruan Z-G. Screening for anti-lipase  cholesterol-lowering bioactives. J. Agric Food Chem 2005;53:4623-7.
                properties of 37 traditional Chinese medicinal herbs. J Chin Med  ˘. Plumb JA, Milroy A, Kaye SB. Effect of the pH dependence of 3-(4,5-
                Assoc 2010;73:319-24.                               dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-formazan
              Û. Bhutani KK., Gohil VM. Natural products drug discovery research in  absorption on chemosensitivity determinated by a novel tetrazolium-
                India: status and appraisal. Indian J Experimental Biol 2010;48:199-  based assay. Cancer Res 1989;49:4435-40.
                207.                                            Ò. ‡μÁ¡  ¡‘μ‘π—π∑å. ™◊ËÕæ√√≥‰¡â·Ààߪ√–‡∑»‰∑¬. ©∫—∫·°â‰¢‡æ‘Ë¡‡μ‘¡.
              Ù. Moreno DA, Ilic N, Poulev A, Brasaemle DL, Fried SK, Raskin I.  °√ÿ߇∑æœ: ∫√‘…—∑ ª√–™“™π ®”°—¥; ÚıÙÙ.










                  Abstract
                       Screening of Cholesterol Uptake Inhibitor from Thai Medicinal Plant Extracts
                       Duangpen Pattamadilok* Somchit Niumsakul* Nanteetip Limpeanchob , Kornkanok Ingkaninan ,
                                                                                                  †
                                                                              †
                       Prapai Wongsinkongman*
                       *Medicinal Plant Research Institute, Department of Medical Sciences, Nonthaburi 11000
                       † Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000
                           The aim of this investigation was to screen Thai medicinal plant extracts for cholesterol uptake inhibi-
                       tory activity, a new therapeutic target in the treatment of hyperlipidemia.  The effects of 14 plant extracts on
                       cholesterol uptake inhibition were observed by determining the level of incorporated labelled cholesterol into
                       Caco-2 cells. Ezetimibe was used as positive control. At the concentration of 100 μg/ml, the ethanol extract of
                       Psidium guajava (PG) leaves showed the strongest activity at 43 percent cholesterol uptake inhibition. Water
                       extract and ethanol extract of Averrhoa bilimbi leaves (ABL-1 and ABL-2) and ethanol extract of Nelumbo
                       nucifera (NN) leaves displayed the inhibition between 34-36 percent.  Ethanol extract from leaves of Moringa
                       oleifera (MO-1), Morinda citrifolia (MC), Tiliacora triandra (TT-1) and water extract from fruits of A. bilimbi
                       (ABF) exhibited cholesterol uptake inhibition between 20 and 30 percent.  At the concentration of 500 μg/ml,
                       the cholesterol uptake inhibitory effect of MO-1 and TT-1 was increased to 51 and 37 percent, respectively.
                       The ethanol extract from leaves of Garcinia cowa (GC), Citrus hystrix (CH), the underground part of Gynostemma
                       pentaphyllum (GP), water extract from leaves of M. oleifera (MO-2), T. triandra (TT-2) and SFE extract of
                       Alpinia galanga (AG) showed no inhibitory activity. The result of this investigation has led to further isolation
                       of cholesterol uptake inhibitors from potential Thai medicinal plants.
                       Key words: Cholesterol uptake inhibition, Thai medicinal plants
   36   37   38   39   40   41   42   43   44   45   46