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             „π°“√‡ª√’¬∫‡∑’¬∫ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡
                                                          % DPPH radical scavenging  activity
             ·≈–ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–„π™“¥Õ°Õ—≠™—π∑’Ë„™â
             Õÿ≥À¿Ÿ¡‘·≈–√–¬–‡«≈“„π°“√™ß™“·μ°μà“ß°—π 3

             √–¥—∫

                           º≈°“√»÷°…“


                 ®“°°“√«‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°
             √«¡·≈–»÷°…“ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–„π™“¥Õ°Õ—≠™—π
                                                                  §«“¡‡¢â¡¢âπ„π “√≈–≈“¬ Ascobic acid (mg/ml)
             ¥â«¬«‘∏’ Folin-Ciocalteu Colorimetric ·≈– DPPH
                                                         √Ÿª∑’Ë 2 °√“ø¢Õß “√≈–≈“¬¡“μ√∞“π Ascorbic acid
             radical scavenging activity assay μ“¡≈”¥—∫        (∑’˧«“¡‡¢â¡¢âπ 0.01-0.15 mg/ml)
             ‰¥â°√“ø¢Õß “√≈–≈“¬¡“μ√∞“π Gallic acid ·≈–

             L-Ascobic Acid ¥—ß· ¥ß„π√Ÿª∑’Ë 1 ·≈– 2 ´÷Ëß¡’  ¥Õ°Õ—≠™—π°—∫ L-Ascobic Acid æ∫«à“ ™“¥Õ°
              ¡°“√‡ âπμ√ß §◊Õ y = 1.8618x - 0.0137 ·≈– y =  Õ—≠™—π¡’ª√– ‘∑∏‘¿“æ„π°“√μâ“πÕπÿ¡Ÿ≈Õ‘ √–πâÕ¬°«à“
             424.87x + 17.891 μ“¡≈”¥—∫                   L-Ascobic Acid ‚¥¬§«“¡‡¢â¡¢âπ∑’Ë„™â„π°“√

                 º≈°“√∑¥≈Õß æ∫«à“  ¿“«–∑’ˇÀ¡“– ¡„π°“√   °”®—¥Õπÿ¡Ÿ≈Õ‘ √–‰¥â 50% ¢Õß L-Ascobic Acid
             ™ß™“¥Õ°Õ—≠™—π §◊Õ °“√™ß∑’ËÕÿ≥À¿Ÿ¡‘ 80 Õß»“  ‡∑à“°—∫ 0.0756 mg/ml
             ‡´≈‡´’¬  ‡ªìπ√–¬–‡«≈“π“π 10 π“∑’ ‡π◊ËÕß®“°¡’    ®“°°“√‡μ√’¬¡™“¥Õ°Õ—≠™—π∑’ËÕÿ≥À¿Ÿ¡‘·≈–

             ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡·≈–ƒ∑∏‘Ï„π°“√      √–¬–‡«≈“μà“ß Ê ·≈â«π”¡“«‘‡§√“–ÀåÀ“ª√‘¡“≥
             μâ“πÕπÿ¡Ÿ≈Õ‘ √–¥’∑’Ë ÿ¥ ´÷Ëß¡’§à“‡∑à“°—∫ 47.56 ± 1.36   “√ª√–°Õ∫øïπÕ≈‘°√«¡ æ∫«à“ Õÿ≥À¿Ÿ¡‘„π°“√™ß

             mg GAE/g ·≈– 31.19 ± 1.27 % μ“¡≈”¥—∫ ¥—ß    ™“¥Õ°Õ—≠™—π∑’Ë√–¬–‡«≈“ 10 π“∑’ (60, 80 ·≈– 100
             · ¥ß„πμ“√“ß∑’Ë 1 ·≈–‡¡◊ËÕ∑”°“√‡ª√’¬∫‡∑’¬∫™“  Õß»“‡´≈‡´’¬ ) ¡’º≈μàÕª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°
                                                         √«¡·μ°μà“ß°—πÕ¬à“ß¡’π—¬ ”§—≠∑“ß ∂‘μ‘ (p < 0.05)
               §à“¥Ÿ¥°≈◊π· ß∑’˧«“¡¬“«§≈◊Ëπ 765 nm       ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡ Ÿß ÿ¥ ‡∑à“°—∫ 47.56
                                                         ‚¥¬∑’ËÕÿ≥À¿Ÿ¡‘ 80 Õß»“‡´≈‡´’¬  ™“¥Õ°Õ—≠™—π¡’



                                                         ± 1.36 mg GAE/g √Õß¡“§◊Õ ∑’ËÕÿ≥À¿Ÿ¡‘ 100 ·≈–
                                                         60 Õß»“‡´≈‡´’¬  ‡∑à“°—∫ 33.16 ± 0.54 ·≈– 32.10
                                                         ± 1.03 mg GAE/g μ“¡≈”¥—∫ πÕ°®“°π—Èπæ∫«à“

                                                         √–¬–‡«≈“„π°“√™ß™“¥Õ°Õ—≠™—π∑’ËÕÿ≥À¿Ÿ¡‘ 80
                                                         Õß»“‡´≈‡´’¬  (5, 10 ·≈– 15 π“∑’) ¬—ß¡’º≈μàÕ
                                                         ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°√«¡Õ’°¥â«¬ ‚¥¬∑’Ë
                   §«“¡‡¢â¡¢âπ “√≈–≈“¬¡“μ√∞“π Gallic acid (mg/ml)
                                                         √–¬–‡«≈“ 10 π“∑’ ™“¥Õ°Õ—≠™—π¡’ª√‘¡“≥ “√
             √Ÿª∑’Ë 1 °√“ø¢Õß “√≈–≈“¬¡“μ√∞“π Gallic acid (∑’Ë
                   §«“¡‡¢â¡¢âπ 0.1-0.5 mg/ml)            ª√–°Õ∫øïπÕ≈‘°√«¡ Ÿß ÿ¥ ‡∑à“°—∫ 47.56 ± 1.36 mg
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