Page 28 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 16 ฉบับที่ 1

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∑’ˇ°Á∫∑’Ë√–¬–‡«≈“·μ°μà“ß°—π ‚¥¬∑”°“√«‘‡§√“–Àå °√Õߥ⫬ ”≈’ ·≈–‡μ√’¬¡μ—«Õ¬à“ß 4 μ—«Õ¬à“ß ‰¥â·°à

¥â«¬«‘∏’ Thin Layer Chromatography (TLC) μ—«Õ¬à“ß∑’ˉ¥âÀ≈—ß®“°°“√μâ¡·≈⫇°Á∫‰«â 0, 1, 24, 72
‡æ◊ËÕμ√«® Õ∫ “√ ”§—≠‡™‘ߧÿ≥¿“æ ·≈–«‘∏’ Thin ·≈– 120 ™—Ë«‚¡ß ∑’ËÕÿ≥À¿Ÿ¡‘ 4 Õß»“‡´≈‡´’¬ „πμŸâ

Layer Chromatography Densitometer (TLC §«∫§ÿ¡Õÿ≥À¿Ÿ¡‘
Densitometer) ‡æ◊ËÕμ√«®À“ “√ ”§—≠‡™‘ߪ√‘¡“≥ 2. °“√∑” “√ °—¥À¬“∫„À⇢⡢âπ‚¥¬π”¬“μâ¡
‡æ◊ËÕ»÷°…“º≈¢Õß√–¬–‡«≈“„π°“√‡°Á∫¬“μâ¡ ¡ÿπ‰æ√ ¡“√–‡À¬‡æ◊ËÕ‡Õ“μ—«∑”≈–≈“¬ÕÕ° ‚¥¬°“√„™â§«“¡

μàÕª√‘¡“≥ syringin ·≈–‡ªìπ¢âÕ¡Ÿ≈ ”§—≠„π°“√ ‡¬Áπ ¥â«¬°“√√–‡À‘¥·Àâß ¥â«¬‡§√◊ËÕß freeze dryer
®
æ—≤𓬓μâ¡æ√âÕ¡¥◊Ë¡„Àâ¡’¡“μ√∞“πμàÕ‰ª„πÕπ“§μ √ÿàπ free zone ¬’ËÀâÕ Labconco ∑’ËÕÿ≥À¿Ÿ¡‘ -100

Õß»“‡´≈‡´’¬ ‡ªìπ‡«≈“ 24 ™—Ë«‚¡ß ®“°π—Èπ™—Ëß “√∑’Ë
√–‡∫’¬∫«‘∏’»÷°…“
‡À≈◊Õ®“°°“√√–‡À¬‡æ◊ËÕ𔉪§”π«≥À“ª√‘¡“≥ “√
«— ¥ÿÕÿª°√≥å ·≈– “√‡§¡’ °—¥À¬“∫ ·≈–‡°Á∫ “√ °—¥®“°∫Õ√–‡æÁ¥∑’ˉ¥â®“°

1. ·ºàπ TLC °“√√–‡À¬·Àâ߉«â∑’ËμŸâ·™à·¢ÁßÕÿ≥À¿Ÿ¡‘ -20 Õß»“
2. TLC Densitometer CAMAG (TLC ‡´≈‡´’¬
Scanner 3) 3. °“√«‘‡§√“–Àå≈—°…≥–∑“ß‚§√¡“‚μ°√“øï

3. TLC scanner-CAMAG Linomat 5 3.1 Thin Layer chromatography (TLC)
4. Chloroform liquid chromatography ‡μ√’¬¡·ºàπ TLC (Siliga gel 60 F254
grade (Pkc Instruments, ¢Õπ·°àπ) Aluminium Sheet) ¢π“¥ 20x20 ‡´π쑇¡μ√

5. Methanol liquid chromatography ‚¥¬μ—«∑”≈–≈“¬∑’Ë„™â‡ªìπ‡ø ‡§≈◊ËÕπ∑’Ë ‰¥â·°à
grade (Pkc Instruments, ¢Õπ·°àπ) §≈Õ‚√øÕ√å¡ ·≈–‡¡∑“πÕ≈ Õ—μ√“ à«π 8:2 ®“°π—Èπ

6. ∫Õ√–‡æÁ¥ π” “√ °—¥∑’ˉ¥â ·≈– “√¡“μ√∞“π §◊Õ SG ®ÿ¥≈ß
7. “√¡“μ√∞“π syringin (sigma) ∫π·ºàπ TLC √Õ„Àâ·Àâß∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕß ·≈â«®ÿà¡≈ß„π
«‘∏’°“√»÷°…“ ‡ªìπ°“√»÷°…“‡™‘ß∑¥≈Õß¡’¢—Èπ TLC tank ∑’ˇμ√’¬¡‰«â √Õ®π “√‡§≈◊ËÕπ∑’ˉª®π∂÷ß

μÕπ¥—ßπ’È ®ÿ¥ ‘Èπ ÿ¥∑’Ë°”Àπ¥‰«â ·≈â«π”‰ª«‘‡§√“–Àå¥â«¬‡§√◊ËÕß
1. °“√‡μ√’¬¡ “√ °—¥ ¡ÿπ‰æ√∫Õ√–‡æÁ¥ √—ß ’Õ—≈μ√Ⓣ«‚Õ‡≈μ∑’˧«“¡¬“«§≈◊Ëπ 254 ·≈– 366

‚¥¬„™â∫Õ√–‡æÁ¥®“°‚√ß欓∫“≈°“√·æ∑¬å·ºπ‰∑¬ π“‚π‡¡μ√ ·≈⫧”π«≥À“§à“√–¬–∑“ß∑’Ë “√‡§≈◊ËÕπ
À≈«ßªŸÉ·øÖ∫ ÿ¿—∑‚∑ ¡“∑”§«“¡ –Õ“¥ ·≈–π”‡∂“ ∑’ˉ¥â À√◊Õ retention factor (R ) ‡æ◊ËÕμ√«® Õ∫À“
f
¡“ —∫‡ªìπ™‘Èπ·∫∫À¬“∫¢π“¥ 3 ‡´π쑇¡μ√ Õ∫∑’Ë “√ SG „π “√ °—¥∫Õ√–‡æÁ¥

Õÿ≥À¿Ÿ¡‘ 50-55 Õß»“‡´≈‡´’¬ „™â‡«≈“ 3 «—π ·≈– 3.2 Thin layer chromatography Den-
∫¥„À⇪ìπºß≈–‡Õ’¬¥ ®“°π—Èπ™—Ëߺß∫Õ√–‡æÁ¥ 100 sitometer (TLC Densitometer)
°√—¡ „ àºâ“¢“«∫“ß„ àÀ¡âÕ¬“μâ¡ ¡ÿπ‰æ√ º ¡°—∫πÈ” (1) ‡μ√’¬¡ “√¡“μ√∞“π SG ∑’˧«“¡‡¢â¡¢âπ

1,000 ¡‘≈≈‘≈‘μ√ μ—ÈßÕÿ≥À¿Ÿ¡‘∑’Ë 100 Õß»“‡´≈‡´’¬ 15 1, 0.5, 0.25, 0.125 ·≈– 0.0625 ¡‘≈≈‘°√—¡μàÕ¡‘≈≈‘≈‘μ√
π“∑’ ‡¡◊ËÕ§√∫‡«≈“∑’Ë°”Àπ¥ μ—Èß∑‘È߉«â®πÕÿàπ ·≈â« ‚¥¬≈–≈“¬„ππÌÈ“ª√“»®“°‰ÕÕÕπ ®“°π—Èπ ©’¥ “√