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             ∑’Ë¡’§«“¡‡¢â¡¢âπ√âÕ¬≈–‚¥¬¡«≈μàÕª√‘¡“μ√ 7.5  „π “√≈–≈“¬øÕ ‡øμ∫—ø‡øÕ√姫“¡‡¢â¡¢âπ 0.2

             ª√‘¡“μ√ 20 ‰¡‚§√≈‘μ√ ∑‘È߉«â∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕß ‡ªìπ‡«≈“  ¡‘≈≈‘‚¡≈“√å „ à≈ß„π 96 well plates À≈ÿ¡≈– ª√‘¡“μ√
             30 π“∑’ ®“°π—Èπ𔉪«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë   100 ‰¡‚§√≈‘μ√º ¡°—∫ “√≈–≈“¬ L-DOPA ∑’Ë

             §«“¡¬“«§≈◊Ëπ 750 π“‚π‡¡μ√ π”§à“°“√¥Ÿ¥°≈◊π   §«“¡‡¢â¡¢âπ 2.5 ¡‘≈≈‘‚¡≈“√å ª√‘¡“μ√ 50 ‰¡‚§√≈‘μ√
             · ß∑’ˉ¥â ‰ª§”π«≥À“ª√‘¡“≥ “√ª√–°Õ∫øïπÕ≈‘°   ‡μ‘¡‡Õπ‰´¡å‰∑‚√´‘‡π ∑’˧«“¡‡¢â¡¢âπ 125 Àπ૬/
             ∑—ÈßÀ¡¥‡©≈’ˬ„π√Ÿª¡‘≈≈‘°√—¡¢Õß°√¥·°≈≈‘°μàÕ°√—¡  ¡‘≈≈‘≈‘μ√ ª√‘¡“μ√ 50 ‰¡‚§√≈‘μ√ º ¡„À⇢⓰—π

             (¡‘≈≈‘°√—¡/°√—¡) ‡∑’¬∫®“°°√“ø¡“μ√∞“π√–À«à“ß  ·≈–∫à¡∑’ËÕÿ≥À¿Ÿ¡‘ 37 Õß»“‡´≈‡´’¬  ‡ªìπ‡«≈“ 15 π“∑’
             §«“¡‡¢â¡¢âπ¢Õß°√¥·°≈≈‘°·≈–§à“¥Ÿ¥°≈◊π· ß     ®“°π—Èπ𔉪«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’Ë™à«ß§«“¡¬“«

             ‚¥¬∑”°“√∑¥ Õ∫μ—«Õ¬à“ß≈– 3 §√—Èß             §≈◊Ëπ 490 π“‚π‡¡μ√ ¥â«¬‡§√◊ËÕß«—¥§à“°“√¥Ÿ¥°≈◊π
                 5.2 «‘‡§√“–À姫“¡ “¡“√∂„π°“√μâ“πÕπÿ¡Ÿ≈  · ß·∫∫‰¡‚§√‡æ≈∑ ∑”°“√§”π«≥√âÕ¬≈–°“√
                                                 [9]
             Õ‘ √– (DPPH radical scavenging activity)    ¬—∫¬—È߇Õπ‰´¡å‰∑‚√´‘‡π  (%Inhibition) ¥—ß ¡°“√
                 ‡μ√’¬¡ “√ °—¥‰À¡¢â“«‚楷≈– “√¡“μ√∞“π    %Inhibition = (A    - A   )/ A     x 100
                                                                       §«∫§ÿ¡  ∑¥ Õ∫   §«∫§ÿ¡
             α-Tocopherol (positive control) ∑’˧«“¡‡¢â¡¢âπ
                                                         7. °“√«‘‡§√“–Àå¢âÕ¡Ÿ≈
             μ—Èß·μà 0-250 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ (Two-fold dilu-

             tion) ≈–≈“¬„π‡Õ∑“πÕ≈ „ à≈ß„π 96 well plates     °“√»÷°…“·μà≈–§√—Èß ∑”°“√∑¥≈Õß 3 ´È” π”§à“∑’Ë
             À≈ÿ¡≈– 30 ‰¡‚§√¡‘≈≈‘≈‘μ√ ·≈â«‡μ‘¡ “√≈–≈“¬   ‰¥â¡“À“§à“∑“ß ∂‘μ‘ ‰¥â·°à §à“‡©≈’ˬ‡∫’ˬ߇∫π¡“μ√∞“π
             DPPH ∑’Ë¡’§«“¡‡¢â¡¢âπ 0.5 ¡‘≈≈‘‚¡≈“√å ª√‘¡“μ√ 150  §à“§«“¡·ª√ª√«π ·≈–«‘‡§√“–À姫“¡·ª√ª√«π

             ‰¡‚§√≈‘μ√ ∑‘È߉«â∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕß ‡ªìπ‡«≈“ 30 π“∑’  (Analysis of Variance: ANOVA) ∑’Ë√–¥—∫§«“¡
             ®“°π—Èπ𔉪«—¥§à“°“√¥Ÿ¥°≈◊π· ß∑’˧«“¡¬“«§≈◊Ëπ  ‡™◊ËÕ¡—Ëπ 95 ‡ªÕ√凴Áπμå (p  ≤ 0.05) ¥â«¬‚ª√·°√¡

             595 π“‚π‡¡μ√ π”§à“∑’ˉ¥â‰ª§”π«≥√âÕ¬≈–°“√     ”‡√Á®√Ÿª Statistics Package for the Social Sci-
             ¬—∫¬—ÈßÕπÿ¡Ÿ≈Õ‘ √– DPPH (%inhibition) ¥—ß ¡°“√  ences (SPSS) version 17.0
             ·≈â«π”§à“§«“¡‡¢â¡¢âπ·≈–√âÕ¬≈–°“√¬—∫¬—ÈßÕπÿ¡Ÿ≈

             Õ‘ √– DPPH‡æ◊ËÕÀ“§«“¡ —¡æ—π∏凙‘߇ âπ (y = ax +          º≈°“√»÷°…“
                2
             b; r  linear regression) ·≈–‡∑’¬∫À“§à“ IC  %in-
                                               50
                                                         1. º≈°“√‡μ√’¬¡ “√ °—¥‰À¡¢â“«‚æ¥
             hibition = (A    - A     )/A     x 100
                        §«∫§ÿ¡    “√ °—¥  §«∫§ÿ¡
                                                             æ∫«à“ “√ °—¥‰À¡¢â“«‚楄Àâ√âÕ¬≈–ª√‘¡“≥
                                                 [10]
             6. °“√»÷°…“ƒ∑∏‘Ϭ—∫¬—È߇Õπ‰´¡å‰∑‚√´‘‡π 
                                                          “√ °—¥·μ°μà“ß°—πÕ¬à“ß¡’π—¬ ”§—≠∑“ß ∂‘μ‘ (p =
                 «‘‡§√“–À姫“¡ “¡“√∂„π°“√¬—∫¬—È߇Õπ‰´¡å‰∑‚√  0.01) ‚¥¬∑’ˉÀ¡¢â“«‚楢⓫‡À𒬫„Àâª√‘¡“≥ “√
             ´‘‡π  (Tyrosinase inhibition activity) ‡μ√’¬¡   °—¥ Ÿß ÿ¥ √âÕ¬≈– 6.88 ± 0.02 √Õß≈ß¡“§◊Õ ‰À¡
              “√ °—¥‰À¡¢â“«‚楷≈– “√¡“μ√∞“π°√¥‚§®‘°      ¢â“«‚æ¥À«“π„Àâª√‘¡“≥ “√ °—¥ √âÕ¬≈– 6.44 ± 0.01

             (positive control) ∑’˧«“¡‡¢â¡¢âπμ—Èß·μà 0-500  ·≈–‰À¡¢â“«‚æ¥À«“π ≈—∫ ’„Àâª√‘¡“≥ “√ °—¥μË” ÿ¥
             ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ (Two-fold dilution) ≈–≈“¬  √âÕ¬≈– 4.23 ± 0.01  ¥—ßμ“√“ß∑’Ë 2
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