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≈ß∫π·ºàπ TLC ¥â«¬‡§√◊ËÕß Sample application- ¡“°∑’Ë ÿ¥ §◊Õ √âÕ¬≈– 8.67 √Õß≈ß¡“ §◊Õ °“√‡°Á∫∑’Ë
CAMAG Linomat 5 ·≈â«®ÿà¡≈ß„π TLC tank ∑’Ë 72, 24, 1 ·≈– 0 ™—Ë«‚¡ß ´÷Ëß¡’ª√‘¡“≥ SG √âÕ¬≈–
‡μ√’¬¡‰«â √Õ®π “√‡§≈◊ËÕπ∑’ˉª®π∂÷ß®ÿ¥ ‘Èπ ÿ¥ ·≈â« 8.45, 7.65, 7.23 ·≈– 7.22 μ“¡≈”¥—∫
𔉪«‘‡§√“–Àå¥â«¬‡§√◊ËÕß TLC scanner-CAMAG 2. ≈—°…≥–∑“ß‚§√¡“‚μ°√“øï ‡¡◊ËÕ«‘‡§√“–Àå
Linomat 5 ‡æ◊ËÕÀ“æ◊Èπ∑’Ë„μâ°√“ø ·≈– √â“ß°√“ø ¥â«¬ TLC º≈∑’ˉ¥â®“°°“√μ√«® Õ∫¢Õß “√ °—¥
¡“μ√∞“π (√Ÿª∑’Ë 1) ∫Õ√–‡æÁ¥ ‡ª√’¬∫‡∑’¬∫°—∫ “√¡“μ√∞“π æ∫«à“¡’®ÿ¥
(2) ‡μ√’¬¡ “√ °—¥ ¡ÿπ‰æ√∫Õ√–‡æÁ¥ ∑’Ë ∑’Ë¡’√–¬–∑“ß°“√‡§≈◊ËÕπ∑’Ëμ√ß°—π §◊Õ¡’§à“ R ‡∑à“°—∫
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§«“¡‡¢â¡¢âπ 100 ¡‘≈≈‘°√—¡μàÕ¡‘≈≈‘≈‘μ√ ¥â«¬πÈ”
ª√“»®“°‰ÕÕÕπ ®“°π—Èπ ©’¥≈ß∫π·ºàπ TLC π”
‰ª®ÿà¡≈ß„π TLC Tank ∑’ˇμ√’¬¡‰«â ·≈–μ√«®À“
ª√‘¡“≥ “√¥â«¬‡§√◊ËÕß TLC scanner-CAMAG
Linomat 5 ¥â«¬°“√π”æ◊Èπ∑’Ë„μâ°√“ø·∑π„π Ÿμ√
¢Õß°√“ø¡“μ√∞“π ·≈–‡æ◊ËÕÀ“ª√‘¡“≥ SG ∑’Ëæ∫
„π “√ °—¥∫Õ√–‡æÁ¥ ·≈–«‘‡§√“–Àå∑“ß ∂‘μ‘¥â«¬
√Ÿª∑’Ë 2 ≈—°…≥–¢Õß‚§√¡“‚μ°√“øï ®“°«‘∏’ TLC ∑’Ë —߇°μ
ANOVA
¥â«¬ UV 254 π“‚π‡¡μ√ ¢Õß “√ °—¥∫Õ√–‡æÁ¥
À¡“¬‡≈¢ 1-3 ‡°Á∫π“π 0 ™—Ë«‚¡ß À¡“¬‡≈¢ 4-
º≈°“√»÷°…“
6 ‡°Á∫π“π 1 ™—Ë«‚¡ß À¡“¬‡≈¢ 7-9 ‡°Á∫π“π
1. ª√‘¡“≥ “√ °—¥À¬“∫ ®“°°“√π”∫Õ 24 ™—Ë«‚¡ß À¡“¬‡≈¢ 10-12 ‡°Á∫π“π 72
™—Ë«‚¡ß À¡“¬‡≈¢ 13-15 ‡°Á∫π“π 120 ™—Ë«‚¡ß
√–‡æÁ¥¡“ °—¥¥â«¬πÈ” ‚¥¬«‘∏’°“√μâ¡„™âºß∫Õ√–‡æÁ¥
À¡“¬‡≈¢ 16-18 “√¡“μ√∞“π SG
100 °√—¡μàÕπÈ” 1,000 ¡‘≈≈‘≈‘μ√ ∑’Ë Õÿ≥À¿Ÿ¡‘ 100
Õß»“‡´≈‡´’¬ 15 π“∑’ ·≈–‡°Á∫∑’ËÕÿ≥À¿Ÿ¡‘ 4 Õß»“
‡´≈‡´’¬ ∑’Ë√–¬–‡«≈“μà“ß Ê æ∫«à“∑’Ë√–¬–‡«≈“„π
°“√‡°Á∫ 120 ™—Ë«‚¡ß ¡’ª√‘¡“≥¢Õß “√ °—¥À¬“∫
√Ÿª∑’Ë 3 ≈—°…≥–¢Õß‚§√¡“‚μ°√“øï ®“°«‘∏’ TLC ∑’Ë —߇°μ
¥â«¬ UV 366 π“‚π‡¡μ√ ¢Õß “√ °—¥∫Õ√–‡æÁ¥
À¡“¬‡≈¢ 1-3 ‡°Á∫π“π 0 ™—Ë«‚¡ß À¡“¬‡≈¢ 4-
6 ‡°Á∫π“π 1 ™—Ë«‚¡ß À¡“¬‡≈¢ 7-9 ‡°Á∫π“π
24 ™—Ë«‚¡ß À¡“¬‡≈¢ 10-12 ‡°Á∫π“π 72
™—Ë«‚¡ß À¡“¬‡≈¢ 13-15 ‡°Á∫π“π 120 ™—Ë«‚¡ß
√Ÿª∑’Ë 1 °√“ø¡“μ√∞“π Syringin À¡“¬‡≈¢ 16-18 “√¡“μ√∞“π SG