Page 29 - วารสารปีที่15ฉบับที่1

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√Ÿª∑’Ë 7 Ÿμ√‚§√ß √â“ߢÕß “√∫√‘ ÿ∑∏‘Ï∑’Ë·¬°‰¥â®“°
°√–∫«π°“√‚§√¡“‚∑°√“øï

√Ÿª∑’Ë 6 Ÿμ√‚§√ß √â“ß∑“߇§¡’¢Õß “√∑’Ëæ∫„𧫗𮓰
°“√‡º“À≠⓬ÿà¡

[22]
°“√μ’æ‘¡æå·≈â« πÕ°®“°π’Èæ∫ “√ 4-coumaric
1
acid ‚¥¬¡’¢âÕ¡Ÿ≈ H NMR (400 MHz) ¢Õß “√ 4-
1
coumaric acid Õ¥§≈âÕß°—∫¢âÕ¡Ÿ≈ H NMR ¢Õß
[23]
“√¡“μ√∞“π·≈–√“¬ß“π«‘®—¬∑’ˉ¥â¡’°“√μ’æ‘¡æå·≈â«
5,7,4'-trimethoxyflavone (C H O ; MW
18 16 5
1
312) H NMR data (400 MHz; CD OD): δ 7.95
3 √Ÿª∑’Ë 8 °√“ø¡“μ√∞“π· ¥ß§«“¡ —¡æ—π∏å√–À«à“ߧà“
(2H, d, J = 9.2 Hz, H-2' and H-6'), 7.10 (2H, d, °“√¥Ÿ¥°≈◊π·≈–§«“¡‡¢â¡¢âπ¢Õß gallic acid
J = 8.9 Hz, H-3' and H-5'), 6.81 (1H, d, J = 1.9

Hz, H-8), 6.63 (1H, s, H-3), 6.59 (1H, d, J = 1.9
Hz, H-6), 3.95 (3H, s, OCH -5), 3.92 (3H, s,
3
OCH -7), 3.89 (3H, s, OCH -4')
3 3
1
4-coumaric acid (C H O ; MW 164) H
9 8 3
NMR data (400 MHz; CD OD): δ 7.57 (1H, d, J
3
= 15.9 Hz, H-7), 7.43 (2H, d, J = 8.6 Hz, H-2
and H-6), 6.76 (2H, d, J = 8.6 Hz, H-3 and H-
√Ÿª∑’Ë 9 °√“ø¡“μ√∞“π· ¥ß§«“¡ —¡æ—π∏å√–À«à“ߧà“
5), 6.26 (1H, d, J = 15.8 Hz, H-8)
°“√¥Ÿ¥°≈◊π·≈–§«“¡‡¢â¡¢âπ¢Õß quercetin
6. °“√À“ª√‘¡“≥øïπÕ≈‘°√«¡ ·≈–ª√‘¡“≥
‡ø≈‚«πÕ¬¥å√«¡ Ÿß ÿ¥μàÕπÈ”Àπ—°À≠â“·Àâß ¡’§à“‡∑à“°—∫ 1770.49 μg
6.1 °“√À“ª√‘¡“≥øïπÕ≈‘°√«¡ GAE μàÕ “√ °—¥·Àâß 1 °√—¡ ¥—ß· ¥ß„πμ“√“ß∑’Ë 6

°“√À“ ¡°“√‡™‘߇ âπ®“° “√¡“μ√∞“π gal- 6.2 °“√À“ª√‘¡“≥‡ø≈‚«πÕ¬¥å√«¡
lic acid ¡’§à“‡∑à“°—∫ y = 0.0102x - 0.0968 ‚¥¬¡’ °“√À“ ¡°“√‡™‘߇ âπ®“° “√¡“μ√∞“π
2
R = 0.9816 ‡¡◊ËÕ«‘‡§√“–Àå “√ °—¥·≈–π”¡“§”π«≥ quercetin ¡’§à“‡∑à“°—∫ y = 0.0428x + 0.0132 ‚¥¬
2
ª√‘¡“≥øïπÕ≈‘°√«¡ æ∫«à“ “√ °—¥πÈ”μâ¡¡’ª√‘¡“≥ ¡’ R = 0.9959 ·≈–ª√‘¡“≥‡ø≈‚«πÕ¬¥å√«¡μàÕπÈ”