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μ“√“ß∑’Ë 1 «—Ø¿“§‡§≈◊ËÕπ∑’Ë·∫∫ Gradient program
Õ—μ√“°“√‰À≈ Acetonitrile:Methanol
‡«≈“ (π“∑’) πÈ” (%)
(¡‘≈≈‘≈‘μ√/π“∑’) Õ—μ√“ à«π 1:1 (%)
0.0 1.5 90.0 10.0
6.0 1.5 90.0 10.0
14.0 1.5 85.0 15.0
15.0 1.5 0.0 100.0
20.0 1.5 0.0 100.0
21.0 1.5 90.0 10.0
Õ—μ√“°“√‰À≈ : 1.5 ¡‘≈≈‘≈‘μ√/π“∑’
°“√μ√«®«—¥ “√ : PDA ∑’˧«“¡¬“«§≈◊Ëπ 243
π“‚π‡¡μ√
ª√‘¡“μ√∑’Ë©’¥ : 20 ‰¡‚§√≈‘μ√
°“√∑¥ Õ∫§«“¡„™â‰¥â¢Õß«‘∏’«‘‡§√“–Àå∑“߇§¡’
1.5 §«“¡‡ªìπ‡ âπμ√ß (linearity) ·≈–
™à«ß°“√«‘‡§√“–Àå (range)
©’¥ “√≈–≈“¬ “√¡“μ√∞“π euryco- √Ÿª∑’Ë 7 Calibration curve ¢Õß “√≈–≈“¬¡“μ√∞“π
eurycomanone „π™à«ß§«“¡‡¢â¡¢âπ 0.06-0.36
manone ∑’Ë 6 √–¥—∫§«“¡‡¢â¡¢âπ μ—Èß·μà 0.06-0.36
¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√
¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ §«“¡‡¢â¡¢âπ≈– 3 ´È” ·≈–«‘‡§√“–Àå
3 §√—Èß ¥â«¬‡§√◊ËÕß HPLC π”§à“ peak area ·≈–
§«“¡‡¢â¡¢âπ¢Õß “√≈–≈“¬ eurycomanone ‰ª √â“ß ®”π«π 6 ´È” ·≈–«‘‡§√“–Àå spike sample √–¥—∫≈–
calibration curve º≈°“√»÷°…“ (√Ÿª∑’Ë 7) 3 ´È” ·μà≈–´È”∑” duplicate º≈°“√»÷°…“¥—ßμ“√“ß
®“°√Ÿª∑’Ë 7 æ∫«à“ calibration curve ¢Õß ∑’Ë 2
“√¡“μ√∞“π eurycomanone ¡’≈—°…≥–‡ªìπ‡ âπμ√ß *μ“¡ AOAC Peer-verified methods pro-
„π™à«ß§«“¡‡¢â¡¢âπ 0.06-0.36 ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ gram on policies and procedures, Arlington,
‚¥¬¡’§à“ coefficient (R2) ‡∑à“°—∫ 0.9995 VA, USA (1998)
1.6 §«“¡·¡àπ (accuracy) ·≈–§«“¡‡∑’Ë¬ß **°“√¬Õ¡√—∫§«“¡‡∑’Ë¬ß ª√–‡¡‘π®“°
(precision) HORRAT (< 2)
‡μ‘¡ “√¡“μ√∞“π (spike) ∑’˧«“¡‡¢â¡¢âπ 1.7 ¢’¥®”°—¥¢Õß°“√μ√«®æ∫ (Limit of
√–¥—∫μà“ß Ê „π™à«ß°“√„™âß“π 3 √–¥—∫ ≈ß„π sample Detection, LOD)
blank ·≈â««‘‡§√“–Àå sample blank (unspiked) æ∫«à“ LOD ¡’§à“‡∑à“°—∫ 0.0106 ¡‘≈≈‘°√—¡/
