Page 38 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 5 ฉบับที่ 1

Ûˆ «“√ “√°“√·æ∑¬å·ºπ‰∑¬·≈–°“√·æ∑¬å∑“߇≈◊Õ° ªï∑’Ë ı©∫—∫∑’Ë Ò ¡°√“§¡ - ‡¡…“¬π Úıı

germacrene D and others. Some compounds from that Indole, the exceptional component in M. alba flower
study (such as α-myrcene, (S)-limonene, (R)-fenchone) oil from the enfleurage method, has a unique property
were not found in this current study due to the differ- as a fixation compound. It was one of the common
ence in type of GC-MS column and the variation in ingredients in various perfume formulas used to prolong
method conditions. the effect of the more volatile ingredients in the formula
Linalool, a compound commonly found as a major in an attempt to equalize the rate of evaporation of the
12
component of M. alba flowers, is a terpene alcohol found component ingredients . The content of indole in the
in many flowers and spice plants with numerous com- oil sample from the enfleurage method was relatively
mercial applications. Linalool has a pleasant floral scent large, which may be related to its unique odor being
with a touch of spiciness. However, in high concentra- different from that of the oil extracted by steam distilla-
tion, the odor of linalool may be too strong and should tion or hexane.
be avoided by people with perfume allergy . Although According to the magnified total ion chromato-
11
linalool has a pleasant scent in general, its content should grams, indole was actually found in a minuscule amount
be at an optimum concentration in a perfume. Interest- (less than 0.25%) in M. alba flower oil extracted by steam
ingly, linalool and its derivatives (cis-linalool oxide, trans- distillation (Figure 7) but totally absent in that produced
linalool oxide) were found in all the oil samples from the by hexane extraction (Figure 8). The indole peak disap-
three selected methods, which indicated that an oxida- peared from the original GC-MS chromatogram of the
tion reaction occurred during the extraction processes. oil sample from steam distillation because its abundance
Linalool derivatives were found in minor amounts (all was below the detection limit.
less than 1.5%), whereas parent linalool was found in When considering the extraction processes, the
much higher amounts (28.92- 66.92%). enfleurage method is a gentle technique. Fresh M. alba

Abundance

indole

Time

Figure 7 Magnified total ion chromatogram of oil extracted from fresh M. alba flowers by steam distillation (with the indole peak being in
minor abundance).