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°“√‡μ√’¬¡μ—«Õ¬à“ß
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ª√‘¡“μ√ 20 ¡‘≈≈‘≈‘μ√ ‰ª °—¥‚¥¬«‘∏’ reflux ‡ªìπ‡«≈“
20 π“∑’ °√Õߺà“π°√–¥“…°√Õß ‡∫Õ√å 4
°“√‡μ√’¬¡ “√≈–≈“¬ “√¡“μ√∞“π
≈–≈“¬ “√ oxyresveratrol πÈ”Àπ—° 2 ¡‘≈≈‘°√—¡
„π‡¡∑“πÕ≈ ª√‘¡“μ√ 5 ¡‘≈≈‘≈‘μ√
√–∫∫‚§√¡“‚∑°√“øï
A B C
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√Ÿª∑’Ë 2 TLC chromatogram ¢Õß·°àπ¡–À“¥
§«“¡Àπ“ 0.25 ¡‘≈≈‘‡¡μ√
1. “√ °—¥ methanol ®“°·°àπ¡–À“¥
«—Ø¿“§‡§≈◊ËÕπ∑’Ë §≈Õ‚√øÕ√å¡ : ‡¡∑“πÕ≈
2. oxyresveratrol
4 : 1
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√–¬–∑“ß°“√‡§≈◊ËÕπ∑’Ë 10 ‡´π쑇¡μ√ A. UV 365 π“‚π‡¡μ√
¢Õß«—Ø¿“§‡§≈◊ËÕπ∑’Ë B. UV 254 π“‚π‡¡μ√
C. Anisaldehyde-H SO
ª√‘¡“μ√ “√≈–≈“¬∑’Ë„™â 5 ‰¡‚§√≈‘μ√ ”À√—∫ 2 4
“√≈–≈“¬μ—«Õ¬à“ß 3
‰¡‚§√≈‘μ√ ”À√—∫ “√
≈–≈“¬ “√¡“μ√∞“π 2. °“√æ—≤π“«‘∏’«‘‡§√“–Àåª√‘¡“≥ oxy-
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°“√μ√«®«—¥ resveratrol „π·°àπ¡–À“¥
1) ¿“¬„μâ· ßÕ—≈μ√“‰«‚Õ‡≈μ∑’˧«“¡¬“« 2.1 °“√‡≈◊Õ°™π‘¥¢Õßμ—«∑”≈–≈“¬„π°“√
§≈◊Ëπ 254 ·≈– 365 π“‚π‡¡μ√ °—¥ º≈¢Õß°“√ °—¥μ—«Õ¬à“ß·°àπ¡–À“¥ 300
2) æàπ¥â«¬πÈ”¬“æàπ Anisaldehyde- ¡‘≈≈‘°√—¡ ¥â«¬μ—«∑”≈–≈“¬Õ‘π∑√’¬å™π‘¥μà“ß Ê ª√‘¡“μ√
H SO reagent 50 ¡‘≈≈‘≈‘μ√ ‡ªìπ‡«≈“ 60 π“∑’ æ∫«à“ ‡¡∑“πÕ≈
2 4
º≈°“√μ√«®‡Õ°≈—°…≥å∑“߇§¡’¥â«¬«‘∏’ TLC „π “¡“√∂ °—¥ oxyresveratrol ‰¥â¡“°∑’Ë ÿ¥‚¥¬¡’
μ—«Õ¬à“ß·°àπ¡–À“¥ ®–æ∫ spot ¢Õß oxyresveratrol impurity ™π‘¥Õ◊ËπªπÕÕ°¡“πâÕ¬ ·≈– “√≈–≈“¬
∑’Ë§à“ R ‡∑à“°—∫ 0.75 „π∑ÿ°μ—«Õ¬à“ß ‚¥¬ TLC chro- μ—«Õ¬à“ß∑’Ë °—¥‰¥â¡’§«“¡§ßμ—«¥’∑’ËÕÿ≥À¿Ÿ¡‘ÀâÕß„π
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matogram ¥—ß· ¥ß„π√Ÿª∑’Ë 2 ·≈–æ∫«à“ ª√‘¡“≥ ¿“™π–°—π· ß ‡ªìπ√–¬–‡«≈“Õ¬à“ßπâÕ¬ 2 «—π ¥—ßπ—Èπ
μË” ÿ¥∑’Ëμ√«®æ∫ (limit of detection; LOD) ¢Õß „π°“√æ—≤π“«‘∏’«‘‡§√“–Àåπ’È®÷߇≈◊Õ°„™â‡¡∑“πÕ≈‡ªìπ
oxyresveratrol ¡’§à“‡∑à“°—∫ 450 π“‚π°√—¡/spot μ—«∑”≈–≈“¬„π°“√ °—¥