Page 39 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 18 ฉบับที่ 2
P. 39

J Thai Trad Alt Med                                   Vol. 18  No. 2  May-Aug 2020  261




              ability. It is often incorporated into a traditional   tive products developed from T. triquetrum for
              drug recipe for improving sexual and physical   diabetic patient health promotion are promis-
              performance.  In addition, T. triquetrum is   ing. Although recent in vitro studies revealed
                          [1,8]
              traditionally used as an anti-microbial agent   the beneficial effects of T. triquetrum, the
              in aqueous herbal products. The herb prevents   bioactivity of folk medicine formulations has

              the growth of microorganisms and extends the   been not identified.
              products’expiration date, Moreover, topical      This study will determine the bioactivity
              applications of the herb have been used to   of T. triquetrum extract in dosages and formu-

              treat infectious wounds. [1,4,7]            lations found in Thai folk medicine practice.
                   Researchers have also found this herb    Specifically, we aim to investigate the herb’s

              useful in food preservation and has effects   antioxidant activity, anti-microbial activity,
              on different types of cells. Researchers found   and the inhibition activity of the PDE-5 en-
              that maggot growth in pickled fish was limited   zyme.

              when T. triquetrum extract was incorporated
              as an ingredient. Moreover, bacterial growth             Methodology
              was suppressed, so the food expiration date   Materials

              was extended.  Recently, researchers revealed      Tadehagi triquetrum (L.) H. Ohashi leaves
                          [9]
              evidence that T. triquetrum contained ligand   were collected and identified by a botanist of
              and phenylated isoflavones that prevented   Tungsleangluang National Park, Petchaboon

              cell activity. They inhibited effects of chemi-  Province, Thailand. DPPH (1,1-diphenyl-
              cal toxicity on hepatocyte cells by decreasing   2-picrylhydrazyl), ABTS (2,2′-azino-bis-3-eth-

              the expression of NFκB.  Tadehaginosin and   ylbenzthiazoline-6-sulphonic acid), MTT ([3-(4,
                                   [10]
              3,4-dihydro-4-(40-hydroxyphenyl)-5,7-dihy-  5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-]
              droxycoumarin were isolated from the aerial   tetrazolium bromide) and 95% ethanol were

              part of T. triquetrum. These two compounds   AR grade and purchased from Sigma (Sigma-
              displayed hypoglycemic activity by increas-  Aldrich, Singapore)

              ing glucose consumption and reducing blood
              glucose levels.  Phenylpropanoid glucosides   Instruments
                           [11]
              and tadehaginosides increased glucose uptake      - UV-Visible spectrophotometer (Evolu-

              by inducing peroxisome proliferator-activated   tion 201, Thermo Scientific)
              receptor γ (PPARγ) activity and glucose trans-     - Microplate reader (Eon, Biotek)
              porter-4 (GLUT-4) expression.  The alterna-
                                        [12]
   34   35   36   37   38   39   40   41   42   43   44