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keto group (C=O). All these described spectroscopic Piyachaturawat, Department of Physiology, Faculty of
data implied that compound A was 4,6-dihydroxy-2- Science, Mahidol University, for their valuable sugges-
O-(β-D-glucopyranosyl)acetophenone. tions in this study.
The phloracetophenone glucoside fraction Zcc(R)-
E15, showed total antioxidant capacity of 1064.73 ± References
60.96 μM equivalent to vitamin C. Furthermore, it
was found to be cytotoxic to HeLa cells and had an 1. Smitinand T. Thai plant names. (Revised edition). Bangkok : Prachachon
Co., Ltd.; 2001.
IC value of 4.44 ± 0.85 μg/ml. These results showed 2. Piyachaturawat P, Chai-ngam N, Chuncharunee A, Komaratat P,
50
clearly that the isolated phloracetophenone glucoside Suksamrarn A. Choleretic activity of phloracetophenone in rats:
fraction was relatively less potent than the ethanol structure-function studies using acetophenone analogues. European
extract, Zcc(R) as shown in Tables 1 and 2. There- J Pharmacol 2000;387:221-7.
fore, C. comosa may contain more than one active 3. Jurgens TM, Frazier EG, Schaeffer JM, Jones TE, Zink DL, Borris RP,
et al. Novel nematocidal agents from Curcuma comosa. J Nat Prod
antioxidative/cytotoxic constituent and further stud- 1994;57:230-5.
ies to isolate other active constituents are recom- 4. Piyachaturawat P, Ercharuporn S, Suksamrarn A. Uterotropic effect of
mended. Curcuma comosa in rats. Int J Pharmacog 1995:33(4):334-8.
5. Piyachaturawat P, Teeratagolpisal N, Toskulkao C, Suksamrarn A.
Conclusions Hypolipidemic effect of Curcuma comosa in mice. Artery 1997;22(5):
233-41.
Ethanol extracts of Curcuma comosa rhizomes 6. Suksamrarn A, Eiamong S, Piyachaturawat P, Byrnre TL. A
were evaluated in the cytotoxicity assay in HeLa cells phloracetophenone glucoside with choleretic activity from Curcuma
and antioxidant activity by ferric reducing/antioxi- comosa. Phytochemistry 1997;45:103-5.
dant power assay (FRAP). The isolation and identifi- 7. Piyachaturawat P, Charoenpiboonsin J, Toskulkao C, Suksamrarn A.
Reduction of plasma cholesterol by Curcuma comosa extract in
cation of one of the responsible active compounds hypercholesterolaemic hamster. J Ethnopharmacol 1997;66:199-204.
was found to be 4,6-dihydroxy-2-O-(β-D-glucopy- 8. Piyachaturawat P, Timinkul A, Suksamrarn A. Effect of Curcuma comosa
ranosyl) acetophenone. extract on male fertility in rats. Pharmaceutic Biol 1999;37:22-27.
9. Schumann K. Zingiberaceae. In: Engler A, editor. Berlin : Pflanzenreich;
Acknowledgments 1903: IV. P. 46.
10. Backer CA, Bakhuizen van den Brink RC. Flora of Java. The Netherlands,
The authors would like to express deep gratitude I.N.V.P.: Noordhoff-Groningen; 1963.
to Professor Dr. Puangpen Siriruksa, Faculty of Science, 11. Styhl E. Thin-layer chromatography. In: A laboratory handbook. Berlin,
Prince of Songkhla University, and Associate Professor Heidelburg, New York: Springer-Verlag; 1969.
Dr. Pranom Chantaranothai, Faculty of Science, Khon 12. Benzie I, Strain J. Ferric reducing /antioxidant power assay. Direct
measure of total antioxidant activity of biological fluids and modified
Kaen University, for their valuable suggestions on iden- version for simultaneous measurement of total antioxidant power
tifying the plant for assay. The authors also would like and ascorbic acid concentration. Methods Enzymol 1999;299:15-27.
to thank Associate Professor Dr. Apichart Suksamrarn, 13. Takahashi GW, Montgomery RB, Stahl WL, Crittenden CA, Valentine
Department of Chemistry, Faculty of Science, MA, Thorning DR, et al. Pentoxifylline inhibits tumor necrosis factor-
alpha-mediated cytotoxicity and cytostasis in L929 murine fibrosarcoma
Ramkhamhaeng University, and Professor Dr. Pawinee cells. Int J Immunopharmacol 1994;16:723-36.