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ª√–‡∑»æ∫„π·Õø√‘°“ Õ‘π‡¥’¬ ¿Ÿ¡‘¿“§Õ‘π‚¥®’π ‘ßÀ“§¡ æ.». 2557 - ‡¥◊Õπ°—𬓬π æ.». 2558

¡“‡≈‡´’¬ ÕÕ ‡μ√‡≈’¬ ·≈–À¡Ÿà‡°“–„π¡À“ ¡ÿ∑√ 1.2 “√‡§¡’·≈–μ—«∑”≈–≈“¬
[5,6]
·ª´‘øî° À≠⓬ÿà¡À√◊ÕÀ≠â“À’¬ÿࡇªìπ™◊ËÕ‡√’¬°¢Õß methanol HPLC, ethanol AR, acetone
À≠â“μ“¡ √√æ§ÿ≥æ◊Èπ∫â“π¢Õß°“√„™â„πºŸâÀ≠‘ß AR, hexane AR, chloroform AR, ethyl acetate

À≈—ߧ≈Õ¥„π¿“§μ–«—πÕÕ°‡©’¬ß‡Àπ◊Õ‡æ◊ËÕ„Àâ¡¥≈Ÿ° AR, deionized water, deuterated-methanol,
°√–™—∫ √—°…“·º≈ ‚¥¬π”¡“μâ¡πÈ”‡æ◊ËÕ¥◊Ë¡ ”À√—∫ anisaldehyde, sulfuric acid, formic acid, gla-
∫”√ÿß√à“ß°“¬ À√◊Õπ”¡“ ÿ¡‰ø„À⇰‘¥§«—π‡æ◊ËÕ√¡ cial acetic acid, bismuth oxynitrate, potas-
[1]
∫“¥·º≈„Àâ·Àâ߇√Á«¥â«¬°“√¬◊π§√àÕ¡À√◊Õπ—Ëß∂à“𠧔 sium iodide, diphenylboric acid-2-aminoethyl
«à“ ¬ÿà¡ ‡ªìπ§”„π¿“…“Õ’ “π À¡“¬∂÷ß √—¥‡¢â“ ∫’∫ ester, 4-coumaric acid, gallic acid, piperine,
[1]
√«∫‡¢â“‰«â¥â«¬°—π ∑”„Àâ·πàπ¢÷Èπ ™“«æ◊Èπ‡¡◊Õß„πÀ¡Ÿà rutin, methyl salicylate, quercetin, Folin-
‡°“–‚√∑Ÿ¡—π„π¡À“ ¡ÿ∑√·ª´‘øî°„™â„∫¢Õß C. Ciocalteuûs phenol reagent, sodium carbon-
[7]
lappacea (L.) Desv. ”À√—∫‚√§μ‘¥‡™◊ÈÕ πÈ”μâ¡¢Õß ate, aluminium chloride, vitamin A (β-caro-
C. lappacea (L.) Desv. ”À√—∫Õ“°“√‡≈◊Õ¥‰À≈ÕÕ° tene), vitamin B , vitamin B , vitamin C, vi-
1 2
[8]
∑“ß™àÕߧ≈Õ¥ ·≈–¡’°“√„™âª√–‚¬™π凪ìπÕ“À“√ tamin E (α-tocopherol), alanine, arginine, as-
[4]
—μ«å °“√»÷°…“π’È¡’«—μ∂ÿª√– ߧå‡æ◊ËÕ»÷°…“Õߧå partic acid, cysteine, glutamic acid, glycine,
ª√–°Õ∫∑“߇§¡’®“° à«π‡Àπ◊Õ¥‘π¢ÕßÀ≠⓬ÿà¡ (C. histidine, isoleucine, leucine, lysine, methion-
lappacea (L.) Desv.) ‚¥¬‡∑§π‘§μà“ß Ê ·≈–‡æ◊ËÕ ine, phenylalanine, proline, serine, threonine,

√«∫√«¡Õߧ姫“¡√Ÿâ‡°’ˬ«°—∫Õߧåª√–°Õ∫∑“߇§¡’ tryptophan, tyrosine, valine ·≈– Sephadex LH-
¢ÕßÀ≠⓬ÿà¡ ”À√—∫𔉪»÷°…“‡æ‘Ë¡‡μ‘¡∑“ßæ√’ 20 ( “√‡§¡’„π°“√∑¥≈Õ߇ªìπ™π‘¥∑’Ë„™â°—∫ß“π
§≈‘π‘°·≈–∑“ߧ≈‘π‘°μàÕ‰ª «‘‡§√“–Àå ¬°‡«âπ√–∫ÿ‡ªìπ™π‘¥Õ◊Ëπ) °“√‡μ√’¬¡ reagent

μà“ß Ê μ“¡«‘∏’∑’Ë√–∫ÿ‰«â„π Thai Herbal Pharmaco-
[9]
√–‡∫’¬∫«‘∏’»÷°…“ poeia “√ °—¥‡¢â¡¢âπ 1 mg/ml ·≈– “√¡“μ√∞“π
‡¢â¡¢âπ 10 mg/ml ≈–≈“¬„π methanol
1. «— ¥ÿ
1.3 ‡§√◊ËÕß¡◊Õ
1.1 μ—«Õ¬à“ßÀ≠⓬ÿà¡ ™ÿ¥‡§√◊ËÕß¡◊Õ TLC (Camag Linomat 5 TLC

μ—«Õ¬à“ßÀ≠⓬ÿà¡ ( à«π‡Àπ◊Õ¥‘π) ∑’Ë·∑â®√‘߉¥â√—∫ Automatic Sampler; Camag TLC Visualizer
°“√æ‘ Ÿ®πå‡Õ°≈—°…≥å ®“°‚√ß欓∫“≈‡®â“æ√–¬“ ·≈– Camag TLC Scanner 3), ‡§√◊ËÕß GC-MS
Õ¿—¬¿Ÿ‡∫»√ ‰¥â√—∫√–À«à“߇¥◊Õπ ‘ßÀ“§¡ æ.». 2557 √ÿàπ MS Thermo Polaris Q, with NIST M.S.

®“°π—Èππ”¡“Õ∫®π·Àâß ∑’ËÕÿ≥À¿Ÿ¡‘‰¡à‡°‘π 40 Õß»“ search program V 2.0 (2005) ·≈– ‡§√◊ËÕß UV/
‡´≈‡´’¬ ‡ªìπ‡«≈“ 3 «—π ∫¥‡ªìπºß≈–‡Õ’¬¥ ºà“π Vis Spectrophotometer (Labtech UV2000) ≥

·√à߇∫Õ√å 80 ‡°Á∫„π¿“™π–ªî¥ π‘∑ªÑÕß°—𧫓¡™◊Èπ °√¡°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ°
√–¬–‡«≈“„π°“√¥”‡π‘π°“√»÷°…“√–À«à“߇¥◊Õ𠇧√◊ËÕß HPLC (Agilent) ≥ ∂“∫—π§âπ§«â“·≈–