Page 45 - journal-9-2-Full

122 «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë 9©∫—∫∑’Ë 2°—𬓬π-∏—𫓧¡ 2554

Abstract
DNA Fingerprinting of Derris scandens Benth. from Callus and Different Locations
Thanyawan Mongkolchaipak*, Sorrapetch Marsud*, Paparvadee Suchantaboot*
*Medicinal Plant Research Institute, Department of Medical Sciences, Ministry of Public Health,
Nonthaburi Province
Twenty samples of Derris scandens Benth., 19 of which were collected from different provinces and one
which was from a callus cultured on media formulation 43+C1, underwent morphological study, DNA finger-
print analysis, and TLC fingerprint analysis. Morphological study of the 19 samples showed no difference in
characteristics except for the diameter of the stems; the largest was the sample from Phitsanulok (6 cm) and
the smallest was the sample from Lop Buri (1.7 cm). However, they were in the same subgroup 2.2, which
indicated that the diameter of stems may depend on the age, climate, zone, water and soil where the plant
originates.
Analysis of the DNA fingerprint of D. scandens by Winboot program indicated that it was divided into 3
groups. Group 1 was further divided into subgroups 1.1 and 1.2. Those in subgroup 1.1 were from Phetchaburi
(Khaokrapook), Prachuap Kiri Khan (Saovapha), Sa Kaeo, Nakhon Pathom (SL) and callus. Those in sub-
groups 1.2 were from Nonthaburi, Rayong, Chanthaburi. Group 2 was further divided into subgroups 2.1 and
2.2. Those in subgroup 2.1 were from Khon Kean, Prachuap Kiri Khan (Ao Noi), Nakhon Ratchasima,
Phetchaburi (Kladlaung) and Nakhon Nayok. Those in subgroup 2.2 were from Kanchanaburi (Bunwangyen),
Phitsanulok, Lop Buri, Nakhon Pathom (KU), Buri Ram and Bangkok. The one in group 3 was from
Prachinburi only. TLC fingerprint identification showed that the TLC fingerprint pattern of all samples was
similar. The present study gave preliminary results on genetic differences of D. scandens Benth. that could be
used to identify different varieties of D. scandens and for future genetic development of the plant.
Key words: Derris scandens, DNA fingerprinting, TLC fingerprint analysis