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¢ÿàπ‡ªìπμ—«∫àß™’È°“√‡®√‘≠¢Õ߇™◊ÈÕ√“ §à“§«“¡‡¢â¡¢âπ 6, 9, 12, 15, 18, 21, ·≈– 24 π”μ—«Õ¬à“߇擖
μË” ÿ¥∑’Ë “¡“√∂¬—∫¬—Èß°“√‡®√‘≠¢Õ߇™◊ÈÕ (Minimum ‡≈’Ȭß∑’ˇ°Á∫‰¥â¡“∑”°“√‡®◊Õ®“ß·≈â« spread ≈ß∫π
Inhibitory Concentration, MIC) μ—¥ ‘π®“° Õ“À“√ sabouraud dextrose agar ∫à¡∑’Ë 35 Õß»“
§«“¡‡¢â¡¢âπμË” ÿ¥∑’ˉ¡à¡’°“√‡®√‘≠¢Õ߇™◊ÈÕ√“„πÀ≈ÿ¡ ‡´≈‡´’¬ ‡ªìπ‡«≈“ 24 ™—Ë«‚¡ß ®“°π—Èππ—∫®”π«π
‚§‚≈π’∑’ˇ°‘¥¢÷Èπ ·≈–«‘‡§√“–À姫“¡ —¡æ—π∏å√–À«à“ß
°“√∑¥ Õ∫ƒ∑∏‘Ï¶à“‡™◊ÈÕ√“
‡«≈“·≈–®”π«π¢Õ߇™◊ÈÕ∑’ˇÀ≈◊ÕÕ¬Ÿà
®“°°“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“ “¡“√∂π”¡“À“
º≈°“√»÷°…“
§à“§«“¡‡¢â¡¢âπμË” ÿ¥„π°“√¶à“‡™◊ÈÕ√“ (Minimum
Fungicidal Concentration, MFC) ‰¥â‚¥¬π” °“√ °—¥‡Àßâ“¢Õß ¡ÿπ‰æ√«ß»å¢‘ß∑’Ë¡’°“√„™â·≈–
Õ“À“√‡æ“–‡≈’Ȭ߮“°À≈ÿ¡∑’ˉ¡à¡’°“√‡®√‘≠¢Õ߇™◊ÈÕ¡“ ®”Àπà“¬∑—Ë«‰ª®”π«π 15 ™π‘¥ ¥â«¬‡Õ∑“πÕ≈ ‰¥â
spread ≈ß∫πÕ“À“√ sabouraud dextrose agar ª√‘¡“≥ “√ °—¥∑’˧”π«≥®“°πÈ”Àπ—°·ÀâߢÕ߇Àßâ“
𔉪‡æ“–‡≈’Ȭß∑’ËÕÿ≥À¿Ÿ¡‘ 35 Õß»“‡´≈‡´’¬ ‡ªìπ‡«≈“ ¡ÿπ‰æ√¥—ßπ’È (% yields, w/w) °√–™“¬ (4.97%)
2 «—π ”À√—∫‡™◊ÈÕ¬’ μå ·≈– 4 - 7 «—π ”À√—∫‡™◊ÈÕ√“ °√–™“¬¥” (2.77%) °√–∑◊Õ (14.58%) ¢¡‘Èπ¢“«
·∫∫¡’‡ âπ„¬ —߇°μ°“√‡®√‘≠¢Õ߇™◊ÈÕ√“∫πÕ“À“√ (1.71%) ¢¡‘Èπ¢“«ªÉ“ (4.35) ¢¡‘Èπ™—π (10.09%) ¢¡‘Èπ
§à“§«“¡‡¢â¡¢âπμË” ÿ¥∑’Ë “¡“√∂¶à“‡™◊ÈÕ√“μ—¥ ‘π®“° ¥” (3.51%) ¢¡‘ÈπÕâÕ¬ (11.71%) ¢à“ (11.05%) ¢‘ß
§«“¡‡¢â¡¢âπ∑’ˉ¡àæ∫°“√‡®√‘≠¢Õ߇™◊ÈÕ√“∫πÕ“À“√ (4.06%) ‡ª√“–ÀÕ¡ (3.61%) ‰æ≈ (10.45%) ‰æ≈¥”
‡æ“–‡≈’È¬ß (4.06%) «à“π™—°¡¥≈Ÿ° (9.65%) ·≈–«à“ππ“ߧ”
(8.20%) ·≈–‡¡◊ËÕπ”¡“∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“‚¥¬«‘∏’
°“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“¢Õß “√ °—¥ ¡ÿπ‰æ√
broth microdilution assay „π™à«ß§«“¡‡¢â¡¢âπ
μàÕÀπ૬‡«≈“
1.56 - 100 ‰¡‚§√°√—¡μàÕ¡‘≈≈‘≈‘μ√ æ∫«à“ “√ °—¥
°“√»÷°…“ time - kill curve ¢Õß “√μâ“π‡™◊ÈÕ√“ ®“°‡Àßâ“ ¡ÿπ‰æ√ 7 ™π‘¥ §◊Õ ¢¡‘Èπ¢“«ªÉ“ ¢¡‘Èπ™—π
∑”‚¥¬‡μ√’¬¡ “√ °—¥ ¡ÿπ‰æ√·≈– ketoconazole ¢¡‘Èπ¥” ¢¡‘ÈπÕâÕ¬ ‰æ≈¥” «à“π™—°¡¥≈Ÿ° ·≈–«à“π
„πÕ“À“√ RPMI-MOPS „Àâ¡’§«“¡‡¢â¡¢âπ¢Õß “√ π“ߧ” · ¥ßƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“μàÕ‡™◊ÈÕ°≈ÿࡇ¥Õ√å¡“‚μ-
‡ªìπ Õ߇∑à“¢Õߧ«“¡‡¢â¡¢âπ∑’ËμâÕß°“√»÷°…“ §◊Õ MIC ‰øμå∑—Èß 3 ™π‘¥∑’Ë„™â∑¥ Õ∫∑’Ë MICs ·≈– MFCs
·≈– MFC ª√‘¡“μ√ 1 ¡‘≈≈‘≈‘μ√ ®“°π—Èπ‡μ√’¬¡‡™◊ÈÕ √–À«à“ß 12.50 - 100 ‰¡‚§√°√—¡μàÕ¡‘≈≈‘≈‘μ√ ·μà‰¡à
C. albicans „Àâ¡’§«“¡‡¢â¡¢âπ¢Õ߇™◊ÈÕ‡∑à“°—∫ 0.5 · ¥ßƒ∑∏‘Ïμâ“π‡™◊ÈÕ C. albicans ∑’˧«“¡‡¢â¡¢âπμË”°«à“
McFarland standard ·≈⫇®◊Õ®“߇™◊ÈÕ∑’ˉ¥â¥â«¬ 100 ‰¡‚§√°√—¡μàÕ¡‘≈≈‘≈‘μ√ ¬°‡«âπ “√ °—¥®“°
RPMI-MOPS „πÕ—μ√“ à«π 1 : 500 º ¡‡™◊ÈÕ∑’Ë ‡Àßâ“ ¡ÿπ‰æ√ 2 ™π‘¥ §◊Õ ¢¡‘Èπ¢“«ªÉ“·≈–‰æ≈¥” (√Ÿª
‡μ√’¬¡‰¥âª√‘¡“μ√ 1 ¡‘≈≈‘≈‘μ√ ≈ß„π “√μâ“π‡™◊ÈÕ√“ ∑’Ë 1) ∑’Ë· ¥ßƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“‰¥â¥’μàÕ‡™◊ÈÕ√“∑’Ë„™â
∑’ˇμ√’¬¡‰«â „™â DMSO ‡ªìπ negative control ∑¥ Õ∫∑—Èß 4 ™π‘¥ ‚¥¬¡’ƒ∑∏‘Ïμâ“π‡™◊ÈÕ C. albicans
·≈â«∫ࡇ擖‡≈’Ȭ߇™◊ÈÕ∑’ËÕÿ≥À¿Ÿ¡‘ 35 Õß»“‡´≈‡´’¬ ∑’Ë MICs 25 ·≈– 50 ‰¡‚§√°√—¡μàÕ¡‘≈≈‘≈‘μ√μ“¡≈”¥—∫
∑”°“√‡°Á∫μ—«Õ¬à“߇擖‡≈’Ȭ߄π™—Ë«‚¡ß∑’Ë 0, 1, 2, 3, ·≈–· ¥ßƒ∑∏‘Ï¶à“‡™◊ÈÕ√“ C. albicans ∑’Ë MFCs 100
