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‡°Á∫®“°®—ßÀ«—¥√“™∫ÿ√’√–À«à“ߪï 2556 - 2557 ‡°Á∫ 0.85% sodium chloride (NaCl) ‡æ◊ËÕ„Àâ¡’§«“¡Àπ“
6
√—°…“μ—«Õ¬à“ßæ√√≥‰¡âÕâ“ßՑ߇æ◊ËÕß“π«‘®—¬‰«â∑’Ë ·πàπ¢Õ߇™◊ÈÕ 1 - 5 × 10 ‡´≈≈åμàÕ¡‘≈≈‘≈‘μ√ ·≈–¡’
”π—°ß“πÀÕæ√√≥‰¡â °√¡Õÿ∑¬“π·Ààß™“μ‘ —μ«åªÉ“ §«“¡¢ÿàπ‡¡◊ËÕ«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë 530 π“‚π
·≈–æ—π∏ÿåæ◊™ °√ÿ߇∑æœ ‡¡μ√‡∑à“°—∫ 0.5 McFarland standard ”À√—∫

‡™◊ÈÕ¬’ μå·≈– 1 McFarland standard ”À√—∫
°“√‡μ√’¬¡ “√ °—¥‡Õ∑“πÕ≈®“°‡Àßâ“ ¡ÿπ‰æ√
‡™◊ÈÕ√“·∫∫¡’‡ âπ„¬ ®“°π—Èπ‡®◊Õ®“߇™◊ÈÕÕ’°§√—Èߥ⫬
à«π¢Õ߇Àßâ“ ¡ÿπ‰æ√ ¥∂Ÿ°À—Ëπ„Àâ¡’¢π“¥‡≈Á° Õ“À“√ RPMI-MOPS (RPMI 1640 medium,

·≈–º÷Ëß„Àâ·Àâß∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕß π” ¡ÿπ‰æ√·Àâß without sodium bicarbonate ∑’Ë¡’ à«πª√–°Õ∫¢Õß
ª√–¡“≥ 10 - 20 °√—¡ ¡“À¡—°¥â«¬‡Õ∑“πÕ≈‡ªìπ 2 mML-glutamine ·≈– 165 mM morpholinepro-

√–¬–‡«≈“Õ¬à“ßπâÕ¬ 1 §◊π „πÕ—μ√“ à«π 1 °√—¡ panesulfonic acid (MOPS) ) „πÕ—μ√“ à«π 1:500
¢Õß ¡ÿπ‰æ√·ÀâßμàÕ‡Õ∑“πÕ≈ 20 ¡‘≈≈‘≈‘μ√ ‚¥¬ ·≈– 1:50 μ“¡≈”¥—∫
∑”°“√ °—¥´È” 2 §√—Èß ®“°π—Èππ” “√ °—¥‡Õ∑“πÕ≈
°“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“‚¥¬«‘∏’ Broth
¡“√–‡À¬·Àâß¿“¬„μâ ÿ≠≠“°“»∑’ËÕÿ≥À¿Ÿ¡‘ 37 Õß»“
microdilution assay
‡´≈‡´’¬ π” “√ °—¥À¬“∫∑’ˉ¥â¡“‡μ√’¬¡μ—«Õ¬à“ß

”À√—∫°“√∑¥ Õ∫ƒ∑∏‘Ïμâ“π‡™◊ÈÕ√“ ‚¥¬≈–≈“¬ “√ °“√∑¥ Õ∫„™â«‘∏’°“√∑’Ë¥—¥·ª≈ß¡“®“°«‘∏’°“√
°—¥À¬“∫¥â«¬ dimethyl sulfoxide (DMSO) „Àâ μ“¡ National Committee for Clinical Labora-
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‰¥â§«“¡‡¢â¡¢âπ 10 ¡‘≈≈‘°√—¡μàÕ¡‘≈≈‘≈‘μ√ ·≈–‡°Á∫ tory Standards (NCCLS) guideline ‚¥¬
√—°…“∑’ËÕÿ≥À¿Ÿ¡‘ -20 Õß»“‡´≈‡´’¬ ®π°«à“®–π”¡“ ‡μ√’¬¡ “√ °—¥ ¡ÿπ‰æ√ ”À√—∫∑¥ Õ∫„πÕ“À“√
∑¥ Õ∫ RPMI-MOPS „Àâ¡’§«“¡‡¢â¡¢âπ‡ªìπ Õ߇∑à“¢Õß
§«“¡‡¢â¡¢âπ∑’ËμâÕß°“√»÷°…“ ‡μ√’¬¡‡ªìπ two-fold
‡™◊ÈÕ®ÿ≈™’æ
serial dilution ª√‘¡“μ√ 50 ‰¡‚§√≈‘μ√ „π 96-well
‡™◊ÈÕ√“∑’Ë„™â»÷°…“‰¥â®“° ∂“∫—π«‘®—¬«‘∑¬“»“ μ√å culture plate ™π‘¥ flat bottom ”À√—∫°“√
“∏“√≥ ÿ¢ °√¡«‘∑¬“»“ μ√å°“√·æ∑¬å ª√–°Õ∫¥â«¬ ∑¥ Õ∫‡™◊ÈÕ¬’ μå·≈–„™â plate ™π‘¥ U-shape wells

‡™◊ÈÕ¬’ μå 1 ™π‘¥ §◊Õ Candida albicans DMST ”À√—∫°“√∑¥ Õ∫‡™◊ÈÕ√“·∫∫¡’‡ âπ„¬ ∑”°“√∑¥ Õ∫
5815 ·≈–‡™◊ÈÕ√“·∫∫¡’‡ âπ„¬°≈ÿࡇ¥Õ√å¡“‚μ‰øμå 3 μ—«Õ¬à“ß “√ °—¥ ¡ÿπ‰æ√·μà≈–™π‘¥´È” 2 §√—Èß „™â

™π‘¥ §◊Õ Trichophyton rubrum DMST 30263, ketoconazole ‡ªìπ positive control ·≈–„™â DMSO
Trichophyton mentagrophytes DMST 19735, ‡ªìπ negative control „ à‡™◊ÈÕ∑’ˇμ√’¬¡‰«â ”À√—∫
·≈– Microsporum gypseum DMST 21146 ‚¥¬ °“√∑¥ Õ∫ª√‘¡“μ√ 50 ‰¡‚§√≈‘μ√≈ß„π·μà≈–À≈ÿ¡

∑”°“√‡æ“–‡≈’Ȭß∫π sabouraud dextrose agar ∫à¡∂“¥‡æ“–‡≈’Ȭß∑’ËÕÿ≥À¿Ÿ¡‘ 35 Õß»“‡´≈‡´’¬ ‡ªìπ
slants ·≈–‡°Á∫√—°…“∑’ËÕÿ≥À¿Ÿ¡‘ 2 - 8 Õß»“‡´≈‡´’¬ ‡«≈“ 24 ™—Ë«‚¡ß ”À√—∫‡™◊ÈÕ¬’ μå ·≈– 96 - 120

‚¥¬‡ª≈’ˬπÕ“À“√„À¡àÕ¬à“ßπâÕ¬∑ÿ° 2 —ª¥“Àå ‡μ√’¬¡ ™—Ë«‚¡ß ”À√—∫‡™◊ÈÕ√“·∫∫¡’‡ âπ„¬ À≈—ß®“°§√∫
‡™◊ÈÕ ”À√—∫°“√∑¥ Õ∫‚¥¬‡®◊Õ®“߇™◊ÈÕ·μà≈–™π‘¥„π °”Àπ¥°“√∫ࡇ≈’È¬ß Õà“πº≈°“√∑¥ Õ∫‚¥¬¥Ÿ§«“¡