Page 42 - journal-9-2-Full

Journal of Thai Traditional & Alternative Medicine Vol. 9 No. 2 September-December 2011 119

16
+ PhetburiKKP ‰¥â πÕ°®“°π—Èπ¬—ß “¡“√∂·¬°§«“¡·μ°μà“ߢÕßæ◊™ ¡ÿπ
+ 72.0
‰æ√ °ÿ≈ Derris 5 ™π‘¥ ‰¥â·°à D. scandens (‡∂“«—≈¬å‡ª√’¬ß),
| + PrachuapSao
D. elliptica (À“߉À≈·¥ß), D. malaccensis (À“߉À≈¢“«),
+-14.6
+ Sarkaew
D. trifoliata (∂Õ∫·∂∫πÈ”) ·≈– D. reticulate (™–‡Õ¡‡Àπ◊Õ)
1.1
+ 40.3
‚¥¬„™â‡∑§π‘§ Random amplification of polymorphic
+-14.1 + Nakornpatom (SL)
+-12.8 |
DNA RAPD “¡“√∂·∫àß°≈ÿà¡æ◊™ ¡ÿπ‰æ√‰¥â 2 °≈ÿà¡ ´÷Ëß D.
+ Callus
trifoliata ·¬°§«“¡·μ°μà“ߥâ“πæ—π∏ÿ°√√¡®“°æ◊™ ¡ÿπ‰æ√∑—Èß
+ Nontaburi
17
4 ™π‘¥ ‰¥â
+ 33.1
Õ¬à“߉√°Áμ“¡º≈°“√»÷°…“≈“¬æ‘¡æ奒‡ÕÁπ‡Õ¢Õ߇∂“«—≈¬å
| + Rayong
1.2
+-76.2
‡ª√’¬ß„π√“¬ß“ππ’È ∂◊Õ‰¥â«à“‡ªìπ¢âÕ¡Ÿ≈‡∫◊ÈÕßμâπ„π°“√«‘®—¬
+ Chantaburi
‡°’ˬ«°—∫¢âÕ¡Ÿ≈æ◊Èπ∞“π∑“ßæ—π∏ÿ°√√¡¢Õ߇∂“«—≈¬å‡ª√’¬ß ∑’Ë®–
+-20.4 + Konkaen
𔉪 Ÿà§«“¡‡™◊ËÕ¡‚¬ß¢Õßæ—π∏ÿ°√√¡°—∫°“√ √â“ß “√ ”§—≠∑’Ë¡’
+-34.9
+-28.0 + PrachuapAnoi
§ÿ≥ ¡∫—쑇ªì𬓠¡ÿπ‰æ√„π‡∂“«—≈¬å‡ª√’¬ß √«¡∂÷ߧÿ≥ ¡∫—μ‘

¥â“𰓬¿“æÕ◊Ëπ Ê ∑’Ë®”‡ªìπμàÕ°“√æ—≤π“„À⇪ìπæ◊™ ¡ÿπ‰æ√
+ 5.0 + Nakornrasima
2.1
‡»√…∞°‘®μàÕ‰ª
+ PhetburiKLG
+ 24.4
√ÿª
+ Nakornnayok
+ 5.6
°“√»÷°…“≈“¬æ‘¡æ奒‡ÕÁπ‡Õ ‰¥â‡°Á∫μ—«Õ¬à“߇∂“«—≈¬å
+ KanWangyen
+-29.2
‡ª√’¬ß®“°·À≈àßμà“ß Ê 19 μ—«Õ¬à“ß (√Ÿª∑’Ë 1, 2, 3, 4 ·≈– 5)
+ Pitsanulok
+-99.9 ·≈–‰¥â»÷°…“≈—°…≥–¿“¬πÕ°æ∫«à“≈—°…≥–¿“¬πÕ°‰¡à¡’
+ 5.5 + Lopburi
§«“¡·μ°μà“ß°—π¬°‡«âπ¢π“¥‡∂“¢Õß®—ßÀ«—¥æ‘…≥ÿ‚≈° ¡’‡∂“
2.2
+ NakornpatKU „À≠à∑’Ë ÿ¥¡’¢π“¥‡ âπºà“π»Ÿπ¬å°≈“߇©≈’ˬ 6.0 ´¡. ·≈–
+-27.2
®—ßÀ«—¥≈æ∫ÿ√’ ¡’‡∂“‡≈Á°∑’Ë ÿ¥ ‚¥¬¡’¢π“¥‡ âπºà“π»Ÿπ¬å°≈“ß
| + Bureerum
‡∑à“°—∫ 1.7 ´¡. (μ“√“ß∑’Ë 1) ‡¡◊ËÕμ√«®«‘‡§√“–Àå≈“¬æ‘¡æ奒‡ÕÁπ‡Õ
+-39.5
+ Bangkok
‚¥¬«‘‡§√“–À姫“¡ —¡æ—π∏宓°¢âÕ¡Ÿ≈·∂∫¥’‡ÕÁπ‡Õ‚¥¬„™â
‚ª√·°√¡ WinBoot (√Ÿª∑’Ë 6, μ“√“ß∑’Ë 2, 3 ·≈–·ºπ¿Ÿ¡‘∑’Ë 1)
+ Pracheanburi
3
æ∫«à“·∫à߇ªìπ 3 °≈ÿà¡ (μ“√“ß∑’Ë 2 ·≈– 3, ·ºπ¿Ÿ¡‘∑’Ë 1) ¡’
·ºπ¿Ÿ¡‘∑’Ë 1 ·ºπ¿Ÿ¡‘º≈°“√«‘‡§√“–Àå≈“¬æ‘¡æ奒‡ÕÁπ‡Õ √«¡ 20
°≈ÿà¡∑’Ë 1, 2 ·≈– 3 ‚¥¬°≈ÿà¡∑’Ë 3 ®.ª√“®’π∫ÿ√’·¬°ÕÕ°‰ªμà“ß
μ—«Õ¬à“ß
À“° à«πμ—«Õ¬à“ß®“° ®.‡æ™√∫ÿ√’ (‡¢“°√–ªÿ°), ª√–®«∫§’√’¢—π∏å
(‡ “«¿“), √–·°â«, ®.π§√ª∞¡ (¡.¡À‘¥≈ «‘∑¬“‡¢μ»“≈“¬“)

°√√¡‰¥âÕ¬à“ß∂Ÿ°μâÕߥ—ß ‡™àπ °“√»÷°…“°“√„™â‡∑§π‘§ ·≈–·§≈≈— ®—¥Õ¬Ÿà„π °≈ÿ࡬àÕ¬∑’Ë 1.1 ·≈–®.ππ∑∫ÿ√’, √–¬Õß
Intersimple sequence repeat (ISSR) markers „π°“√ ·≈–®—π∑∫ÿ√’ ®—¥Õ¬Ÿà„π°≈ÿ࡬àÕ¬∑’Ë 1.2 ´÷Ëß°≈ÿ࡬àÕ¬∑—Èß Õß®—¥
μ√«® Õ∫°“√®”·π°æ—π∏ÿå∑ÿ‡√’¬π æ∫«à“«‘∏’°“√π’È “¡“√∂„™â„π Õ¬Ÿà„π°≈ÿà¡∑’Ë 1 „π¢≥–∑’Ë°≈ÿà¡∑’Ë 2 ¡’ Õß°≈ÿ࡬àÕ¬‚¥¬

°“√®”·π°æ—π∏ÿ剥âÕ¬à“ß¡’ª√– ‘∑∏‘¿“æ ‚¥¬ “¡“√∂·¬°æ—π∏ÿå ®.¢Õπ·°àπ, ®.ª√–®«∫§’√’¢—π∏å (Õà“«πâÕ¬), ®.π§√√“™ ’¡“,
∑ÿ‡√’¬πæ—π∏ÿå°“√§â“ 4 æ—π∏ÿ剥âÕ¬à“ß™—¥‡®π ‰¥â·°à æ—π∏ÿåÀ¡Õπ∑Õß ®.‡æ™√∫ÿ√’ (°≈—¥À≈«ß) ·≈–®.π§√𓬰 Õ¬Ÿà„π°≈ÿ࡬àÕ¬∑’Ë 2.1
°√–¥ÿ¡ °â“𬓫 ·≈–™–π’ Õ’°∑—È߬—ß “¡“√∂μ√«® Õ∫§«“¡ à«π ®.°“≠®π∫ÿ√’ («—߇¬Áπ), ®.æ‘…≥ÿ‚≈°, ®.≈æ∫ÿ√’, ®.π§√ª∞¡

·μ°μà“ß√–À«à“ßμâπ„πæ—π∏ÿ凥’¬«°—π (plant-to-plant variation) (¡.‡°…μ√»“ μ√å «‘∑¬“‡¢μ°”·æß· π), ®.∫ÿ√’√—¡¬å·≈–°√ÿ߇∑æœ