Page 24 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 16 ฉบับที่ 1

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ª√–‡∑»‰∑¬ ¡’ª√‘¡“≥ 1.1-8.5% ‚¥¬πÈ”Àπ—° ´÷Ëß ¢Õß«‘∏’«‘‡§√“–À套߰≈à“« æ∫«à“ calibration curve

À“°‡ª√’¬∫‡∑’¬∫°—∫ª√‘¡“≥ eurycomanone ∑’Ë ¡’§«“¡‡ªìπ‡ âπμ√ß„π™à«ß§«“¡‡¢â¡¢âπ 0.06-0.36
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2
√–∫ÿ‰«â„π‡¿ —™μ”√—∫¢Õߪ√–‡∑»¡“‡≈‡´’¬ ‰¥â ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ ¡’§à“ coefficient (R ) ‡∑à“°—∫
°”Àπ¥ª√‘¡“≥ eurycomanone „π√“°ª≈“‰À≈- 0.9995 §à“ % recovery Õ¬Ÿà„π™à«ß 96-105% §à“
‡º◊Õ° „π™à«ß 0.8-1.5% ‚¥¬πÈ”Àπ—° ®–‡ÀÁπ‰¥â«à“ HORRAT ‡∑à“°—∫ 1.66-1.96 §à“ LOD ·≈– LOQ
μ—«Õ¬à“ß ¡ÿπ‰æ√√“°ª≈“‰À≈‡º◊Õ°∑’Ëæ∫„πª√–‡∑» ¡’§à“‡∑à“°—∫ 0.0106 ·≈– 0.0354 ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√

‰∑¬ ¡’ª√‘¡“≥ “√ eurycomanone Ÿß°«à“ ´÷Ëßß“π μ“¡≈”¥—∫ ®“°¢âÕ¡Ÿ≈¢â“ßμâπ· ¥ß„Àâ‡ÀÁπ«à“«‘∏’
«‘®—¬π’È “¡“√∂𔉪„™â‡ªìπ¢âÕ¡Ÿ≈„π°“√°”Àπ¥ «‘‡§√“–Àå∑’Ëæ—≤π“¢÷Èππ’È¡’§«“¡‡À¡“– ¡ “¡“√∂π”

ª√‘¡“≥ eurycomanone „π√“°ª≈“‰À≈‡º◊Õ°‰¥â ‰ª„™â‡ªìπ«‘∏’μ√«®«‘‡§√“–Àåª√‘¡“≥ eurycomanone
‚¥¬°”À𥮓°§à“‡©≈’ˬ¢Õߪ√‘¡“≥ eurycomanone „π√“°ª≈“‰À≈‡º◊Õ° ·≈– “¡“√∂𔉪 Ÿà°“√°”Àπ¥
„πμ—«Õ¬à“ß≈∫¥â«¬§à“‡∫’ˬ߇∫π¡“μ√∞“π ´÷Ëß¡’§à“‡∑à“°—∫ ‡°≥±åª√‘¡“≥ “√ ”§—≠„π√“°ª≈“‰À≈‡º◊Õ°μàÕ‰ª

1.83% ‡¡◊ËÕªí¥‡ªìπ‡≈¢∑»π‘¬¡ 1 μ”·Àπàß ‰¥â§à“ 1.8%
°‘μμ‘°√√¡ª√–°“»
‚¥¬πÈ”Àπ—° ¥—ßπ—Èπ „π«—μ∂ÿ¥‘∫√“°ª≈“‰À≈‡º◊Õ°§«√
¡’ª√‘¡“≥ eurycomanone ‰¡àπâÕ¬°«à“ 1.8% ‚¥¬ §≥–ºŸâ«‘®—¬¢Õ¢Õ∫§ÿ≥ 𓬪√–∂¡ ∑Õß»√’√—°…å

πÈ”Àπ—° ÀâÕߪؑ∫—μ‘°“√‡°…μ√ «π ¡ÿπ‰æ√°√¡«‘∑¬“-
»“ μ√å°“√·æ∑¬å ®—ßÀ«—¥®—π∑∫ÿ√’ ∑’Ë„À⧫“¡
¢âÕ √ÿª
Õπÿ‡§√“–À凰Á∫μ—«Õ¬à“ß ¡ÿπ‰æ√ ·≈– ∂“∫—π«‘®—¬
«‘∏’«‘‡§√“–Àåª√‘¡“≥ eurycomanone „π√“° ¡ÿπ‰æ√ °√¡«‘∑¬“»“ μ√å°“√·æ∑¬å∑’Ë π—∫ πÿπß∫
ª≈“‰À≈‡º◊Õ°¥â«¬‡§√◊ËÕß HPLC ∑’Ëæ—≤π“¢÷Èπ ‚¥¬ ª√–¡“≥„π°“√∑”«‘®—¬§√—Èßπ’È

°—¥ºß√“°ª≈“‰À≈‡º◊Õ° ¥â«¬ methanol ª√‘¡“μ√
‡Õ° “√Õâ“ßÕ‘ß
50 ¡‘≈≈‘≈‘μ√ ‚¥¬«‘∏’ reflux ‡ªìπ‡«≈“ 1 ™—Ë«‚¡ß
1. Tem Smitinand. Thai plant names. Bangkok: Office of
°√Õß·≈–π” “√ °—¥∑’ˉ¥â¡“√–‡À¬®π·Àâß®“°π—Èπ
the Forest Herbarium. Department of National Park;
≈–≈“¬°≈—∫·≈–ª√—∫ª√‘¡“μ√¥â«¬ 50% methanol 1980. p.148-9. (in Thai)
2. Boonyaprapat N, Chokchaicharoenporn O. Medicinal
®π§√∫ 10 ¡‘≈≈‘≈‘μ√ π” “√≈–≈“¬¥—ß°≈à“« ª√‘¡“μ√
Plants indigenous to Thailand. Bangkok: Prachachon;
20 ‰¡‚§√≈‘μ√ ©’¥‡¢â“ Ÿà√–∫∫‚§√¡“‚∑°√“øï¢Õß 2000. p.175-6. (in Thai)
® 3. Supcharoen P, Rueng-rangsri N, Deewisej K. Herbal in
HPLC ‚¥¬„™â§Õ≈—¡πå™π‘¥ Atlantis T3 C18,
central national park. 2nd ed. Nonthaburi: Thai
4.6x150 ¡‘≈≈‘‡¡μ√ ·≈–„™â πÈ”°≈—Ëπ ·≈– (acetoni- Traditional Medicine Development Foundation; 2006.
108 p. (in Thai)
trile μàÕ methanol Õ—μ√“ à«π 1:1) ‡ªìπ«—Ø¿“§
4. National List of Essential Medicines A.D.2011. Published
‡§≈◊ËÕπ∑’Ë Õ—μ√“°“√‰À≈ 1.5 ¡‘≈≈‘≈‘μ√/π“∑’ ·≈–
in Government Gazette, Vol. 2, (28 June 2011). (in Thai)
μ√«®«—¥¥â«¬ PDA ∑’˧«“¡¬“«§≈◊Ëπ 243 π“‚π‡¡μ√ 5. H. Yusuf, Mustofa, M. Agus Wijayanti, R. Asmah
Susidarti, P. Budi Setia Asih, Suryawati, et al. a new
®–ª√“°Ø peak ¢Õß eurycomanone ∑’Ë retention
quassinoid of four isolated compounds from extracts
time ‡∑à“°—∫ 13.98 π“∑’ º≈°“√∑¥ Õ∫§«“¡„™â‰¥â Eurycoma longifolia Jack roots and their in-vitro