Page 39 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 18 ฉบับที่ 2

J Thai Trad Alt Med Vol. 18 No. 2 May-Aug 2020 261

ability. It is often incorporated into a traditional tive products developed from T. triquetrum for
drug recipe for improving sexual and physical diabetic patient health promotion are promis-
performance. In addition, T. triquetrum is ing. Although recent in vitro studies revealed
[1,8]
traditionally used as an anti-microbial agent the beneficial effects of T. triquetrum, the
in aqueous herbal products. The herb prevents bioactivity of folk medicine formulations has

the growth of microorganisms and extends the been not identified.
products’expiration date, Moreover, topical This study will determine the bioactivity
applications of the herb have been used to of T. triquetrum extract in dosages and formu-

treat infectious wounds. [1,4,7] lations found in Thai folk medicine practice.
Researchers have also found this herb Specifically, we aim to investigate the herb’s

useful in food preservation and has effects antioxidant activity, anti-microbial activity,
on different types of cells. Researchers found and the inhibition activity of the PDE-5 en-
that maggot growth in pickled fish was limited zyme.

when T. triquetrum extract was incorporated
as an ingredient. Moreover, bacterial growth Methodology
was suppressed, so the food expiration date Materials

was extended. Recently, researchers revealed Tadehagi triquetrum (L.) H. Ohashi leaves
[9]
evidence that T. triquetrum contained ligand were collected and identified by a botanist of
and phenylated isoflavones that prevented Tungsleangluang National Park, Petchaboon

cell activity. They inhibited effects of chemi- Province, Thailand. DPPH (1,1-diphenyl-
cal toxicity on hepatocyte cells by decreasing 2-picrylhydrazyl), ABTS (2,2′-azino-bis-3-eth-

the expression of NFκB. Tadehaginosin and ylbenzthiazoline-6-sulphonic acid), MTT ([3-(4,
[10]
3,4-dihydro-4-(40-hydroxyphenyl)-5,7-dihy- 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-]
droxycoumarin were isolated from the aerial tetrazolium bromide) and 95% ethanol were

part of T. triquetrum. These two compounds AR grade and purchased from Sigma (Sigma-
displayed hypoglycemic activity by increas- Aldrich, Singapore)

ing glucose consumption and reducing blood
glucose levels. Phenylpropanoid glucosides Instruments
[11]
and tadehaginosides increased glucose uptake - UV-Visible spectrophotometer (Evolu-

by inducing peroxisome proliferator-activated tion 201, Thermo Scientific)
receptor γ (PPARγ) activity and glucose trans- - Microplate reader (Eon, Biotek)
porter-4 (GLUT-4) expression. The alterna-
[12]