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                  Abstract
                       DNA Fingerprinting of Derris scandens Benth. from Callus and Different Locations
                       Thanyawan Mongkolchaipak*, Sorrapetch Marsud*, Paparvadee Suchantaboot*
                       *Medicinal Plant Research Institute, Department of Medical Sciences, Ministry of Public Health,
                       Nonthaburi Province
                           Twenty samples of Derris scandens Benth., 19 of which were collected from different provinces and one
                       which was from a callus cultured on media formulation 43+C1, underwent morphological study, DNA finger-
                       print analysis, and TLC fingerprint analysis.  Morphological study of the 19 samples showed no difference in
                       characteristics except for the diameter of the stems; the largest was the sample from Phitsanulok (6 cm) and
                       the smallest was the sample from Lop Buri (1.7 cm).  However, they were in the same subgroup 2.2, which
                       indicated that the diameter of stems may depend on the age, climate, zone, water and soil where the plant
                       originates.
                           Analysis of the DNA fingerprint of D. scandens by Winboot program indicated that it was divided into 3
                       groups.  Group 1 was further divided into subgroups 1.1 and 1.2.  Those in subgroup 1.1 were from Phetchaburi
                       (Khaokrapook), Prachuap Kiri Khan (Saovapha), Sa Kaeo, Nakhon Pathom (SL) and callus.  Those in sub-
                       groups 1.2 were from Nonthaburi, Rayong, Chanthaburi. Group 2 was further divided into subgroups 2.1 and
                       2.2. Those in subgroup 2.1 were from Khon Kean, Prachuap Kiri Khan (Ao Noi), Nakhon Ratchasima,
                       Phetchaburi (Kladlaung) and Nakhon Nayok.  Those in subgroup 2.2 were from Kanchanaburi (Bunwangyen),
                       Phitsanulok, Lop Buri, Nakhon Pathom (KU), Buri Ram and Bangkok.  The one in group 3 was from
                       Prachinburi only.   TLC fingerprint identification showed that the TLC fingerprint pattern of all samples was
                       similar.  The present study gave preliminary results on genetic differences of D. scandens Benth. that could be
                       used to identify different varieties of D. scandens and for future genetic development of the plant.
                       Key words:  Derris scandens, DNA fingerprinting, TLC fingerprint analysis
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