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วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก  Journal of Thai Traditional & Alternative Medicine
                        ปีที่ 18  ฉบับที่ 2  พฤษภาคม-สิงหาคม 2563       Vol. 18  No. 2  May-August 2020




                                                                                   นิพนธ์ต้นฉบับ



              Bioactivity Study of Tadehagi triquetrum Extracts Used in Thai

              Folk Medicine



              Wudtichai Wisuitiprot *,†,¶ , Vanuchawan Wisuitiprot , Nipon Kaewtai , Padet Sreenoulkow §
                                                                    *
                                                       ‡
              * Department of Thai Traditional Medicine, Sirindhorn College of Public Health, Phitsanulok 65130, Thailand
              † Herbal Research Unit, Sirindhorn College of Public Health, Phitsanulok 65130, Thailand
              ‡ Cosmetic and Natural Product Research Center, Faculty of Pharmaceutical Science, Naresuan University, Phitsanulok 65000,
              Thailand
              § Chaloem Phrakiat 60 Phansa Nawamintha Rachini Health Center, Pa Bon District, Phatthalung 93170, Thailand
              ¶ Corresponding author: wudtichai@scphpl.ac.th










                                                   Abstract
                      Tadehagi triquetrum (L.) H.Ohashi (tankhoma in Thai) is a medicinal plant that has been traditionally used as
                 an antimicrobial agent for extending the expiration date of products containing water as vehicle. Thai folk medicine
                 practitioners have also used a decoction of T. triquetrum leaves for improving physical and sexual performances
                 in men. The aim of our study was to determine the bioactivity of T. triquetrum extracts using different polar and
                 nonpolar solvents. We evaluated the antioxidant activity of extracts using DPPH and ABTS assays, investigated
                 phosphodiesterase-5 (PDE-5) inhibitory activity of the extracts, which is associated with enhancing sexual and
                 physical performance, and measured antimicrobial activity of the extracts using disc diffusion and minimal inhibi-
                 tory concentration (MIC). The results revealed that the ethanolic and water extracts showed potent antioxidant
                 and antimicrobial activities. Water extract presented the highest antioxidant activity (IC50 = 12.45 ± 0.14 mg/
                 mL). Polar extracts inhibited the growth of microorganisms S. aureus, E. coli and P. aeruginosa. The non-polar
                 extract effectively suppressed fungal growth. Interestingly, the Thai folk medicine formulation of T. triquetrum
                 water extract at 50 mg/mL inhibited PDE-5 activity by 60.45% (SD = 0.14). This level of inhibitory bioactivity is
                 considered good. As these findings are promising, future in vitro, in vivo, and clinical studies should be conducted
                 to examine properties of this plant in greater detail.
                      Key words:  antimicrobial activity, PDE-5 inhibition, antioxidant activity, Tadehagi triquetrum







              Received date 18/11/19; Revised date 22/07/20; Accepted date 21/08/20

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