Page 14 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 10 ฉบับที่ 3

164 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë 10 ©∫—∫∑’Ë 3°—𬓬π-∏—𫓧¡ 2555

‡Õπ‰´¡åπ’È®÷߇ªìπ∑’Ë π„®¢Õßπ—°«‘∑¬“»“ μ√å„πÀ≈“¬

°≈ÿà¡ ¡’ “√®“°æ◊™ ¡ÿπ‰æ√À≈“¬™π‘¥ (μ“√“ß∑’Ë 1)
∑’Ë¡’§«“¡ “¡“√∂„π°“√¬—∫¬—Èß beta-lactamases ‡™àπ
epigallocatechin gallate (EGCg) ·¬°‰¥â®“°™“

(Camellia sinensis) “¡“√∂¬—∫¬—Èß°“√º≈‘μ peni-
cillinase ®“° MRSA ·≈– beta-lactamase pro-
ducing S. aureus à߇ √‘¡°“√∑”ß“π¢Õß penicil-
lin ·≈– ampicillin/sulbactam ‡¡◊ËÕ∑¥ Õ∫‚¥¬«‘∏’

checkerboard assay ·≈– time kill assay æ∫«à“
EGCg ´÷Ëß¡’§à“MIC ‡ªìπ 100 mg/L ∑’˧«“¡‡¢â¡¢âπ

μË”°«à“§à“ MIC (sub-MIC) “¡“√∂≈¥§à“ MIC
¢Õ߬“¥—ß°≈à“«μàÕ‡™◊ÈÕ∑¥ Õ∫≈߉¥â√–À«à“ß 4-16
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‡∑à“ ¢à“‡≈Á° (Alpinia officinarum) ‡ªìπæ◊™ ¡ÿπ
‰æ√‰∑¬Õ’°™π‘¥Àπ÷Ëß∑’Ë¡’§«“¡ “¡“√∂„π°“√≈¥°“√

¥◊ÈÕ¬“¢Õß S. aureus æ∫«à“ galangin “√∫√‘ ÿ∑∏‘Ï∑’Ë
·¬°‰¥â®“°¢à“‡≈Á°‡æ‘Ë¡§«“¡ “¡“√∂„π°“√¬—∫¬—Èß

·∫§∑’‡√’¬¢Õ߬“‰¥âÀ≈“¬™π‘¥ ‡™àπ amoxicillin,
ampicillin, penicillin G, ·≈– cloxacillin ‡ªìπμâπ
‡¡◊ËÕ∑¥ Õ∫¥â«¬«‘∏’ checkerboard assay §à“ FIC
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index ¡’§à“√–À«à“ß <0.02-0.11 ®“°°“√∑¥ Õ∫
√Ÿª∑’Ë 1 °“√ª√–‡¡‘πº≈°“√∑¥ Õ∫ªØ‘°‘√‘¬“√–À«à“ß
eugenol ´÷Ë߇ªìπ “√ ”§—≠¢ÕßπÈ”¡—π°“πæ≈Ÿ
“√≈¥°“√¥◊ÈÕ¬“·≈–¬“¥â«¬«‘∏’ (°) time kill
assay · ¥ßº≈°“√‡ √‘¡ƒ∑∏‘Ï√–À«à“ß “√≈¥ (Eugenia aromatica) ¥â«¬«‘∏’ isobole method
23
°“√ ¥◊ÈÕ¬“ ellagic acid ·≈–¬“ rifampicin , æ∫«à“ “√¥—ß°≈à“« “¡“√∂‡ √‘¡ƒ∑∏‘Ï°—∫ ampicillin
22
(¢) Epsilometer test , ·≈– (§) isobole
„π°“√¬—∫¬—Èß°“√‡®√‘≠¢Õß·∫§∑’‡√’¬À≈“¬™π‘¥ ‡™àπ
24
method
E. coli, P. aeruginosa, E. aerogenes, S.
9
Typhimurium ·≈– Proteus vulgaris πÕ°®“°
“√≈¥°“√¥◊ÈÕ¬“∑’Ë “¡“√∂®—∫°—∫‡Õπ‰´¡å beta- π’È®“°°“√»÷°…“§«“¡ “¡“√∂„π°“√¬—∫¬—È߇Õπ‰´¡å
lactamases ¬—∫¬—Èß°“√ hydrolysis ¬“ªØ‘™’«π– ¡’ beta-lactamases „πÀ≈Õ¥∑¥≈ÕߢÕßæ◊™ ¡ÿπ

°“√π”¡“„™â∑“ß°“√§â“√à«¡°—∫ amoxicillin ·μà°“√ ‰æ√®”π«π 16 ™π‘¥„π·§¡‡¡Õ√Ÿπ (Cameroon)
¥◊ÈÕμàÕ clavulanic acid ¢Õ߇Õπ‰´¡å„π°≈ÿà¡π’Ȭ—ß æ∫«à“¡“°°«à“ 90% ¢Õß ¡ÿπ‰æ√∑’Ë∑¥ Õ∫ “¡“√∂
§ß¡’√“¬ß“πÕ¬Ÿà∑—Ë«‰ª °“√À“ “√≈¥°“√¥◊ÈÕ¬“∑“ß ¬—∫¬—È߇Õπ‰´¡åπ’ȉ¥âÕ¬à“ßπâÕ¬ 1 ™π‘¥ Õ¬à“߉√°Áμ“¡
28
‡≈◊Õ°®“°·À≈àß„À¡à‡æ◊ËÕ„™â„π°“√¬—∫¬—ÈßÀ√◊Õ∑”≈“¬ º≈°“√∑¥≈Õßπ’Ȭ—߉¡à‰¥â∑¥ Õ∫„π·∫§∑’‡√’¬ ®“°