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√Ÿª∑’Ë 1 A-C · ¥ß TLC Chromatogram ¢Õß “√ °—¥À≠⓬ÿà¡ · ¥ß°“√·¬° “√°≈ÿà¡¡’¢—È« (polar compounds) “√
°—¥À≠⓬ÿࡇ¢â¡¢âπ 1 mg/ml “√¡“μ√∞“π‡¢â¡¢âπ 10 mg/ml À¬¥ª√‘¡“μ√ 5 ‰¡‚§√≈‘μ√ «—Ø¿“§‡§≈◊ËÕπ∑’˧◊Õ
ethyl acetate:methanol:formic acid:water (50:3:3:6) ≈”¥—∫°“√À¬¥ “√μ“¡À¡“¬‡≈¢ ¥—ßπ’È 1 “√ °—¥
hexane, 2 “√ °—¥ chloroform, 3 “√ °—¥ 95% ethanol, 4 “√ °—¥ acetone, 5 “√ °—¥πÈ”, 6 “√
¡“μ√∞“π rutin, 7 “√¡“μ√∞“π methyl salicylate, 8 “√¡“μ√∞“π gallic acid
√Ÿª∑’Ë 2 A-C · ¥ß TLC Chromatogram ¢Õß “√ °—¥À≠⓬ÿà¡ · ¥ß°“√·¬° “√°≈ÿࡉ¡à¡’¢—È« (non-polar compounds)
“√ °—¥À≠⓬ÿࡇ¢â¡¢âπ 1 mg/ml “√¡“μ√∞“π‡¢â¡¢âπ 10 mg/ml À¬¥ª√‘¡“μ√ 5 ‰¡‚§√≈‘μ√ «—Ø¿“§
‡§≈◊ËÕπ∑’˧◊Õ chloroform:methanol (9:1) ≈”¥—∫°“√À¬¥ “√μ“¡À¡“¬‡≈¢ ¥—ßπ’È 1 “√ °—¥ hexane, 2 “√ °—¥
chloroform, 3 “√ °—¥ 95% ethanol, 4 “√ °—¥ acetone, 5 “√ °—¥πÈ”, 6 “√¡“μ√∞“π rutin, 7 “√
¡“μ√∞“π piperine 8 “√¡“μ√∞“π gallic acid
4. °“√«‘‡§√“–ÀåÕߧåª√–°Õ∫∑“߇§¡’¢Õß “√ 5 μ“¡≈”¥—∫ Õߧåª√–°Õ∫∑“߇§¡’∑’Ëμ√«®æ∫„π “√
°—¥¥â«¬ GC-MS °—¥À¬“∫ acetone ¥—ß· ¥ß„πμ“√“ß∑’Ë 3 æ∫«à“
“√μà“ß Ê ∑’Ëμ√«®æ∫„π “√ °—¥À¬“∫∑’Ë °—¥ ª√–°Õ∫‰ª¥â«¬ “√°≈ÿࡉ¡à¡’¢—È« ‰¥â·°à “√°≈ÿà¡ phy-
®“°μ—«∑”≈–≈“¬ acetone, §«—π ( à«π∑’Ë≈–≈“¬πÈ”) tosterols [‡™àπ stigmasterol, β-sitosterol] “√°≈ÿà¡
∑’ˉ¥â®“°°“√‡º“À≠⓬ÿà¡ (grass smoke) ·≈– à«π∑’Ë °√¥‰¢¡—π [‡™àπ palmitic acid] ·≈– “√ α-toco-
‡ªìπ§√“∫‡À≈◊Õß (smoke residue) · ¥ßμ“√“ß∑’Ë 3- pherol ‡ªìπμâπ πÕ°®“°π’Ȭ—ߪ√–°Õ∫¥â«¬ “√∑’ˉ¡à
