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Journal of Thai Traditional & Alternative Medicine  Vol. 12 No. 1 January-April 2014  37



               ≈–≈“¬¥â«¬ acetic anhydride ·≈⫧àÕ¬ Ê ‡μ‘¡        (2) °“√‡μ√’¬¡ “√≈–≈“¬¡“μ√∞“π
               °√¥°”¡–∂—π‡¢â¡¢âπ 1.0 ¡‘≈≈‘≈‘μ√  —߇°μº≈∑’ˇ°‘¥¢÷Èπ  ≈–≈“¬ “√ 3-(3,4-dihydroxy phenyl) lactic acid,

               (μ“√“ß∑’Ë 1)                              rosmarinic acid, caffeic acid „Àâ¡’§«“¡‡¢â¡¢âπ
                      (2) Iron (III) Chloride Test Solution:  1.0 ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ „π‡¡∑“πÕ≈
               ™—Ëßμ—«Õ¬à“ß 0.1 °√—¡ ‡μ‘¡πÈ”°≈—Ëπ 20 ¡‘≈≈‘≈‘μ√ π”  (3) πÈ”¬“·¬° ‡μ√’¬¡πÈ”¬“·¬°∑’Ëæ—≤π“

               ‰ª„ à„πÕà“߇ ’¬ß§«“¡∂÷Ë Ÿß ·≈â« sonicate π“π 30  ‡Õß‚¥¬º ¡§≈Õ‚√øÕ√å¡ ‡¡∑“πÕ≈·≈–°√¥øÕ√å¡‘°
               π“∑’ °√Õß π” “√≈–≈“¬∑’Ë°√Õ߉¥â 2 ¡‘≈≈‘≈‘μ√ „ à  „πÕ—μ√“ à«π 40: 10: 1 „À⇢⓰—π¥’ π”¡“„ à„π∂—ß
               „πÀ≈Õ¥∑¥≈Õß ·≈â«‡μ‘¡ “√≈–≈“¬ ferric chloride  ‚§√¡“‚∑°√“øï ∑‘È߉«âÕ¬à“ßπâÕ¬ 1 ™—Ë«‚¡ß°àÕπ„™â

               2-3 À¬¥  —߇°μº≈∑’ˇ°‘¥¢÷Èπ (μ“√“ß∑’Ë 1)  ‡æ◊ËÕ„Àâ∫√√¬“°“»„π∂—ßÕ‘Ë¡μ—«¥â«¬πÈ”¬“·¬°
                   2. °“√æ‘ Ÿ®πå‡Õ°≈—°…≥å∑“߇§¡’¥â«¬«‘∏’         (4) «‘∏’°“√ „™âÀ≈Õ¥√Ÿ‡≈Á° (capillary tube)
               ‚§√¡“‚∑°√“øﺑ«∫“ß (Thin-layer Chromatogra-  ∫√√®ÿ “√≈–≈“¬μ—«Õ¬à“ß 5 ‰¡‚§√≈‘μ√ ·≈–
                  48
               phy)                                       “√≈–≈“¬¡“μ√∞“π 2 ‰¡‚§√≈‘μ√ ¡“·μâ¡∫π·ºàπ
                      (1) °“√‡μ√’¬¡ “√≈–≈“¬μ—«Õ¬à“ß ™—Ëß  ‡§≈◊Õ∫´‘≈‘°“‡®≈„π·π«√–¥—∫‡¥’¬«°—π ‚¥¬„ÀâÀà“ß
               μ—«Õ¬à“ß 500 ¡‘≈≈‘°√—¡ ≈–≈“¬„π 50% ‡¡∑“πÕ≈  ®“°¢Õ∫≈à“ߪ√–¡“≥ 2 ‡´π쑇¡μ√ ·≈–„Àâ¡’√–¬–

               10 ¡‘≈≈‘≈‘μ√                              Àà“ß√–À«à“ß “√≈–≈“¬·μà≈–™π‘¥‰¡àπâÕ¬°«à“ 1



               μ“√“ß∑’Ë 2 §à“ hR ·≈–º≈°“√μ√«® Õ∫ “√ª√–°Õ∫∑“߇§¡’„π “√ °—¥¥â«¬ 50% ‡¡∑“πÕ≈®“° “√ °—¥·¡ß≈—°§“
                            f
                                                                  °“√μ√«® Õ∫¥â«¬ anisaldehyde TS
                ®ÿ¥ ’                       §à“ hRf
                                                                 ·≈– —߇°μº≈¥â«¬· ß∏√√¡™“μ‘ ( ’)
                 1                          5  -  7                          πÈ”μ“≈
                 2                         13 - 18                         πÈ”μ“≈ÕàÕπ
                 3*                        21 - 25                           ™¡æŸ
                 4**                       31 - 35                           ™¡æŸ
                 5***                      48 - 51                           πÈ”μ“≈
                 6                         55 - 56                          ¡à«ßÕàÕπ
                 7                         56 - 57                          ¡à«ßÕàÕπ
                 8                         59 - 60                          ¡à«ßÕàÕπ
                 9                         62 - 65                          ¡à«ßÕàÕπ
                10                         70 - 75                          ¡à«ßÕàÕπ
                11                         95 - 97                            ¡à«ß
                12                         98 - 99                            ¡à«ß
               À¡“¬‡Àμÿ * 3-(3,4-dihydroxy phenyl)-3-hydroxypropanoic acid
                       ** rosmarinic acid
                       ***caffeic acid
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