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«“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° Vol. 5 No. 1 January-April 2007
Original Article

An Antioxidative and Cytotoxic Substance

Extracted from Curcuma comosa Roxb.

Somchit Niumsakul*
Angkana Hirunsaree*
Suvara Wattanapitayakul †
Nuchatra Junsuwanitch*
Kasem Prapanupun*

Abstract
The powerful antioxidative and HeLa cytotoxic activity of crude ethanol extract of Curcuma comosa
Roxb. prompted us to carry out biologically-guided separation of the active constituent. Chromatographic
separation of the ethanol extract yielded fractions Zcc(R)-E-1 and Zcc(R)-E-2. The potent fraction Zcc(R)-E-
1 was subsequently bioassay-guided separated and the active fraction Zcc(R)-E-15 was obtained. Recrystal-
lization of fraction Zcc(R)-E-15 yielded compound A, exhibited antioxidant power 1064.73 ± 60.96 μM vita-
min C equivalence and HeLa cell cytotoxicity with IC50 4.44 ± 0.85 μg/ml. With structure elucidation via
spectroscopic techniques, compound A was identified as 4,6-dihydroxy-2-O-(β-D-glucopyranosyl)acetophenone.
Key words: Curcuma comosa, antioxidant, 4,6-dihydroxy-2-O-(β-D-glucopyranosyl)acetophenone

Introduction compounds, some of which possess interesting bio-

Several plants belonging to the genus Curcuma logical activities. C. comosa was originally cultivated
of the family Zingiberaceae, i.e., Curcuma aromatica in Indonesia, where it is known as Temoelawak, and
Salisb., C. comosa Roxb., C. longa Linn., C. parviflora spread to several areas in the Indo-Malaysian terri-
Wall., C. xanthorrhiza Roxb. and C. zedoaria Roxb., tory as well as Thailand. In Thailand, this plant is
1
have been used as traditional medicine for the treat- commonly known as Waan chak mod luuk , and has
ment of a wide range of diseases in many Asian coun- been used widely by traditional practitioners for the
tries including Thailand. Phytochemical investiga- treatment of postpartum uterine inflammation and also
2
tions of these plants led to the isolation of several as an aromatic stomachic and cholerctic . In 1994
Jurgen et al. reported the nematicidal activity of
3
diarylheptanoids, and the active principal agents in
*Medicinal Plant Research Institute, Department of Medical Sciences, hexane-soluble fraction of the methanol extract of C.
Ministry of Public Health, Nonthaburi 11000
4-8
† Department of Pharmacology, Faculty of Medicine, Srinakharinwirot comosa. Piyachaturawat et al. investigated the bio-
University, Prasanmitr, Bangkok 10110 logical activities of C. comosa crude extracts. It was

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