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                  ·°àπ¡–À“¥ ‰¥â®“°æ√√≥‰¡â∑’Ë¡’™◊ËÕ«‘∑¬“-  º‘«∫“ß (Thin-Layer chromatography; TLC)
             »“ μ√å«à“ Artocarpus lacucha Buch.-Ham. «ß»å  2) »÷°…“«‘∏’«‘‡§√“–Àåª√‘¡“≥ “√ ”§—≠ oxyresveratrol
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             Moraceae ‡ªìπ‰¡â¬◊πμâπ  Ÿßª√–¡“≥ 30 ‡¡μ√ ∑√ß  „π·°àπ¡–À“¥¥â«¬‡∑§π‘§ Ultra Performance
             μâπ‡ªìπæÿà¡·ºà°«â“ß „∫‡¥’ˬ«‡√’¬ß ≈—∫ √Ÿª¢Õ∫¢π“π  Liquid Chromatography (UPLC) „À≥⫑∏’«‘‡§√“–Àå

             À√◊Õ√Ÿª«ß√’ °«â“ß 8-10 ‡´π쑇¡μ√ ¬“« 10-20  ∑’Ë¡’§«“¡∂Ÿ°μâÕß ·¡à𬔠√«¥‡√Á« ‡™◊ËÕ∂◊Õ‰¥â ·≈–
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             ‡À≈◊Õß º‘«¢√ÿ¢√– ¡’¢ππÿà¡ „π∑“ß°“√·æ∑¬å·ºπ‰∑¬
             „™â‡ª≈◊Õ°μâπ¢—∫欓∏‘‰ â‡¥◊Õπ 欓∏‘‡ âπ¥â“¬ ·≈–  1. μ—«Õ¬à“ß·°àπ¡–À“¥

             欓∏‘μ—«μ◊¥ ·°àπ¡–À“¥¡’ √√æ§ÿ≥·°â°√–…—¬ §ÿ¡     μ—«Õ¬à“ß·°àπ¡–À“¥®“°·À≈àßμà“ß Ê ∑—Èß·À≈àß
             °”‡π‘¥ ·°âª«¥‡ âπ‡ÕÁπ ‡ âπ‡ÕÁπæ‘°“√ ·°âÕ“‡®’¬π  ∏√√¡™“μ‘·≈–√â“π ¡ÿπ‰æ√ ®”π«π 10 μ—«Õ¬à“ß „π
             ≈–≈“¬‡≈◊Õ¥ ·°â≈¡ √–∫“¬∑âÕß ·°âº◊Ëπ§—π ‡π◊ÈÕ‰¡â  √–À«à“߇¥◊Õπ¡‘∂ÿπ“¬π 2556 - °ÿ¡¿“æ—π∏å 2557 π”

             ¡–À“¥ „™â·°âº¥º◊Ëπ§—π ‚√§º‘«Àπ—ß ¢—∫欓∏‘μ—«μ◊¥  ¡“Õ∫·Àâß ∫¥‡ªìπºß≈–‡Õ’¬¥ ºà“π·√à߇∫Õ√å 180
             欓∏‘‰ â‡¥◊Õπ ·°â®ÿ°‡ ’¬¥  “√ °—¥∑’ˉ¥â®“°°“√π”  ‡°Á∫„π¿“™π–·°â« ’™“ªî¥ π‘∑
             ·°àπ¡–À“¥‰ª °—¥¥â«¬πÈ”·≈â«μ—°øÕß∑’Ë≈Õ¬Õ¬Ÿà‡Õ“   2.  “√¡“μ√∞“π
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             ‰ª∑”„Àâ·Àâß ‡√’¬°«à“ ª«°À“¥  “√ ”§—≠∑’Ëæ∫„π     oxyresveratrol §«“¡∫√‘ ÿ∑∏‘Ï  ≥ 97.0 %
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             ·°àπ¡–À“¥ §◊Õ oxyresveratrol ´÷Ëß¡’ƒ∑∏‘Ï∑“ß  (Sigma, Switzerland)
             ™’«¿“æ¡“°¡“¬ ‡™àπ ƒ∑∏‘Ϭ—∫¬—Èß tyrosinase ´÷Ëß  3.  “√‡§¡’·≈–μ—«∑”≈–≈“¬
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             ‡ªìπ‡Õπ‰´¡å„π°√–∫«π°“√ √â“߇¡≈“π‘π ƒ∑∏‘Ïμâ“π    methanol HPLC ·≈– AR (Merck, Ger-
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             ‰°≈‡§™—Ëπ μâ“πÕÕ°´‘‡¥™—Ë𠇪ìπμâπ ªí®®ÿ∫—π ¡’  many), chloroform AR (Merck, Germany), DI
             °“√π”·°àπ¡–À“¥¡“„™âª√–‚¬™πåÕ¬à“ß·æ√àÀ≈“¬„π  Water, anisaldehyde, sulfuric acid, acetic acid

             ∑“߇§√◊ËÕß ”Õ“ß ·°àπ¡–À“¥∑’Ë¡’¢“¬„π∑âÕßμ≈“¥¡’   4. ‡§√◊ËÕß¡◊Õ
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             √“§“·æß¡“° °“√§«∫§ÿ¡§ÿ≥¿“æ®÷ß¡’§«“¡ ”§—≠        Acquity  Ultra Performance Liquid
             ∑”„Àâ “¡“√∂‡≈◊Õ°·°àπ¡–À“¥∑’Ë¡’§ÿ≥¿“楒·≈–¡’  Chromatograph-PDA detector (Waters, USA)

             ª√‘¡“≥ “√ ”§—≠ Ÿß¡“„™â„π°“√º≈‘쬓·≈–       ·≈– Empower 2 software, ‡§√◊ËÕß™—Ë߉øøÑ“ §«“¡
             º≈‘μ¿—≥±å®“° ¡ÿπ‰æ√∑’Ë¡’§ÿ≥¿“楒‰¥â Õ¬à“߉√°Áμ“¡  ≈–‡Õ’¬¥ 0.01 ¡‘≈≈‘°√—¡ (Mettler-Toledo, Swit-
             «‘∏’«‘‡§√“–Àåª√‘¡“≥ oxyresveratrol „π·°àπ¡–À“¥  zerland), UV Cabinet (CAMAG, Switzerland)

             ¬—߉¡à¡’¡“°π—° «‘∏’∑’Ë¡’√“¬ß“πμ’æ‘¡æ出¬·æ√à ‡™àπ  ·≈– TLC plate heater (CAMAG, Switzerland)
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             capillary zone electrophoresis , densitometry   5. «— ¥ÿ«‘∑¬“»“ μ√å·≈–‡§√◊ËÕß·°â«
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             ‡ªìπμâπ °“√»÷°…“π’È®÷ß¡’«—μ∂ÿª√– ß§å‡æ◊ËÕ 1) »÷°…“  Acquity  UPLC BEH C18, 2.1x50 mm,
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