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                 ‡¡◊ËÕ C §◊Õ §«“¡‡¢â¡¢âπ¢Õß “√„π spike   eurycomanone „π√“°ª≈“‰À≈‡º◊Õ°
                       1
             sample                                             1.1 ™π‘¥¢Õßμ—«∑”≈–≈“¬„π°“√ °—¥
                      C §◊Õ §«“¡‡¢â¡¢âπ¢Õß “√„π sample          º≈°“√»÷°…“æ∫«à“ ‡¡◊ËÕπ” “√≈–≈“¬∑’ˉ¥â
                       2
             blank                                       ®“°°“√ °—¥√“°ª≈“‰À≈‡º◊Õ°¥â«¬ 50% methanol,
                      C §◊Õ §«“¡‡¢â¡¢âπ¢Õß “√∑’Ë‡μ‘¡‰ª   methanol, ·≈– πÈ”°≈—Ëπ ¡“«‘‡§√“–Àå¥â«¬‡§√◊ËÕß
                       3
             (analyte)                                   HPLC æ∫«à“ æ◊Èπ∑’Ë„μâ°√“ø¢Õß peak  “√ euryco-

                    2.3 ¢’¥®”°—¥¢Õß°“√μ√«®æ∫ (Limit of   manone ∑’Ë retention time 13.9-14.2 π“∑’ ‚¥¬
             Detection, LOD)                             °“√ °—¥¥â«¬ methanol „Àâª√‘¡“≥ eurycomanone

                    LOD §”π«≥®“°®ÿ¥μ—¥¢Õß°√“ø             Ÿß ÿ¥ (√Ÿª∑’Ë 3) ·≈– “√≈–≈“¬∑’ˉ¥â®“°°“√ °—¥¥â«¬
             ¡“μ√∞“π∑’Ë„™â‡μ√’¬¡ sample blank ‡¡◊ËÕ°√“ø  hexane ·≈– dichloromethane ¡’°“√μ°μ–°Õπ
             ¡“μ√∞“π‚¥¬„™â spiked sample blank ∑’Ë 6     ¿“¬À≈—ß°“√μ—Èß∑‘È߉«â∑’ËÕÿ≥À¿Ÿ¡‘ 25 Õß»“‡´≈‡´’¬ 

             √–¥—∫§«“¡‡¢â¡¢âπ¢Õß “√¡“μ√∞“π √–¥—∫≈– 3 ´È”  ¿“¬„π‡«≈“‰¡à‡°‘π 1 ™—Ë«‚¡ß ´÷Ë߇ªìπμ–°Õπ∑’˧ß∑π
             ®“°π—Èπ§”π«≥§à“‡∫’ˬ߇∫π¡“μ√∞“π¢Õß y-intercept  ‰¡à≈–≈“¬‡¡◊ËÕμ—Èß∑‘È߉«âπ“π 24 ™—Ë«‚¡ß
             (Sy/x) ·≈–§”π«≥§à“ LOD ‚¥¬§à“ LOD §◊Õ§à“        ¥—ßπ—Èπ „π°“√æ—≤π“«‘∏’«‘‡§√“–Àåª√‘¡“≥

             §«“¡‡¢â¡¢âπ∑’Ë response °—∫ y-intercept + 3Sy/x  eurycomanone „π√“°ª≈“‰À≈‡º◊Õ° ®÷߇≈◊Õ°„™â
                    2.4 ¢’¥®”°—¥¢Õß°“√«—¥‡™‘ߪ√‘¡“≥ (Limit  methanol ‡ªìπμ—«∑”≈–≈“¬Õ‘π∑√’¬å„π°“√ °—¥
             of Quantitation, LOQ)                       μ—«Õ¬à“ß√“°ª≈“‰À≈‡º◊Õ°

                    LOQ §”π«≥‚¥¬ LOQ ¡’§à“‡∑à“°—∫ 10            1.2 √–¬–‡«≈“∑’ˇÀ¡“– ¡„π°“√ °—¥
             ‡∑à“¢Õß Sy/x                                       º≈°“√»÷°…“æ∫«à“ ‡¡◊ËÕ °—¥μ—«Õ¬à“ß√“°

                    2.5 §«“¡®”‡æ“– (specificity)         ª≈“‰À≈‡º◊Õ° πÈ”Àπ—° 2 °√—¡ ‚¥¬°“√ reflux ¥â«¬
                    «‘‡§√“–Àå method blank ‚¥¬ °—¥·≈–    methanol ‡ªìπ‡«≈“ 0.5, 1, ·≈– 2 ™—Ë«‚¡ß ·≈â«
             «‘‡§√“–Àåμ“¡«‘∏’∑’Ëæ—≤π“ „™â “√‡§¡’∑—ÈßÀ¡¥ ·μà‰¡à¡’  π”¡“«‘‡§√“–Àå¥â«¬‡§√◊ËÕß HPLC æ∫«à“æ◊Èπ∑’Ë„μâ

             μ—«Õ¬à“߇æ◊ËÕμ√«® Õ∫ “√√∫°«π∑’ËÕ“®¡“®“° “√  °√“ø¢Õß peak  “√ eurycomanone ∑’Ë retention
             ‡§¡’À√◊ÕÕÿª°√≥å∑’Ë„™â                       time 13.84-14.09 π“∑’ ∑—Èß°“√ °—¥¥â«¬√–¬–‡«≈“

                 3. °“√«‘‡§√“–Àåª√‘¡“≥ eurycomanone „π   1 ·≈– 2 ™—Ë«‚¡ß¡’§à“‰¡à·μ°μà“ß°—π (√Ÿª∑’Ë 4)
             √“°ª≈“‰À≈‡º◊Õ°                                  ¥—ßπ—Èπ „π°“√æ—≤π“«‘∏’°“√«‘‡§√“–Àåª√‘¡“≥ “√
                 «‘‡§√“–Àåª√‘¡“≥ eurycomanone „π√“°      eurycomanone „π√“°ª≈“‰À≈‡º◊Õ° ®÷߇≈◊Õ°„™â

             ª≈“‰À≈‡º◊Õ° ®”π«π 27 μ—«Õ¬à“ß ¥â«¬«‘∏’∑’Ëæ—≤π“  methanol ‡ªìπμ—«∑”≈–≈“¬„π°“√ °—¥·≈–„™â‡«≈“
             ¢÷Èπ„π¢âÕ 1.4 (n=4)                         „π°“√ °—¥ 1 ™—Ë«‚¡ß
                                                                1.3 §«“¡¬“«§≈◊Ëπ„π°“√μ√«®«‘‡§√“–Àå “√
                           º≈°“√»÷°…“
                                                         eurycomanone
                 1. °“√æ—≤π“«‘∏’«‘‡§√“–Àåª√‘¡“≥ “√              «—¥°“√¥Ÿ¥°≈◊π· ß UV ¢Õß eurycoma-
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