Page 35 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 5 ฉบับที่ 1

Journal of Thai Traditional & Alternative Medicine Vol. 5 No. 1 January - April 2007 ÛÛ

pole mass selective detector, HP-INNOWAX column (30 Results and Discussion
m × 0.25 mm, coated film (0.25 m in thickness). The
column was temperature programmed at 40-220 ˚C with Comparison of physical appearance of M. alba
4˚C per minute increasing rate, pulse split injector 20:1, flower oil extracted by steam distillation, hexane
at 230˚C. The detector was El 70 eV MSD and the extraction and enfleurage method
carrier gas was helium (10 psi) with a flow rate of 1.2 The physical appearance of the M. alba flower oil
ml/min and 40-450 amu in the mass scan range. The samples were carefully observed as shown in Table 1
constituents of the oil samples were identified by and Figure 2.
matching their spectra with those recorded in the MS
library (Wiley 7n and Robert P. Adams).

Table 1 Physical appearance of M. alba oil

Extraction method Physical appearance of the oil
Steam distillation Clear colorless solution; immiscible with
water; odor similar to boiled M. alba flowers
Hexane extraction Deep orange solution; immiscible with wa-
ter; odor similar to fresh M. alba flowers but
more pungent Figure 2 Oil samples from three different methods
Enfleurage method using Light yellowish solution; immiscible with 1 = oil sample from enfleurage method using buffalo fat
buffalo fat water; odor similar to fresh M. alba flowers 2 = oil sample from steam distillation
3 = oil sample from hexane extraction

Abundance

Time

Figure 3 Total ion chromatogram of oil extracted from fresh M. alba flowers by the enfleurage method.