Page 31 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 16 ฉบับที่ 3

365

‡´π쑇¡μ√ ·≈–¥â“π≈à“߇∑à“°—∫ 1 ‡´π쑇¡μ√ §«“¡ proximate analysis

°«â“ߢÕß°“√ spot ‡∑à“°—∫ 0.3 ‡´π쑇¡μ√ ·≈–
5. °“√»÷°…“ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–
√–¬–Àà“ߢÕß°“√ spot ‡∑à“°—∫ 1 ‡´π쑇¡μ√

ª√‘¡“≥ “√∑’Ë spot ·∂∫≈– 20 ‰¡‚§√≈‘μ√ ∑’˧«“¡ 5.1 «‘‡§√“–ÀåÀ“ª√‘¡“≥øïπÕ≈‘°‚¥¬√«¡ (total
[8]
‡¢â¡¢âπ 10 ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ π”·ºàπ TLC „ à phenolic content)
¿“™π–ªî¥∑’Ë¡’μ—«∑”≈–≈“¬ (Mobile phase) ¿“«– ‡μ√’¬¡ “√ °—¥‰À¡¢â“«‚楷≈–°√¥·°≈≈‘°

∑’Ë “¡“√∂·¬°°≈ÿà¡¢Õß “√ ∑’Ëæ∫„π “√ °—¥‰À¡ ( “√¡“μ√∞“π) ∑’˧«“¡‡¢â¡¢âπ 0, 20, 40, 60, 80 ·≈–
¢â“«‚楉¥â¥’ §◊Õ ethanol:methanol „πÕ—μ√“ à«π 100 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ „π‡Õ∑“πÕ≈ „ à≈ß„π 96

2:1 ·≈â«π”‰ªæàπ¥â«¬ reagents ‡æ◊ËÕμ√«®À“Õߧå well plates À≈ÿ¡≈– 80 ‰¡‚§√¡‘≈≈‘≈‘μ√ ‡μ‘¡ “√
ª√–°Õ∫∑“߇§¡’‡∫◊ÈÕßμâπ„π “√ °—¥‰À¡¢â“«‚æ¥ ≈–≈“¬ Folin-Cioculteu ∑’˧«“¡‡¢â¡¢âπ 1 πÕ√å¡Õ≈
ª√‘¡“μ√ 100 ‰¡‚§√≈‘μ√ ·≈– “√≈–≈“¬ Na CO
2 3
4. °“√»÷°…“§ÿ≥§à“∑“ß‚¿™π“°“√¢Õßμ—«Õ¬à“ß
[7]
‰À¡¢â“«‚æ¥ Standard Curve (Gallic Acid)
Absorbance (750 nm)
π”μ—«Õ¬à“߉À¡¢â“«‚æ¥ÕÕ°®“°∑’ˇ°Á∫√—°…“ ´÷Ëß

ºà“π°“√≈â“ß∑”§«“¡ –Õ“¥·≈–Õ∫∑’ËÕÿ≥À¿Ÿ¡‘ 50
Õß»“‡´≈‡´’¬ π“π 20 ™—Ë«‚¡ß °àÕπ‡°Á∫√—°…“„πμŸâ
‡¬Áπ∑’ËÕÿ≥À¿Ÿ¡‘ 4 Õß»“‡´≈‡´’¬ √Õ„ÀâÕÿ≥À¿Ÿ¡‘

μ—«Õ¬à“߇∑à“°—∫Õÿ≥À¿Ÿ¡‘ÀâÕß π”μ—«Õ¬à“ß ÕÕ°®“°∂ÿß Concentration (μg/ml)
æ≈“ μ‘° ·≈–«‘‡§√“–Àå§ÿ≥ ¡∫—μ‘μà“ß Ê ¥â«¬«‘∏’ √Ÿª∑’Ë 1 ¡“μ√∞“π¢Õß°√¥·°≈≈‘°

√Ÿª∑’Ë 2 °“√∑¥ Õ∫°“√·¬°°≈ÿà¡ “√