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Journal of Thai Traditional & Alternative Medicine                        Vol. 5 No. 1 January - April 2007 Ûı


             pounds: methyl-2-methylbutyrate (7.77%), ethyl-2-methyl  derivatives were found in all oil samples with the con-
             butyrate (6.76%), cis-ocimene (1.81), trans-β-ocimene  tents range from below 0.5 to 66 per cent.  The obvious
             (2.26%), trans-linalool oxide (<0.5%), cis-linalool oxide  difference was indole, which was found to be the major
             (0.28%), linalool (66.92%),  β-elemene (1.34%), trans-  constituent in the oil sample from the enfleurage method.
             caryophyllene (0.72%) and eugenol (4.52%).         A blank sample of buffalo fat extract was also analyzed
                                                                by GC-MS and the chromatogram showed no peak of
             Chemical composition of M. alba flower oil         indole (Figure 6).  Therefore, the high content of indole
             extracted by hexane                                in the oil sample from the enfleurage method was con-
                 According to the total ion chromatogram in Figure  firmed to be from the fresh M. alba flowers themselves.
             5, M. alba flower oil consisted of the following componds:  According to previous research data of the Thai-
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             cis ocimene (<0.5%), trans-β-ocimene (<0.5%), trans-lina-  land Institute of Scientific and Technological Research ,
             lool oxide (1.14%), cis-linalool oxide (1.43%), linalool  linalool is the major component of M. alba flower oil
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             (28.92%), methyl benzoate (0.40%), 2-methylbutanoic acid  extracted by steam distillation (66.94% in content)  which
             (33.01%), epoxylinalool (0.53%), phenyl ethylalcohol  was similar to the finding of our current study which
             (4.52%) phenetyl-2-methyl benzoate (5.06%) and methyl  also found linalool to be the major compound with the
             eugenol (3.07%).                                   content being 66.92 per cent.  Whereas other minor
                                                                compounds were methyl-2-methylbutyrate (7.77%) and
             Comparison of chemical constituents in M. alba     ethyl-2-methyl butyrate (6.76%), the rest of the compo-
             flower oil extracted by the enfleurage method,     nents were found to have a content of less than 5 per
             steam distillation and hexane extraction           cent for each component. In another study of Shang
                 From the comparison study using GC-MS chroma-  and colleagues , solid phase microextraction was used
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             tography, the constituents from the three methods were  to analyze the volatile constituents of M. alba flowers
             generally similar in chemical classes (esters, alcohol and  and the major components were  α-myrcene, (S)-li-
             terpene derivatives), but the yields varied.  Linalool and  monene, (R)-fenchone, linalool, camphor, caryophyllene,





               Abundance




















                                                                                 Absence of
                                                                                 indole peak






                Time

                                        Figure 6 Total ion chromatogram of compounds in blank buffalo fat
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