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              ‡´π쑇¡μ√ ·≈–¥â“π≈à“߇∑à“°—∫ 1 ‡´π쑇¡μ√ §«“¡  proximate analysis

              °«â“ߢÕß°“√ spot ‡∑à“°—∫ 0.3 ‡´π쑇¡μ√ ·≈–
                                                          5. °“√»÷°…“ƒ∑∏‘Ïμâ“πÕπÿ¡Ÿ≈Õ‘ √–
              √–¬–Àà“ߢÕß°“√ spot ‡∑à“°—∫ 1 ‡´π쑇¡μ√

              ª√‘¡“≥ “√∑’Ë spot ·∂∫≈– 20 ‰¡‚§√≈‘μ√ ∑’˧«“¡    5.1 «‘‡§√“–ÀåÀ“ª√‘¡“≥øïπÕ≈‘°‚¥¬√«¡ (total
                                                                         [8]
              ‡¢â¡¢âπ 10 ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ π”·ºàπ TLC „ à  phenolic content)
              ¿“™π–ªî¥∑’Ë¡’μ—«∑”≈–≈“¬ (Mobile phase)  ¿“«–    ‡μ√’¬¡ “√ °—¥‰À¡¢â“«‚楷≈–°√¥·°≈≈‘°

              ∑’Ë “¡“√∂·¬°°≈ÿà¡¢Õß “√ ∑’Ëæ∫„π “√ °—¥‰À¡   ( “√¡“μ√∞“π) ∑’˧«“¡‡¢â¡¢âπ 0, 20, 40, 60, 80 ·≈–
              ¢â“«‚楉¥â¥’ §◊Õ ethanol:methanol „πÕ—μ√“ à«π  100 ‰¡‚§√°√—¡/¡‘≈≈‘≈‘μ√ „π‡Õ∑“πÕ≈ „ à≈ß„π 96

              2:1 ·≈â«π”‰ªæàπ¥â«¬ reagents ‡æ◊ËÕμ√«®À“Õß§å  well plates À≈ÿ¡≈– 80 ‰¡‚§√¡‘≈≈‘≈‘μ√ ‡μ‘¡ “√
              ª√–°Õ∫∑“߇§¡’‡∫◊ÈÕßμâπ„π “√ °—¥‰À¡¢â“«‚æ¥   ≈–≈“¬ Folin-Cioculteu ∑’˧«“¡‡¢â¡¢âπ 1 πÕ√å¡Õ≈
                                                          ª√‘¡“μ√ 100 ‰¡‚§√≈‘μ√ ·≈– “√≈–≈“¬ Na CO
                                                                                             2   3
              4. °“√»÷°…“§ÿ≥§à“∑“ß‚¿™π“°“√¢Õßμ—«Õ¬à“ß
                         [7]
              ‰À¡¢â“«‚æ¥                                               Standard Curve (Gallic Acid)
                                                           Absorbance (750 nm)
                   π”μ—«Õ¬à“߉À¡¢â“«‚æ¥ÕÕ°®“°∑’ˇ°Á∫√—°…“ ´÷Ëß

              ºà“π°“√≈â“ß∑”§«“¡ –Õ“¥·≈–Õ∫∑’ËÕÿ≥À¿Ÿ¡‘ 50
              Õß»“‡´≈‡´’¬  π“π 20 ™—Ë«‚¡ß °àÕπ‡°Á∫√—°…“„πμŸâ
              ‡¬Áπ∑’ËÕÿ≥À¿Ÿ¡‘ 4 Õß»“‡´≈‡´’¬  √Õ„ÀâÕÿ≥À¿Ÿ¡‘

              μ—«Õ¬à“߇∑à“°—∫Õÿ≥À¿Ÿ¡‘ÀâÕß π”μ—«Õ¬à“ß ÕÕ°®“°∂ÿß           Concentration (μg/ml)
              æ≈“ μ‘° ·≈–«‘‡§√“–Àå§ÿ≥ ¡∫—μ‘μà“ß Ê ¥â«¬«‘∏’       √Ÿª∑’Ë 1 ¡“μ√∞“π¢Õß°√¥·°≈≈‘°





























                                          √Ÿª∑’Ë 2 °“√∑¥ Õ∫°“√·¬°°≈ÿà¡ “√
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