Page 16 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 16 ฉบับที่ 1

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π”¡“≈â“ß∑”§«“¡ –Õ“¥ À—Ëπ‡ªìπ™‘Èπ Ê º÷Ëß„Àâ·Àâß

®“°π—Èππ”¡“Õ∫„πμŸâÕ∫√âÕπ∑’ËÕÿ≥À¿Ÿ¡‘ 50 Õß»“
‡´≈‡´’¬ π“π 3 «—ππ”¡“∫¥‡ªìπºß 𔉪ºà“π·√àß

‡∫Õ√å 180 ·≈⫇°Á∫„ ࢫ¥·°â« ’™“ªî¥ π‘∑ à«π
¡ÿπ‰æ√·Àâßπ”¡“Õ∫ „πμŸâÕ∫√âÕπ∑’ËÕÿ≥À¿Ÿ¡‘ 50
Õß»“‡´≈‡´’¬ ®π·Àâß ‡ªìπ‡«≈“ 1 ™—Ë«‚¡ß ‡æ◊ËÕ‰≈à

§«“¡™◊Èπ ·≈â«π”¡“∫¥ºà“π·√à߇∫Õ√å 180 ·≈⫇°Á∫
√Ÿª∑’Ë 2 ‚§√ß √â“ß∑“߇§¡’¢Õß Eurycomanone
„ ࢫ¥·°â« ’™“ªî¥ π‘∑ μ√«® Õ∫μ—«Õ¬à“ß∑’Ëπ”¡“
»÷°…“‚¥¬π”μ—«Õ¬à“ß ¡ÿπ‰æ√¡“μ√«®‡Õ°≈—°…≥å
∑“߇§¡’¥â«¬«‘∏’‚§√¡“‚∑°√“øï™π‘¥º‘«∫“ß ‚¥¬
[18]
°”Àπ¥§ÿ≥¿“æ„π¥â“π‡§¡’øî ‘° å ·μଗߡ‘‰¥â ‡ª√’¬∫‡∑’¬∫≈—°…≥–‚§√¡“‚∑·°√¡°—∫μ—«Õ¬à“ßÕâ“ßÕ‘ß
¡’°“√°”Àπ¥ª√‘¡“≥ “√ ”§—≠ °“√«‘®—¬§√—Èßπ’È¡’ (authentic)
§«“¡μâÕß°“√∑’Ë®–æ—≤π“«‘‡§√“–ÀåÀ“ª√‘¡“≥ “√ 2. “√¡“μ√∞“π
eurycomanone ¢Õß«—μ∂ÿ¥‘∫√“°ª≈“‰À≈‡º◊Õ° eurycomanone §«“¡∫√‘ ÿ∑∏‘Ï > 90%
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¥â«¬«‘∏’ HPLC ´÷Ë߇ªìπ«‘∏’∑’Ë„À⧫“¡∂Ÿ°μâÕß·¡à𬔠(Chromadex , Cannada)
–¥«° √«¥‡√Á«·≈–‡™◊ËÕ∂◊Õ‰¥â ‡æ◊ËÕ„™â‡ªìπ·π«∑“ß„π 3. μ—«∑”≈–≈“¬
°“√ª√–‡¡‘π§ÿ≥¿“æ«—μ∂ÿ¥‘∫√“°ª≈“‰À≈‡º◊Õ°∑’Ëæ∫ acetonitrile (HPLC grade), methanol

„πª√–‡∑»‰∑¬ ·≈–„™â„π°“√«‘®—¬·≈–æ—≤π“μ”√—∫ (HPLC grade), πÈ” (deionized water)
¬“·≈–º≈‘μ¿—≥±å®“° ¡ÿπ‰æ√∑’Ë¡’√“°ª≈“‰À≈‡º◊Õ° 4. ‡§√◊ËÕß¡◊Õ·≈–Õÿª°√≥å

‡ªìπ à«πº ¡ High Performance Liquid Chromato-
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graph - PDA detector (Water , USA)
√–‡∫’¬∫«‘∏’»÷°…“ Mettler Toledo Analytical Balance

Eyela NE-1 Rotary Evaporator
«— ¥ÿ
Retsch AS200 Basic Sieve

1. μ—«Õ¬à“ß√“°ª≈“‰À≈‡º◊Õ° water bath
√«∫√«¡«—μ∂ÿ¥‘∫ ¡ÿπ‰æ√ª≈“‰À≈‡º◊Õ° (√“°) 5. «— ¥ÿ«‘∑¬“»“ μ√å
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®”π«π 27 μ—«Õ¬à“ß „π™à«ß‡¥◊Õπ°ÿ¡¿“æ—π∏å 2557 ∂÷ß Atlantis T3 HPLC (C18, 4.6 × 150
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‡¡…“¬π 2559 ‡ªìπ ¡ÿπ‰æ√ ¥®“°·À≈àß∏√√¡™“μ‘ ‰¡‚§√‡¡μ√) column, 3 ‰¡‚§√‡¡μ√, Atlantis T3,
μ√«®√–∫ÿ™π‘¥„Àâ∑√“∫™◊ËÕ«‘∑¬“»“ μ√å∑’Ë∂Ÿ°μâÕßμ“¡ Guard column (C18, 4.6x20 ‰¡‚§√‡¡μ√) col-
À≈—°Õπÿ°√¡«‘∏“πæ◊™®”π«π 3 μ—«Õ¬à“ß (authentic) umn, 3 ‰¡‚§√‡¡μ√, Nylon filter syringe 0.45

·≈– ¡ÿπ‰æ√·Àâß®“°√â“π¢“¬¬“ ¡ÿπ‰æ√®“°¿“§ μm, 13 ‰¡‚§√‡¡μ√, PVDF membrane filter 0.45
μà“ß Ê „πª√–‡∑» ®”π«π 24 μ—«Õ¬à“ß μ—«Õ¬à“ß ¥ ‰¡‚§√‡¡μ√, °√–¥“…°√Õß ‡∫Õ√å 4 ·≈–‡§√◊ËÕß·°â«