Page 100 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 14 ฉบับที่ 3

318

were comparable, as shown in Figure 1. The 3. Comparison of extraction method

method exhibited a good linearity over a con- Flow chart comparing for sample pre-
centration range of 20-140 μg/mL with a cor- paration procedures of reflux and optimized
relation coefficient (r) of 0.9999, as demon- condition of ultrasonication is demonstrated

strated in Figure 2. The recovery and preci- in Figure 3. A total of 30 different lots of
sion results and are summarized in Table 2 Andrographis capsules from 17 manufac-
and 3, respectively. turers were assayed for their andrographolide

contents using both ultrasonication and reflux

Figure 1 Chromatograms of sample solutions obtained from reflux extraction (A) and ultrasonication (B)

Figure 2 Calibration curve of andrographolide