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Journal of Thai Traditional & Alternative Medicine                        Vol. 5 No. 1 January - April 2007 ÛÛ


             pole mass selective detector, HP-INNOWAX column (30           Results and Discussion
             m × 0.25 mm, coated film (0.25 m in thickness). The
             column was temperature programmed at 40-220 ˚C with  Comparison of physical appearance of M. alba
             4˚C per minute increasing rate, pulse split injector 20:1,  flower oil extracted by steam distillation, hexane
             at 230˚C.  The detector was El 70 eV MSD and the   extraction and enfleurage method
             carrier gas was helium (10 psi) with a flow rate of 1.2  The physical appearance of the M. alba flower oil
             ml/min and 40-450 amu in the mass scan range.  The  samples were carefully observed as shown in Table 1
             constituents of the oil samples were identified by  and Figure 2.
             matching their spectra with those recorded in the MS
             library (Wiley 7n and Robert P. Adams).











             Table 1 Physical appearance of M. alba oil

              Extraction method  Physical appearance of the oil
             Steam distillation  Clear colorless solution; immiscible with
                               water; odor similar to boiled M. alba flowers
             Hexane extraction  Deep orange solution; immiscible with wa-
                               ter; odor similar to fresh M. alba flowers but
                               more  pungent                    Figure 2 Oil samples from three different methods
             Enfleurage method using Light yellowish solution; immiscible with  1 = oil sample from enfleurage method using buffalo fat
              buffalo fat      water; odor similar to fresh M. alba flowers  2 = oil sample from steam distillation
                                                                       3 = oil sample from hexane extraction




                Abundance



























                 Time

                         Figure 3  Total ion chromatogram of oil extracted from fresh M. alba flowers by the enfleurage method.
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