Page 42 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 16 ฉบับที่ 3
P. 42
376
„π°“√‡ª√’¬∫‡∑’¬∫ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡
% DPPH radical scavenging activity
·≈–ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–„π™“¥Õ°Õ—≠™—π∑’Ë„™â
Õÿ≥À¿Ÿ¡‘·≈–√–¬–‡«≈“„π°“√™ß™“·μ°μà“ß°—π 3
√–¥—∫
º≈°“√»÷°…“
®“°°“√«‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°
√«¡·≈–»÷°…“ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–„π™“¥Õ°Õ—≠™—π
§«“¡‡¢â¡¢âπ„π “√≈–≈“¬ Ascobic acid (mg/ml)
¥â«¬«‘∏’ Folin-Ciocalteu Colorimetric ·≈– DPPH
√Ÿª∑’Ë 2 °√“ø¢Õß “√≈–≈“¬¡“μ√∞“π Ascorbic acid
radical scavenging activity assay μ“¡≈”¥—∫ (∑’˧«“¡‡¢â¡¢âπ 0.01-0.15 mg/ml)
‰¥â°√“ø¢Õß “√≈–≈“¬¡“μ√∞“π Gallic acid ·≈–
L-Ascobic Acid ¥—ß· ¥ß„π√Ÿª∑’Ë 1 ·≈– 2 ´÷Ëß¡’ ¥Õ°Õ—≠™—π°—∫ L-Ascobic Acid æ∫«à“ ™“¥Õ°
¡°“√‡ âπμ√ß §◊Õ y = 1.8618x - 0.0137 ·≈– y = Õ—≠™—π¡’ª√– ‘∑∏‘¿“æ„π°“√μâ“πÕπÿ¡Ÿ≈Õ‘ √–πâÕ¬°«à“
424.87x + 17.891 μ“¡≈”¥—∫ L-Ascobic Acid ‚¥¬§«“¡‡¢â¡¢âπ∑’Ë„™â„π°“√
º≈°“√∑¥≈Õß æ∫«à“ ¿“«–∑’ˇÀ¡“– ¡„π°“√ °”®—¥Õπÿ¡Ÿ≈Õ‘ √–‰¥â 50% ¢Õß L-Ascobic Acid
™ß™“¥Õ°Õ—≠™—π §◊Õ °“√™ß∑’ËÕÿ≥À¿Ÿ¡‘ 80 Õß»“ ‡∑à“°—∫ 0.0756 mg/ml
‡´≈‡´’¬ ‡ªìπ√–¬–‡«≈“π“π 10 π“∑’ ‡π◊ËÕß®“°¡’ ®“°°“√‡μ√’¬¡™“¥Õ°Õ—≠™—π∑’ËÕÿ≥À¿Ÿ¡‘·≈–
ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡·≈–ƒ∑∏‘Ï„π°“√ √–¬–‡«≈“μà“ß Ê ·≈â«π”¡“«‘‡§√“–ÀåÀ“ª√‘¡“≥
μâ“πÕπÿ¡Ÿ≈Õ‘ √–¥’∑’Ë ÿ¥ ´÷Ëß¡’§à“‡∑à“°—∫ 47.56 ± 1.36 “√ª√–°Õ∫øïπÕ≈‘°√«¡ æ∫«à“ Õÿ≥À¿Ÿ¡‘„π°“√™ß
mg GAE/g ·≈– 31.19 ± 1.27 % μ“¡≈”¥—∫ ¥—ß ™“¥Õ°Õ—≠™—π∑’Ë√–¬–‡«≈“ 10 π“∑’ (60, 80 ·≈– 100
· ¥ß„πμ“√“ß∑’Ë 1 ·≈–‡¡◊ËÕ∑”°“√‡ª√’¬∫‡∑’¬∫™“ Õß»“‡´≈‡´’¬ ) ¡’º≈μàÕª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°
√«¡·μ°μà“ß°—πÕ¬à“ß¡’π—¬ ”§—≠∑“ß ∂‘μ‘ (p < 0.05)
§à“¥Ÿ¥°≈◊π· ß∑’˧«“¡¬“«§≈◊Ëπ 765 nm ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡ Ÿß ÿ¥ ‡∑à“°—∫ 47.56
‚¥¬∑’ËÕÿ≥À¿Ÿ¡‘ 80 Õß»“‡´≈‡´’¬ ™“¥Õ°Õ—≠™—π¡’
± 1.36 mg GAE/g √Õß¡“§◊Õ ∑’ËÕÿ≥À¿Ÿ¡‘ 100 ·≈–
60 Õß»“‡´≈‡´’¬ ‡∑à“°—∫ 33.16 ± 0.54 ·≈– 32.10
± 1.03 mg GAE/g μ“¡≈”¥—∫ πÕ°®“°π—Èπæ∫«à“
√–¬–‡«≈“„π°“√™ß™“¥Õ°Õ—≠™—π∑’ËÕÿ≥À¿Ÿ¡‘ 80
Õß»“‡´≈‡´’¬ (5, 10 ·≈– 15 π“∑’) ¬—ß¡’º≈μàÕ
ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡Õ’°¥â«¬ ‚¥¬∑’Ë
§«“¡‡¢â¡¢âπ “√≈–≈“¬¡“μ√∞“π Gallic acid (mg/ml)
√–¬–‡«≈“ 10 π“∑’ ™“¥Õ°Õ—≠™—π¡’ª√‘¡“≥ “√
√Ÿª∑’Ë 1 °√“ø¢Õß “√≈–≈“¬¡“μ√∞“π Gallic acid (∑’Ë
§«“¡‡¢â¡¢âπ 0.1-0.5 mg/ml) ª√–°Õ∫øïπÕ≈‘°√«¡ Ÿß ÿ¥ ‡∑à“°—∫ 47.56 ± 1.36 mg
