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2.2 °“√»÷°…“√–¬–‡«≈“∑’ˇÀ¡“– ¡„π°“√ ‡§≈◊ËÕπ∑’Ë ∑’ËÕ—μ√“°“√‰À≈ 0.7 ¡‘≈≈‘≈‘μ√/π“∑’ μ—Èß
°—¥ º≈¢Õß°“√ °—¥μ—«Õ¬à“ß·°àπ¡–À“¥ 300 Õÿ≥À¿Ÿ¡‘¢Õßμ—«Õ¬à“ß·≈–§Õ≈—¡πå 40 Õß»“‡´≈‡´’¬

¡‘≈≈‘°√—¡ ¥â«¬‡¡∑“πÕ≈ª√‘¡“μ√ 50 ¡‘≈≈‘≈‘μ√ ‚¥¬ ®“°º≈°“√»÷°…“¢â“ßμâπ “¡“√∂ √ÿª«‘∏’
«‘∏’ reflux ∑’ˇ«≈“μà“ß Ê μ—Èß·μà 30-120 π“∑’ æ∫«à“ «‘‡§√“–Àåª√‘¡“≥ oxyresveratrol ‰¥â¥—ßπ’È
√–¬–‡«≈“ °—¥ 60-120 π“∑’ “¡“√∂ °—¥ °“√‡μ√’¬¡μ—«Õ¬à“ß

oxyresveratrol ÕÕ°¡“‰¥â‡∑à“°—π · ¥ß«à“ °“√ °—¥ π”ºß·°àπ¡–À“¥ πÈ”Àπ—° 300 ¡‘≈≈‘°√—¡ ¡“
oxyresveratrol “¡“√∂‡°‘¥¢÷Èπ‰¥âÕ¬à“ß ¡∫Ÿ√≥å∑’Ë °—¥¥â«¬‡¡∑“πÕ≈ ª√‘¡“μ√ 50 ¡‘≈≈‘≈‘μ√ °√Õß
‡«≈“ 60 π“∑’ ¥—ßπ—Èπ „π°“√æ—≤π“«‘∏’«‘‡§√“–Àåπ’È®÷ß ·≈â«√–‡À¬·Àâß ®“°π—Èπ≈–≈“¬°≈—∫·≈–ª√—∫

‡≈◊Õ°„™â√–¬–‡«≈“ ”À√—∫°“√ °—¥ 60 π“∑’ ª√‘¡“μ√¥â«¬ 50 % ‡¡∑“πÕ≈ ®π§√∫ 100 ¡‘≈≈‘≈‘μ√
2.3 °“√»÷°…“√–∫∫‚§√¡“‚∑°√“øï §«“¡ √–∫∫‚§√¡“‚∑°√“øï
¬“«§≈◊Ëπ„π°“√μ√«®«—¥ oxyresveratrol æ∫«à“ π” “√≈–≈“¬μ—«Õ¬à“ß∑’ˉ¥â ª√‘¡“μ√ 3

“√¥—ß°≈à“«¡’§à“°“√¥Ÿ¥°≈◊π· ß Ÿß ÿ¥‡∑à“°—∫ 254, ‰¡‚§√≈‘μ√ ©’¥‡¢â“ Ÿà√–∫∫‚§√¡“‚∑°√“øï ´÷Ëß
286 ·≈– 326 π“‚π‡¡μ√ ·μà‡¡◊ËÕ∑¥ Õ∫°—∫ ª√–°Õ∫¥â«¬ §Õ≈—¡πå™π‘¥ BEH C18, 2.1×50 mm,
μ—«Õ¬à“ß·°àπ¡–À“¥ æ∫«à“ ∑’˧«“¡¬“«§≈◊Ëπ 326 1.7 μm ·≈– 20 % ‡¡∑“πÕ≈ ‡ªìπ«—Ø¿“§‡§≈◊ËÕπ∑’Ë

π“‚π‡¡μ√ ‰¡àæ∫ impurity Õ◊Ëπ®“°·°àπ¡–À“¥∑’Ë Õ—μ√“°“√‰À≈ 0.7 ¡‘≈≈‘≈‘μ√/π“∑’ Õÿ≥À¿Ÿ¡‘¢Õß
¥Ÿ¥°≈◊π· ßÕ—≈μ√“‰«‚Õ‡≈μ∑’˧«“¡¬“«§≈◊Ëππ’È μ—«Õ¬à“ß·≈–§Õ≈—¡πå∑’Ë 40 Õß»“‡´≈‡´’¬ ·≈–μ√«®
«—Ø¿“§§ß∑’Ë °“√‡ª√’¬∫‡∑’¬∫º≈¢ÕߧÕ≈—¡πå «—¥ “√¥â«¬· ßÕ—≈μ√“‰«‚Õ‡≈μ∑’˧«“¡¬“«§≈◊Ëπ 326
™π‘¥μà“ß Ê ∑’Ë·μ°μà“ß°—π„π‡√◊ËÕߢÕß™π‘¥Õπÿ¿“§ π“‚π‡¡μ√ ‚¥¬ peak ¢Õß oxyresveratrol ®–

¿“¬„π (BEH C18, BEH C8 ·≈– BEH Phenyl) ª√“°Ø∑’Ë retention time ‡∑à“°—∫ 1.7 π“∑’ chro-
§«“¡¬“«¢ÕߧÕ≈—¡πå (50 ·≈– 100 ¡‘≈≈‘‡¡μ√) matogram ¢Õß “√≈–≈“¬μ—«Õ¬à“ß·°àπ¡–À“¥

‚¥¬æ‘®“√≥“®“°≈—°…≥– peak ¢Õß oxyresveratrol ¥—ß· ¥ß„π√Ÿª∑’Ë 3
(peak shape ·≈– peak symmetry) ·≈– reten- 2.4 °“√∑¥ Õ∫§«“¡„™â‰¥â¢Õß«‘∏’ æ∫«à“
tion time æ∫«à“ §Õ≈—¡πå™π‘¥ BEH C18, 2.1×50 calibration curve ¢Õß “√ oxyresveratrol ¡’
mm, 1.7 μm „Àâ peak ¢Õß oxyresveratrol ¥’∑’Ë ÿ¥ §«“¡‡ªìπ‡ âπμ√ß„π™à«ß 0.06-0.42 ¡‘≈≈‘°√—¡/
2
∑’Ë retention time 1.7 π“∑’ ¡‘≈≈‘≈‘μ√ ¡’§à“ —¡ª√– ‘∑∏‘ϧ«“¡ —¡æ—π∏å (R ) ‡∑à“°—∫
«—Ø¿“§‡§≈◊ËÕπ∑’Ë º≈°“√»÷°…“™π‘¥·≈– —¥ 0.9998 §à“√âÕ¬≈–¢Õß “√¥—ß°≈à“«∑’ˉ¥â§◊π°≈—∫¡“ (%

à«π¢Õß«—Ø¿“§‡§≈◊ËÕπ∑’Ë∑’Ë®–„™â‚¥¬æ‘®“√≥“®“° recovery) Õ¬Ÿà„π™à«ß 95.75-100.36 % ¡’§à“ª√‘¡“≥
peak shape ·≈– retention time ¢Õß oxy- μË” ÿ¥∑’Ëμ√«®æ∫ (LOD) ·≈–ª√‘¡“≥μË” ÿ¥∑’Ë
resveratrol §«“¡ “¡“√∂„π°“√·¬° impurity “¡“√∂√“¬ß“π§à“‡ªìπμ—«‡≈¢‰¥â (LOQ) ‡∑à“°—∫ 0.005
Õ◊ËπÕÕ°®“° oxyresveratrol „πμ—«Õ¬à“ß·°àπ¡–À“¥ ·≈– 0.017 ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ μ“¡≈”¥—∫

·≈–§«“¡¥—π¢Õß√–∫∫ ¥—ßπ—Èπ „π°“√æ—≤π“«‘∏’ 3. °“√»÷°…“§ÿ≥¿“æ¢Õß·°àπ¡–À“¥
«‘‡§√“–Àåπ’È ®÷߇≈◊Õ°„™â 20 % ‡¡∑“πÕ≈ ‡ªìπ«—Ø¿“§ ®“°°“√«‘‡§√“–Àåª√‘¡“≥ oxyresveratrol „π