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              Õ∫¥â«¬μŸâÕ∫≈¡√âÕπ (hot air oven) ∑’ËÕÿ≥À¿Ÿ¡‘ 40   √â“ß°√“ø¡“μ√∞“π (standard curve) ‚¥¬

                                                                     2
              Õß»“‡´≈‡´’¬  ‡ªìπ√–¬–‡«≈“ 72 ™—Ë«‚¡ß 𔉪∫¥  °”Àπ¥„Àâ§à“ r  = 0.995 ± 0.05
              À¬“∫ ·≈â«∫√√®ÿºß¥Õ°Õ—≠™—π≈ß„π´Õ߇¬◊ËÕ°√–¥“…      “√≈–≈“¬μ—«Õ¬à“ß∑”‡™àπ‡¥’¬«°—∫ “√≈–≈“¬

              ´Õß≈– 2 °√—¡ ªî¥ºπ÷°´Õߥ⫬‡§√◊ËÕß´’≈§«“¡√âÕπ  ¡“μ√∞“π°√¥·°≈≈‘° ‡¡◊ËÕ‰¥â§à“°“√¥Ÿ¥°≈◊π· ß
              ·≈–‡°Á∫√—°…“„π¿“™π–ªî¥                      ¢Õß “√≈–≈“¬μ—«Õ¬à“ß·≈â« π”‰ª§”π«≥À“ª√‘¡“≥
                   3. Õÿ≥À¿Ÿ¡‘·≈–‡«≈“∑’ˇÀ¡“– ¡„π°“√‡μ√’¬¡   “√ª√–°Õ∫øïπÕ≈‘°√«¡‚¥¬‡∑’¬∫°—∫°√“ø¡“μ√∞“π

              πÈ”¥Õ°Õ—≠™—π                                ·≈–√“¬ß“πº≈‡ªìπ mg gallic acid/g
                   ‡μ√’¬¡™“¥Õ°Õ—≠™—π‚¥¬°“√π”´Õß™“¢π“¥ 2       5. °“√»÷°…“ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–„π™“¥Õ°

              °√—¡ ¡“·™à„ππÈ”√âÕπ 200 ¡‘≈≈‘≈‘μ√ ∑’Ë¡’Õÿ≥À¿Ÿ¡‘  Õ—≠™—π
              ·μ°μà“ß°—π 3 √–¥—∫ §◊Õ 60, 80 ·≈– 100 Õß»“      »÷°…“ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–¥â«¬«‘∏’ DPPH (1,1-
              ‡´≈‡´’¬  ‚¥¬·™à‡ªìπ√–¬–‡«≈“ 3 √–¥—∫ ‰¥â·°à 5, 10  diphenyl-2-picrylhydrazyl) radical scavenging

              ·≈– 15 π“∑’ ·≈â«π”∂ÿß™“ÕÕ° ∑”„À⇬Áπ∑’ËÕÿ≥À¿Ÿ¡‘  activity assay ´÷Ë߇ªìπ«‘∏’∑’Ë¥—¥·ª≈ß¡“®“° Bondet
                                                                     [12]
              ÀâÕß ®“°π—Èππ”πÈ”™“¡“°√Õߥ⫬°√–¥“…°√Õß     et al. (1997)  ‚¥¬„™â «‘μ“¡‘π´’ (L-Ascorbic
              Whatman ‡∫Õ√å 1 ‡°Á∫√—°…“„π¢«¥·°â« ’™“°àÕπ  acid) ‡ªìπ “√≈–≈“¬¡“μ√∞“π ‡μ√’¬¡ “√≈–≈“¬

              𔉪«‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡    μ—«Õ¬à“ß∑’˧«“¡‡¢â¡¢âπμà“ß Ê ·≈â«‡μ‘¡ “√≈–≈“¬
              ·≈–»÷°…“ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–                DPPH (0.2 ¡‘≈≈‘‚¡≈“√å) 4,500 ‰¡‚§√≈‘μ√ ‡¢¬à“„Àâ
                   4. °“√«‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°  ‡¢â“°—π μ—Èß∑‘È߉«â„π∑’Ë¡◊¥π“π 30 π“∑’ ®“°π—Èπ𔉪

              √«¡„π™“¥Õ°Õ—≠™—π                            «—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’˧«“¡¬“«§≈◊Ëπ 517 π“‚π‡¡μ√
                   «‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡   ¥â«¬‡§√◊ËÕß ‡ª°‚μ√‚ø‚μ¡‘‡μÕ√å (UV-spectropho-

              ¥â«¬«‘∏’ Folin-Ciocalteu ´÷Ë߇ªìπ«‘∏’∑’Ë¥—¥·ª≈ß¡“®“°  tometer) ·≈â«π”§à“°“√¥Ÿ¥°≈◊π· ß∑’ˉ¥â (A) ¡“
                               [11]
              Vernon et al. (1999)  ‚¥¬„™â°√¥·°≈≈‘° (gal-  §”π«≥‡ªÕ√凴Áπμå°“√¬—∫¬—ÈßÕπÿ¡Ÿ≈Õ‘ √– DPPH (%
              lic acid) ‡ªìπ “√≈–≈“¬¡“μ√∞“π               DPPH radical scavenging activity) μ“¡

                   ‡μ√’¬¡ “√≈–≈“¬¡“μ√∞“π°√¥·°≈≈‘° ∑’Ë      ¡°“√¥—ßμàÕ‰ªπ’È 1 DPPH radical scavenging
              §«“¡‡¢â¡¢âπ‡∑à“°—∫ 0.1, 0.2, 0.3, 0.4 ·≈– 0.5  activity (%) = [(Acontrol-Asample)/Acontrol]

              ¡‘≈≈‘°√—¡μàÕ¡‘≈≈‘≈‘μ√ ·≈â«‡μ‘¡ Folin-Ciocalteu  x 100 (‚¥¬∑’Ë Acontrol ·≈– Asample §◊Õ
              reagent 400 ‰¡‚§√≈‘μ√  “√≈–≈“¬‚´‡¥’¬¡        “√≈–≈“¬ DPPH ∑’ˉ¡à‡μ‘¡ “√μ—«Õ¬à“ß ·≈–
              §“√å∫Õ‡πμ (7.5%) 1,000 ‰¡‚§√≈‘μ√ ·≈–πÈ”°≈—Ëπ   “√≈–≈“¬ DPPH ∑’Ë‡μ‘¡ “√μ—«Õ¬à“ß μ“¡≈”¥—∫)

              2,000 ‰¡‚§√≈‘μ√ μ“¡≈”¥—∫ ‡¢¬à“„À⇢⓰—π μ—Èß∑‘Èß
                                                          °“√«‘‡§√“–Àå¢âÕ¡Ÿ≈∑“ß ∂‘μ‘
              ‰«â„π∑’Ë¡◊¥π“π 30 π“∑’ ·≈â«®÷ß«—¥§à“¥Ÿ¥°≈◊π· ß∑’Ë
              §«“¡¬“«§≈◊Ëπ 756 π“‚π‡¡μ√ ¥â«¬‡§√◊ËÕß ‡ª°‚μ√-   «‘‡§√“–Àå¢âÕ¡Ÿ≈∑—Ë«‰ª‚¥¬„™â ∂‘쑇™‘ßæ√√≥π“

              ‚ø‚μ¡‘‡μÕ√å (UV-spectrophotometer) π”§à“    ‰¥â·°à §à“‡©≈’ˬ ·≈–§à“‡∫’ˬ߇∫π¡“μ√∞“π ·≈–„™â
              °“√¥Ÿ¥°≈◊π· ß∑’ˉ¥â (A) ·≈–§«“¡‡¢â¡¢âπ (C) ‰ª   ∂‘쑇™‘ßÕπÿ¡“π ‰¥â·°à  ∂‘μ‘ One Way ANOVA
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