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Abstract
The effect of crude extract from Derris scandens (Roxb.) Benth. on Aedes aegypti larvaes
with community participation in Phetchabun province
Benchaporn Srisuvoramas*, Suwit Wunnasri*, Saowapa Chumanee*, Sonthaya Puengsiri*,
†
†
Jerasak Tappha , Jarungjitr Tappha , Nikom Jantaramungkorn ‡
†
*Faculty of Science and Technology, Phetchabun Rajabhat University, Vector Born Disease Control
‡
Center 9.2, Phetchabun Province, Faculty of Management Science, Phetchabun Rajabhat University
This study used three experiments with crude extracts of Derris scandens (thaowanpriang
in Thai): first, using water crude extract; second, using dichloromethane crude extract; and third,
using 95% ethyl alcohol extract. Three concentrations of D. scandens crude extracts were used in
each experiment, i.e. 200 ppm, 250 ppm and 300 ppm, respectively. The study tested the crude
extract with four stages of Aedes aegypti larvae. It was found in laboratory that only the alcohol
extract of D. scandens had larvicidal effects on A. aegypti larvae; every concentration could kill
all (100%) of first-stage larvae. The LC values were 250 ppm for second-stage larvae and 300
50
ppm for third-stage larvae. All concentrations had no effect on fourth stage larvae. With the
AOAC technique, it was found that D. scandens extract had no toxicity from any heavy metals
and carcinogens. When using the D. scandens extract in the field, it was found that the 250 ppm
solution could not kill any larvae, but the 1,000 ppm solution could kill 83.33% of larvae. After
the field experiment, the Aedes larval indices (HI, CI and BI) dropped significantly (p = 0.05),
compared with before the experiment; and the people involved in the field experiment had a
x
x
x
moderate level of satisfaction with the results (x x = 2.63 ± 0.29).
Key words: Derris scandens (Roxb.) Benth.; Aedes aegypti larvae; participatory action research
(PAR)