Page 22 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 16 ฉบับที่ 1

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μ“√“ß∑’Ë 2 º≈°“√»÷°…“§«“¡·¡àπ·≈–§«“¡‡∑’ˬß

Standard %Spike %Recovery HORRAT** ‡°≥±å¬Õ¡√—∫¢Õß %
addition level target Individual Average Recovery*

103.21
Low level 50 105.41 105.08 1.96 95-105
104.55

97.38
Medium level 100 97.63 98.47 1.81 95-105
100.39

98.34
High level 150 94.29 96.14 1.66 95 - 105
95.81

¡‘≈≈‘≈‘μ√ ®—π∑πå≈’≈“ ·≈–‡ªìπμ—«¬“√Õß„πμ”√—∫¬“·°â‰¢âÀâ“√“°

1.8 ¢’¥®”°—¥¢Õß°“√«—¥‡™‘ߪ√‘¡“≥ (Limit ´÷Ë߇ªì𬓠¡ÿπ‰æ√„π∫—≠™’¬“À≈—°·Ààß™“μ‘ ‚¥¬°“√
of Quantitation, LOQ) √—∫ª√–∑“𬓙𑥬“ºß¡—°„Àâ™ß°—∫πÈ”√âÕπ‡æ◊ËÕ„Àâ
LOQ ®“°°“√§”π«≥¡’§à“‡∑à“°—∫ 0.0354 ‰¥âμ—«¬“ÕÕ°¡“ ”À√—∫°“√«‘‡§√“–Àåª√‘¡“≥

¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ eurycomanone À“° °—¥¥â«¬πÈ”®–‰¥âª√‘¡“≥
1.9 §«“¡®”‡æ“– (specificity) eurycomanone πâÕ¬ „π°“√«‘‡§√“–Àå¥â«¬«‘∏’∑’Ë
«‘‡§√“–Àå method blank “√√∫°«π peak æ—≤π“¢÷Èπ®÷ß„™â methanol „π°“√ °—¥μ—«Õ¬à“ß

¢Õß eurycomanone ·≈â«π”¡“«‘‡§√“–Àå¥â«¬ High Performance Liq-
2. °“√«‘‡§√“–Àåª√‘¡“≥ “√ eurycomanone uid Chromatography (HPLC) „π°“√æ—≤π“«‘∏’
„π√“°ª≈“‰À≈‡º◊Õ° «‘‡§√“–Àåª√‘¡“≥ “√ eurycomanone „π√“°ª≈“

º≈°“√«‘‡§√“–Àåª√‘¡“≥ eurycomanone „π ‰À≈‡º◊Õ° ®“°°“√∑¥ Õ∫§«“¡„™â‰¥â¢Õß«‘∏’
√“°ª≈“‰À≈‡º◊Õ° ®”π«π 27 μ—«Õ¬à“ß æ∫«à“ ª√‘¡“≥ «‘‡§√“–Àåª√‘¡“≥ eurycomanone æ∫«à“°“√

eurycomanone ¡’§à“√–À«à“ß 1.1-8.5 % ‚¥¬πÈ”Àπ—° «‘‡§√“–Àå¡’§«“¡·¡à𬔠‚¥¬æ‘®“√≥“®“° %recov-
¡’§à“‡©≈’ˬª√‘¡“≥ eurycomanone ‡∑à“°—∫ 3.7% ery ¢Õß°“√«‘‡§√“–Àå spike sample Õ¬Ÿà„π‡°≥±å
‚¥¬πÈ”Àπ—° ·≈–¡’§à“‡∫’ˬ߇∫π¡“μ√∞“π ‡∑à“°—∫ 1.9 °“√¬Õ¡√—∫ (95-105%) ·≈–§à“ HORRAT Õ¬Ÿà„π

(μ“√“ß∑’Ë 3 ·≈–√Ÿª∑’Ë 8) ‡°≥±å°“√¬Õ¡√—∫ (HORRAT < 2) ´÷Ëß· ¥ß«à“«‘∏’
¥—ß°≈à“«¡’§«“¡‡∑’Ë¬ß ·≈– “¡“√∂𔉪„™â«‘‡§√“–Àå
Õ¿‘ª√“¬º≈
ª√‘¡“≥ eurycomanone „πμ—«Õ¬à“ß√“°ª≈“‰À≈
√“°ª≈“‰À≈‡º◊Õ°„™â‡ªìπ à«πº ¡À≈—°¢Õ߬“ ‡º◊Õ° «‘∏’∑’ˉ¥âæ—≤π“¢÷Èπ¡’§«“¡ –¥«° √«¥‡√Á« „π