Page 37 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 5 ฉบับที่ 1

Journal of Thai Traditional & Alternative Medicine Vol. 5 No. 1 January - April 2007 Ûı

pounds: methyl-2-methylbutyrate (7.77%), ethyl-2-methyl derivatives were found in all oil samples with the con-
butyrate (6.76%), cis-ocimene (1.81), trans-β-ocimene tents range from below 0.5 to 66 per cent. The obvious
(2.26%), trans-linalool oxide (<0.5%), cis-linalool oxide difference was indole, which was found to be the major
(0.28%), linalool (66.92%), β-elemene (1.34%), trans- constituent in the oil sample from the enfleurage method.
caryophyllene (0.72%) and eugenol (4.52%). A blank sample of buffalo fat extract was also analyzed
by GC-MS and the chromatogram showed no peak of
Chemical composition of M. alba flower oil indole (Figure 6). Therefore, the high content of indole
extracted by hexane in the oil sample from the enfleurage method was con-
According to the total ion chromatogram in Figure firmed to be from the fresh M. alba flowers themselves.
5, M. alba flower oil consisted of the following componds: According to previous research data of the Thai-
7
cis ocimene (<0.5%), trans-β-ocimene (<0.5%), trans-lina- land Institute of Scientific and Technological Research ,
lool oxide (1.14%), cis-linalool oxide (1.43%), linalool linalool is the major component of M. alba flower oil
7
(28.92%), methyl benzoate (0.40%), 2-methylbutanoic acid extracted by steam distillation (66.94% in content) which
(33.01%), epoxylinalool (0.53%), phenyl ethylalcohol was similar to the finding of our current study which
(4.52%) phenetyl-2-methyl benzoate (5.06%) and methyl also found linalool to be the major compound with the
eugenol (3.07%). content being 66.92 per cent. Whereas other minor
compounds were methyl-2-methylbutyrate (7.77%) and
Comparison of chemical constituents in M. alba ethyl-2-methyl butyrate (6.76%), the rest of the compo-
flower oil extracted by the enfleurage method, nents were found to have a content of less than 5 per
steam distillation and hexane extraction cent for each component. In another study of Shang
From the comparison study using GC-MS chroma- and colleagues , solid phase microextraction was used
10
tography, the constituents from the three methods were to analyze the volatile constituents of M. alba flowers
generally similar in chemical classes (esters, alcohol and and the major components were α-myrcene, (S)-li-
terpene derivatives), but the yields varied. Linalool and monene, (R)-fenchone, linalool, camphor, caryophyllene,

Abundance

Absence of
indole peak

Time

Figure 6 Total ion chromatogram of compounds in blank buffalo fat