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266 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก     ปีที่ 18  ฉบับที่ 2  พฤษภาคม-สิงหาคม 2563




             Table 1 Antioxidant activity of T. triquetrum extracts determined by DPPH and ABTS assays

                                                                 Antioxidant activities
             Extracts
                                                   IC50 (µg/mL) ± SD             TEAC (µM) ± SD

             95% ethanol                             123.60 ± 2.78                 1.18 ± 0.01
             50% ethanol                             66.14 ± 2.57                  1.20 ± 0.04
             Hexane                                  180.20 ± 1.79                 5.50 ± 0.86
             Water                                   12.45 ± 0.14                  0.85 ± 0.11
             Trolox                                   0.89 ± 0.04                      -



             Table 2  PDE-5 inhibitory activity of T. triquetrum   measurements indicated that the extract with
                    extracts                             a particular solvent extract was more effective

             Extracts       %Inhibitory activity at 50 µg/mL*  in stopping microbial growth. Ethanolic and
                                                         water extracts showed obvious inhibition ef-
             95% ethanol           39.86 ± 3.20
             50% ethanol           52.53 ± 2.60          fect on bacterial growth with moderate clear
             Hexane                11.75 ± 1.98          zone measurements for bacteria. Hexane
             Water                 60.45 ± 0.14          extract presented growth inhibition of yeast

             *Data are reported as the mean ± S.D and are derived   with moderate clear zone measurements for
             from three repeats.
                                                         yeast.
                                                              The minimal inhibitory concentration

             activity (60.45% ± 0.14). Hexane extract of T.   (MIC) of each extract shown in Table 4 corre-
             triquetrum showed the lowest inhibition of   lated with the clear zone measurement results

             PDE-5 activity (11.75% ± 1.98).             presented in Table 3. The MIC is the smallest
                 4.  Antimicrobial screening             concentration of the particular extract that
                   Antimicrobial activity was determined   was necessary to inhibit microbial growth. The

             by using disc diffusion and broth dilution as-  water extract had the lowest MIC compared
             says. We examined the effects of T. triquetrum   to other polar extracts. This indicates that
             on various microorganisms including E. coli,   the water extract has the highest antibacte-

             S. aureus, Ps. aeruginosa and C. albicans.   rial activity of the different types of solvent
                 Table 3 shows that inhibitory zone, or   because only a relatively low concentration
             clear zone measurements (mm) for cell cul-  was necessary to inhibit bacterial growth.

             tures treated with T. triquetrum extracts in   Interestingly, only hexane extract shows the
             different solvents varied. Larger clear zone   inhibition activity of yeast (C. Albicans).
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