Page 40 - journal-9-2-Full

Journal of Thai Traditional & Alternative Medicine Vol. 9 No. 2 September-December 2011 117

º‘«∫“ߢÕ߇∂“«—≈¬å‡ª√’¬ß 20 μ—«Õ¬à“߇ª√’¬∫‡∑’¬∫°—∫‡∂“«—≈¬å

‡ª√’¬ßÕâ“ßÕ‘ß æ∫«à“ ∑ÿ°μ—«Õ¬à“߇¡◊ËÕμ√«® Õ∫¥â«¬°√¥
°”¡–∂—π„Àâ√Ÿª·∫∫¢Õß√ߧ‡≈¢º‘«∫“ß∑’˧≈⓬§≈÷ß°—π (√Ÿª∑’Ë
7)

«‘®“√≥å

°“√»÷°…“≈—°…≥–∑“ß°“¬¿“¬¿“¬πÕ°¢Õ߇∂“«—≈¬å‡ª√’¬ß

´÷Ë߇°Á∫μ—«Õ¬à“ß®“° ∂“π∑’Ëμ“¡¿“§μà“ß Ê ¡’≈—°…≥–‰¡à·μ°
μà“ß°—π¬°‡«âπ¢π“¥¢Õ߇∂“¢Õßμ—«Õ¬à“ß®“°æ‘…≥ÿ‚≈° ¡’¢π“¥

„À≠à∑’Ë ÿ¥‚¥¬¡’¢π“¥‡ âπºà“π»Ÿπ¬å°≈“߇©≈’ˬ 6 ‡´π쑇¡μ√
·≈–μ—«Õ¬à“ß®“°®—ßÀ«—¥≈æ∫ÿ√’¡’¢π“¥‡≈Á°∑’Ë ÿ¥ §◊Õ¡’‡ âπºà“π
»Ÿπ¬å°≈“߇©≈’ˬ 1.7 ‡´π쑇¡μ√ ·μà≈“¬æ‘¡æ奒‡ÕÁπ‡Õ¢Õß∑—Èß

Õßμ—«Õ¬à“ß∂Ÿ°®—¥Õ¬Ÿà„π°≈ÿ࡬àÕ¬∑’Ë 2.2 · ¥ß„Àâ‡ÀÁπ«à“¢π“¥
¢Õ߇∂“Õ“®¢÷Èπ°—∫Õ“¬ÿ¢Õßμâ𠥑πøÑ“Õ“°“» æ◊Èπ∑’˪≈Ÿ° πÈ”

·≈–¥‘π∑’˪≈Ÿ° ®“°°“√æ‘ Ÿ®πå‡Õ°≈—°…≥å∑“߇§¡’¥â«¬«‘∏’
‚§√¡“‚∑°√“øﺑ«∫“ßæ∫«à“ ∑ÿ°μ—«Õ¬à“ß¡’√Ÿª·∫∫¢Õß√ߧ‡≈¢
º‘«∫“ß∑’˧≈⓬§≈÷ß°—π ‡π◊ËÕß®“°°“√μ√«®¥â«¬°√¥°”¡–∂—π´÷Ëß

‡ªìπ Universal reagent ∑”„Àâμ√«® Õ∫ “√∑ÿ쑬¿Ÿ¡‘‰¥â
À≈“¬°≈ÿࡉ¥â¡“°°«à“ (√Ÿª∑’Ë 7) ¥—ßπ—Èπ°“√μ√«® Õ∫«‘∏’∑“ß
‡§¡’‰¡à “¡“√∂®”·π°§«“¡·μ°μà“ß„π√–¥—∫‚¡‡≈°ÿ≈¢Õß

‡∂“«—≈¬å‡ª√’¬ß ·μà “¡“√∂®”·π°‰¥â‚¥¬≈“¬æ‘¡æ奒‡ÕÁπ‡Õ
°“√μ√«®«‘‡§√“–Àå≈“¬æ‘¡æ奒‡ÕÁπ‡Õ ·∫àß°≈ÿà¡¢Õ߇∂“«—≈¬å‡ª√’¬ß
‡ªìπ 3 °≈ÿà¡„À≠à À√◊Õ 5 °≈ÿ࡬àÕ¬¥—ßμ“√“ß∑’Ë 2

‡ªìπ∑’Ëπà“ —߇°μ«à“·§≈≈— ∑’Ë™—°π”„À⇰‘¥®“° à«π¢Õß
‡¡≈Á¥‡∂“«—≈¬å‡ª√’¬ß´÷Ë߇ªìπ‡¡≈Á¥æ—π∏ÿå∑’ˉ¥â¡“®“°æ◊Èπ∑’Ë∫√‘‡«≥

¡À“«‘∑¬“≈—¬¡À‘¥≈ «‘∑¬“‡¢μ»“≈“¬“ ®—ßÀ«—¥π§√ª∞¡
√Ÿª∑’Ë 6 ·Õ°“‚√ ‡®≈Õ‘‡≈Á°‚∑√‚ø√’´‘ ¢Õß·∂∫¥’‡ÕÁπ‡Õ∑’ˉ¥â®“°
μ√«®æ∫«à“¡’√Ÿª·∫∫¢Õß·∂∫¥’‡ÕÁπ‡Õμ√ß°—∫μ—«Õ¬à“ß„∫®“°
ªØ‘°‘√‘¬“æ’´’Õ“√å °—∫‰æ√凡Õ√å M15
¡À“«‘∑¬“≈—¬¡À‘¥≈ «‘∑¬“‡¢μ»“≈“¬“ ‡™àπ°—π (√Ÿª∑’Ë 6 À¡“¬‡≈¢
À¡“¬‡Àμÿ : M = ¥’‡ÕÁπ‡Õ¢π“¥¡“μ√∞“π 100-base pairs DNA
5 ·≈– 4 μ“¡≈”¥—∫) Õ’°∑—È߬—ß∂Ÿ°®—¥„ÀâÕ¬Ÿà„π°≈ÿ࡬àÕ¬∑’Ë 1.1
marker (BioExcellece), C = ™ÿ¥§«∫§ÿ¡, 1 = √–¬Õß,
‡™àπ‡¥’¬«°—π (μ“√“ß∑’Ë 2) à«πμ—«Õ¬à“ß®“° ®—ßÀ«—¥√–¬Õß
2 = ®—π∑∫ÿ√’, 3 = ππ∑∫ÿ√’, 4 = π§√ª∞¡ (¡.¡À‘¥≈
«‘∑¬“‡¢μ»“≈“¬“), 5 = ·§≈≈— , 6 = π§√𓬰, 7 ®—π∑∫ÿ√’ ·≈–ππ∑∫ÿ√’ ∂Ÿ°®—¥„ÀâÕ¬Ÿà„π°≈ÿ࡬àÕ¬∑’Ë 1.2 ‡¥’¬«°—π
= ª√“®’π∫ÿ√’, 8 = √–·°â«, 9 = π§√ª∞¡ ¡’§à“ SI √–À«à“ß®—ßÀ«—¥√–¬Õß°—∫®—ßÀ«—¥®—π∑∫ÿ√’‡∑à“°—∫ 0.5882
(¡.‡°…μ√»“ μ√å «‘∑¬“‡¢μ°”·æß· π), 10 = ·≈–®—ßÀ«—¥√–¬Õß°—∫®—ßÀ«—¥ππ∑∫ÿ√’‡∑à“°—∫ 0.3333 (μ“√“ß∑’Ë 3)
æ‘…≥ÿ‚≈°, 11 = ≈æ∫ÿ√’, 12 = ∫ÿ√’√—¡¬å, 13 =
‡π◊ËÕß®“°‡∂“∑’ˇ°Á∫®“°μâπ∑’Ë ®—ßÀ«—¥ππ∑∫ÿ√’ ·≈–®—ßÀ«—¥√–¬Õß
°√ÿ߇∑æœ, 14 = ‡æ™√∫ÿ√’, 15 = ª√–®«∫§’√’¢—π∏å
‡ªìπμâπ∑’Ëπ”¡“®“°μâπ·¡àæ—π∏ÿ宓° «π ¡ÿπ‰æ√ °√¡
( ∂“π‡ “«¿“), 16 = ¢Õπ·°àπ, 17 = π§√√“™ ’¡“,
«‘∑¬“»“ μ√å°“√·æ∑¬å ®—ßÀ«—¥®—π∑∫ÿ√’ · ¥ß„Àâ‡ÀÁπ«à“°“√„™â
18 = °“≠®π∫ÿ√’, 19 = ‡æ™√∫ÿ√’ ·≈– 20 =
ª√–®«∫§’√’¢—π∏å (Õà“«πâÕ¬) ≈“¬æ‘¡æ奒‡ÕÁπ‡Õ “¡“√∂·¬°§«“¡·μ°μà“ß∑“ߥâ“πæ—π∏ÿ