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วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก Journal of Thai Traditional & Alternative Medicine
ปีที่ 18 ฉบับที่ 2 พฤษภาคม-สิงหาคม 2563 Vol. 18 No. 2 May-August 2020

นิพนธ์ต้นฉบับ

Bioactivity Study of Tadehagi triquetrum Extracts Used in Thai

Folk Medicine

Wudtichai Wisuitiprot *,†,¶ , Vanuchawan Wisuitiprot , Nipon Kaewtai , Padet Sreenoulkow §
*
‡
* Department of Thai Traditional Medicine, Sirindhorn College of Public Health, Phitsanulok 65130, Thailand
† Herbal Research Unit, Sirindhorn College of Public Health, Phitsanulok 65130, Thailand
‡ Cosmetic and Natural Product Research Center, Faculty of Pharmaceutical Science, Naresuan University, Phitsanulok 65000,
Thailand
§ Chaloem Phrakiat 60 Phansa Nawamintha Rachini Health Center, Pa Bon District, Phatthalung 93170, Thailand
¶ Corresponding author: wudtichai@scphpl.ac.th

Abstract
Tadehagi triquetrum (L.) H.Ohashi (tankhoma in Thai) is a medicinal plant that has been traditionally used as
an antimicrobial agent for extending the expiration date of products containing water as vehicle. Thai folk medicine
practitioners have also used a decoction of T. triquetrum leaves for improving physical and sexual performances
in men. The aim of our study was to determine the bioactivity of T. triquetrum extracts using different polar and
nonpolar solvents. We evaluated the antioxidant activity of extracts using DPPH and ABTS assays, investigated
phosphodiesterase-5 (PDE-5) inhibitory activity of the extracts, which is associated with enhancing sexual and
physical performance, and measured antimicrobial activity of the extracts using disc diffusion and minimal inhibi-
tory concentration (MIC). The results revealed that the ethanolic and water extracts showed potent antioxidant
and antimicrobial activities. Water extract presented the highest antioxidant activity (IC50 = 12.45 ± 0.14 mg/
mL). Polar extracts inhibited the growth of microorganisms S. aureus, E. coli and P. aeruginosa. The non-polar
extract effectively suppressed fungal growth. Interestingly, the Thai folk medicine formulation of T. triquetrum
water extract at 50 mg/mL inhibited PDE-5 activity by 60.45% (SD = 0.14). This level of inhibitory bioactivity is
considered good. As these findings are promising, future in vitro, in vivo, and clinical studies should be conducted
to examine properties of this plant in greater detail.
Key words: antimicrobial activity, PDE-5 inhibition, antioxidant activity, Tadehagi triquetrum

Received date 18/11/19; Revised date 22/07/20; Accepted date 21/08/20

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