Page 43 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 18 ฉบับที่ 2

J Thai Trad Alt Med Vol. 18 No. 2 May-Aug 2020 265

of fibroblast cells by measuring the purple color in medicinal plants. In Table 1, antioxidant
of formazan crystals that are produced by mi- activity measured in DPPH and ABTS assays
tochondria enzyme. Low relative cell viability were described using the summary measures

implies that extracts have a high cytotoxic of IC50 and TEAC, respectively.
effect on fibroblast cells. T. triquetrum extracted by water showed

Figure 1 revealed that there were no cyto- the highest antioxidant activity with IC50
toxic effect on fibroblast cells for all concentra- value of 12.45 ± 0.14 mg/mL and TEAC value
tions of T. triquetrum extracts tested (0.2–100 of 0.85 ± 0.11 mM. While hexane extract of T.

mg/mL). Relative cell viability measurements triquetrum showed the lowest antioxidant
were 80% or greater for all concentrations and activity with IC50 value of 180.20 ± 1.79 mg/

among each of the extracts tested. Although mL and TEAC value of 5.50 ± 0.86 mM. [16]
T. triquetrum extracted by hexane showed the 3. Phosphodiesterase-5 inhibitory activity
lowest cell viability compared to other extracts, The phosphodiesterase-5 inhibitory

the relative cell viability of T. triquetrum ex- activity of T.triquetrum extracts is shown in
tracted by hexane was still more than 80%. [15] Table 2. We present the % inhibitory activity
2. Antioxidant activity at 50 mg/mL concentrations of the extract.

Testing antioxidant activity is the The PDE-5 inhibitory activity of T. triquetrum
primary screening tool used to identify in- extracts depended on the type of solvent
teresting herbs. DPPH and ABTS assays are that was used. Water extract of T. triquetrum

usually used for screening antioxidant activity presented the highest inhibition of PDE-5

Figure 1 Cytotoxic effect of T. triquetrum extracts on human fibroblast cells