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Journal of Thai Traditional & Alternative Medicine Vol. 8 Nos. 2-3 May-December 2010 ÒıÒ
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Abstract
Screening of Cholesterol Uptake Inhibitor from Thai Medicinal Plant Extracts
Duangpen Pattamadilok* Somchit Niumsakul* Nanteetip Limpeanchob , Kornkanok Ingkaninan ,
†
†
Prapai Wongsinkongman*
*Medicinal Plant Research Institute, Department of Medical Sciences, Nonthaburi 11000
† Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000
The aim of this investigation was to screen Thai medicinal plant extracts for cholesterol uptake inhibi-
tory activity, a new therapeutic target in the treatment of hyperlipidemia. The effects of 14 plant extracts on
cholesterol uptake inhibition were observed by determining the level of incorporated labelled cholesterol into
Caco-2 cells. Ezetimibe was used as positive control. At the concentration of 100 μg/ml, the ethanol extract of
Psidium guajava (PG) leaves showed the strongest activity at 43 percent cholesterol uptake inhibition. Water
extract and ethanol extract of Averrhoa bilimbi leaves (ABL-1 and ABL-2) and ethanol extract of Nelumbo
nucifera (NN) leaves displayed the inhibition between 34-36 percent. Ethanol extract from leaves of Moringa
oleifera (MO-1), Morinda citrifolia (MC), Tiliacora triandra (TT-1) and water extract from fruits of A. bilimbi
(ABF) exhibited cholesterol uptake inhibition between 20 and 30 percent. At the concentration of 500 μg/ml,
the cholesterol uptake inhibitory effect of MO-1 and TT-1 was increased to 51 and 37 percent, respectively.
The ethanol extract from leaves of Garcinia cowa (GC), Citrus hystrix (CH), the underground part of Gynostemma
pentaphyllum (GP), water extract from leaves of M. oleifera (MO-2), T. triandra (TT-2) and SFE extract of
Alpinia galanga (AG) showed no inhibitory activity. The result of this investigation has led to further isolation
of cholesterol uptake inhibitors from potential Thai medicinal plants.
Key words: Cholesterol uptake inhibition, Thai medicinal plants