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Journal of Thai Traditional & Alternative Medicine Vol. 12 No. 1 January-April 2014 37

≈–≈“¬¥â«¬ acetic anhydride ·≈⫧àÕ¬ Ê ‡μ‘¡ (2) °“√‡μ√’¬¡ “√≈–≈“¬¡“μ√∞“π
°√¥°”¡–∂—π‡¢â¡¢âπ 1.0 ¡‘≈≈‘≈‘μ√ —߇°μº≈∑’ˇ°‘¥¢÷Èπ ≈–≈“¬ “√ 3-(3,4-dihydroxy phenyl) lactic acid,

(μ“√“ß∑’Ë 1) rosmarinic acid, caffeic acid „Àâ¡’§«“¡‡¢â¡¢âπ
(2) Iron (III) Chloride Test Solution: 1.0 ¡‘≈≈‘°√—¡/¡‘≈≈‘≈‘μ√ „π‡¡∑“πÕ≈
™—Ëßμ—«Õ¬à“ß 0.1 °√—¡ ‡μ‘¡πÈ”°≈—Ëπ 20 ¡‘≈≈‘≈‘μ√ π” (3) πÈ”¬“·¬° ‡μ√’¬¡πÈ”¬“·¬°∑’Ëæ—≤π“

‰ª„ à„πÕà“߇ ’¬ß§«“¡∂÷Ë Ÿß ·≈â« sonicate π“π 30 ‡Õß‚¥¬º ¡§≈Õ‚√øÕ√å¡ ‡¡∑“πÕ≈·≈–°√¥øÕ√å¡‘°
π“∑’ °√Õß π” “√≈–≈“¬∑’Ë°√Õ߉¥â 2 ¡‘≈≈‘≈‘μ√ „ à „πÕ—μ√“ à«π 40: 10: 1 „À⇢⓰—π¥’ π”¡“„ à„π∂—ß
„πÀ≈Õ¥∑¥≈Õß ·≈â«‡μ‘¡ “√≈–≈“¬ ferric chloride ‚§√¡“‚∑°√“øï ∑‘È߉«âÕ¬à“ßπâÕ¬ 1 ™—Ë«‚¡ß°àÕπ„™â

2-3 À¬¥ —߇°μº≈∑’ˇ°‘¥¢÷Èπ (μ“√“ß∑’Ë 1) ‡æ◊ËÕ„Àâ∫√√¬“°“»„π∂—ßÕ‘Ë¡μ—«¥â«¬πÈ”¬“·¬°
2. °“√æ‘ Ÿ®πå‡Õ°≈—°…≥å∑“߇§¡’¥â«¬«‘∏’ (4) «‘∏’°“√ „™âÀ≈Õ¥√Ÿ‡≈Á° (capillary tube)
‚§√¡“‚∑°√“øﺑ«∫“ß (Thin-layer Chromatogra- ∫√√®ÿ “√≈–≈“¬μ—«Õ¬à“ß 5 ‰¡‚§√≈‘μ√ ·≈–
48
phy) “√≈–≈“¬¡“μ√∞“π 2 ‰¡‚§√≈‘μ√ ¡“·μâ¡∫π·ºàπ
(1) °“√‡μ√’¬¡ “√≈–≈“¬μ—«Õ¬à“ß ™—Ëß ‡§≈◊Õ∫´‘≈‘°“‡®≈„π·π«√–¥—∫‡¥’¬«°—π ‚¥¬„ÀâÀà“ß
μ—«Õ¬à“ß 500 ¡‘≈≈‘°√—¡ ≈–≈“¬„π 50% ‡¡∑“πÕ≈ ®“°¢Õ∫≈à“ߪ√–¡“≥ 2 ‡´π쑇¡μ√ ·≈–„Àâ¡’√–¬–

10 ¡‘≈≈‘≈‘μ√ Àà“ß√–À«à“ß “√≈–≈“¬·μà≈–™π‘¥‰¡àπâÕ¬°«à“ 1

μ“√“ß∑’Ë 2 §à“ hR ·≈–º≈°“√μ√«® Õ∫ “√ª√–°Õ∫∑“߇§¡’„π “√ °—¥¥â«¬ 50% ‡¡∑“πÕ≈®“° “√ °—¥·¡ß≈—°§“
f
°“√μ√«® Õ∫¥â«¬ anisaldehyde TS
®ÿ¥ ’ §à“ hRf
·≈– —߇°μº≈¥â«¬· ß∏√√¡™“μ‘ ( ’)
1 5 - 7 πÈ”μ“≈
2 13 - 18 πÈ”μ“≈ÕàÕπ
3* 21 - 25 ™¡æŸ
4** 31 - 35 ™¡æŸ
5*** 48 - 51 πÈ”μ“≈
6 55 - 56 ¡à«ßÕàÕπ
7 56 - 57 ¡à«ßÕàÕπ
8 59 - 60 ¡à«ßÕàÕπ
9 62 - 65 ¡à«ßÕàÕπ
10 70 - 75 ¡à«ßÕàÕπ
11 95 - 97 ¡à«ß
12 98 - 99 ¡à«ß
À¡“¬‡Àμÿ * 3-(3,4-dihydroxy phenyl)-3-hydroxypropanoic acid
** rosmarinic acid
***caffeic acid