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266 วารสารการแพทย์แผนไทยและการแพทย์ ทางเลือก ปีที่ 18 ฉบับที่ 2 พฤษภาคม-สิงหาคม 2563

Table 1 Antioxidant activity of T. triquetrum extracts determined by DPPH and ABTS assays

Antioxidant activities
Extracts
IC50 (µg/mL) ± SD TEAC (µM) ± SD

95% ethanol 123.60 ± 2.78 1.18 ± 0.01
50% ethanol 66.14 ± 2.57 1.20 ± 0.04
Hexane 180.20 ± 1.79 5.50 ± 0.86
Water 12.45 ± 0.14 0.85 ± 0.11
Trolox 0.89 ± 0.04 -

Table 2 PDE-5 inhibitory activity of T. triquetrum measurements indicated that the extract with
extracts a particular solvent extract was more effective

Extracts %Inhibitory activity at 50 µg/mL* in stopping microbial growth. Ethanolic and
water extracts showed obvious inhibition ef-
95% ethanol 39.86 ± 3.20
50% ethanol 52.53 ± 2.60 fect on bacterial growth with moderate clear
Hexane 11.75 ± 1.98 zone measurements for bacteria. Hexane
Water 60.45 ± 0.14 extract presented growth inhibition of yeast

*Data are reported as the mean ± S.D and are derived with moderate clear zone measurements for
from three repeats.
yeast.
The minimal inhibitory concentration

activity (60.45% ± 0.14). Hexane extract of T. (MIC) of each extract shown in Table 4 corre-
triquetrum showed the lowest inhibition of lated with the clear zone measurement results

PDE-5 activity (11.75% ± 1.98). presented in Table 3. The MIC is the smallest
4. Antimicrobial screening concentration of the particular extract that
Antimicrobial activity was determined was necessary to inhibit microbial growth. The

by using disc diffusion and broth dilution as- water extract had the lowest MIC compared
says. We examined the effects of T. triquetrum to other polar extracts. This indicates that
on various microorganisms including E. coli, the water extract has the highest antibacte-

S. aureus, Ps. aeruginosa and C. albicans. rial activity of the different types of solvent
Table 3 shows that inhibitory zone, or because only a relatively low concentration
clear zone measurements (mm) for cell cul- was necessary to inhibit bacterial growth.

tures treated with T. triquetrum extracts in Interestingly, only hexane extract shows the
different solvents varied. Larger clear zone inhibition activity of yeast (C. Albicans).