Page 15 - วารสารกรมการแพทย์แผนไทยฯ ปีที่ 11 ฉบับที่ 1

Journal of Thai Traditional & Alternative Medicine Vol. 11 No. 1 January-April 2013 9

√â“ßÕπÿ¡Ÿ≈Õ‘ √–´ÿª‡ªÕ√åÕÕ°‰´¥å·Õπ‰ÕÕÕπ (su- ∑¥≈Õߥ⫬«‘∏’°“√μà“ß Ê æ∫«à“ “√ 5,7-DMF

peroxide anion) ‡æ‘Ë¡¢÷Èπ∂â“‡μ‘¡ “√ °—¥°√–™“¬ “¡“√∂ μâ“π°“√Õ—°‡ ∫·∫∫‡©’¬∫æ≈—π‰¥â¥’°«à“
¥”¥â«¬‡Õ∑“πÕ≈≈ß„π™‘Èπ à«π∑àÕ‡≈◊Õ¥·¥ß®–≈¥ ·∫∫‡√◊ÈÕ√—ß ‚¥¬· ¥ßƒ∑∏‘Ϭ—∫¬—Èß°“√∫«¡¢ÕßÕÿâ߇∑â“
°“√ √â“ß´ÿª‡ªÕ√åÕÕ°‰´¥å·Õπ‰ÕÕÕπ≈ß ·≈–‡æ‘Ë¡ ÀπŸ¢“«®“° “√§“√“®’π·ππ·≈–‡§‚Õ≈‘π‰¥â¥’°«à“ƒ∑∏‘Ï

ƒ∑∏‘Ï¢Õß acetylcholine „π°“√∑”„Àâ∑àÕ‡≈◊Õ¥·¥ß ¬—∫¬—Èß°“√ √â“ß·°√πŸ‚≈¡“ (granuloma) ®“°°“√
§≈“¬μ—« ·≈–‡¡◊ËÕ„ÀâÀπŸ·√μ∑’ˇªìπ‡∫“À«“π°‘π “√ Ωíß ”≈’„μ⺑«Àπ—ß πÕ°®“°π’È æ∫«à“ “√ 5,7-DMF
°—¥¢π“¥ 100 ¡°./πÈ”Àπ—°μ—« 1 °°. π“π 4 —ª¥“Àå ¡’ƒ∑∏‘Ϭ—∫¬—Èß°“√ √â“ß exudate ·≈–°“√ √â“ß “√

æ∫«à“∑”„Àâ∑àÕ‡≈◊Õ¥·¥ß≈¥°“√ √â“ß´ÿª‡ªÕ√åÕÕ°‰´¥å æ√Õ μ“·°≈π¥‘πÕ¬à“ß¡’π—¬ ”§—≠ ‡¡◊ËÕ»÷°…“ƒ∑∏‘Ï
·Õπ‰ÕÕÕπ ‡æ‘Ë¡°“√ √â“߉πμ√‘°ÕÕ°‰´¥å·≈–∑”„Àâ μâ“π°“√Õ—°‡ ∫„π™àÕߪե¢ÕßÀπŸ¢“« (rat pleu-
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∑àÕ‡≈◊Õ¥·¥ß§≈“¬μ—« risy)
4. º≈μàÕ‡¡·∑∫Õ≈‘´÷¡¢Õ߉¢¡—π √“¬ß“π°“√»÷°…“ƒ∑∏‘Ïμâ“πÕ—°‡ ∫¢Õß°√–™“¬
π—°«‘®—¬¢Õß≠’˪ÿÉπæ∫«à“ “√ °—¥‡Àßâ“°√–™“¬¥” ¥”¢Õߧ≥–‡¿ —™»“ μ√å ¡À“«‘∑¬“≈—π ߢ≈“π§√‘π∑√å

¥â«¬‡Õ∑‘≈·Õ´‘‡∑μ “¡“√∂≈¥§«“¡º‘¥ª√°μ‘¢Õß æ∫«à“ “√ 5-hydroxy-3,7,3',4'-tetrametho-
‡¡·∑∫Õ≈‘´÷¡„πÀπŸ∂’∫®—°√∑’ËÕâ«π ·≈–¡’º≈≈¥°“√ xyflavone ∑’Ë·¬°‰¥â®“° “√ °—¥°√–™“¬¥”¥â«¬

– ¡¢Õ߉¢¡—π‰¥â ‡¡◊ËÕ»÷°…“°≈‰°°“√ÕÕ°ƒ∑∏‘Ï„π ‡Õ∑“πÕ≈ ¡’ƒ∑∏‘Ï Ÿß ÿ¥„π°“√¬—∫¬—Èß°“√À≈—Ë߉πμ√‘°-
‡´≈≈剢¡—π (adipocyte) æ∫«à“ “√ °—¥¥—ß°≈à“« ÕÕ°‰´¥å®“° RAW264.7 macrophage cells
·≈– “√ 3,5,7,4û-tetramethoxyflavone ·≈– 3,5,7, ‡¡◊ËÕ°√–μÿâπ¥â«¬ lipopolysaccharide (LPS) ‚¥¬¡’

3û,4û-pentamethoxy-flavone ¡’ƒ∑∏‘Ï·√ß„π°“√ §à“ IC = 16.1 ‰¡‚§√‚¡≈“√å √«¡∑—È߬—∫¬—Èß°“√À≈—Ëß
50
°√–μÿâπ„À⇴≈≈å 3T3-L1 preadipocytes ‡ª≈’ˬπ PGE2 (IC = 16.3 ‰¡‚§√‚¡≈“√å) ·μà‰¡à¡’º≈μàÕ
50
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¿“æ (differentiate) ‰ª‡ªìπ‡´≈≈å adipocytes °“√ √â“ß tumor necrosis factor ((TNFα) à«π
·≈– “√∑—Èß Õß™π‘¥°√–μÿâπ„Àâ¡’°“√‡æ‘Ë¡√–¥—∫ “√ °—¥°√–™“¬¥”¥â«¬‡Õ∑“πÕ≈¡’ƒ∑∏‘Ϭ—∫¬—Èß°“√À≈—Ëß
adiponectin mRNA ·≈–°“√À≈—Ëß adiponectin PGE2 ‚¥¬¡’ IC = 9.2 ¡§°./¡≈.) “√ °—¥π’È·≈–
50
≈ß„π¡’‡¥’¬ √«¡∑—È߇æ‘Ë¡°“√· ¥ßÕÕ°¢Õß peroxi- “√ 5-hydroxy-3,7,3',4'-tetramethoxyflavone

some proliferator-activated receptor γ (PPAR γ) ¬—∫¬—Èß°“√· ¥ßÕÕ°¢Õß inducible nitric oxide
´÷Ë߇ªìπ nuclear receptor ∑’ˇ¡◊ËÕ°√–μÿâπ®–‡æ‘Ë¡°“√ synthase (iNOS) mRNA Õ¬à“ß¡’§«“¡ —¡æ—π∏å
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¥÷߉¢¡—π‡¢â“‡´≈≈å·≈–°“√ √â“߉¢¡—π‚¥¬‡´≈≈剢¡—π °—∫§«“¡‡¢â¡¢âπ∑’Ë„™â ¢≥–∑’Ë¡’º≈μàÕ°“√· ¥ßÕÕ°
5. ƒ∑∏‘Ïμâ“πÕ—°‡ ∫ ¢Õß cyclooxygenase-2 (COX-2) mRNA ‡æ’¬ß
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“√ 5,7-dimethoxyflavone (5,7-DMF) ∑’Ë ∫“ß à«π
·¬°‰¥â®“°‡Àßâ“°√–™“¬¥” ¡’ƒ∑∏‘Ïμâ“π°“√Õ—°‡ ∫ πÕ°®“°π’È ¬—ßæ∫«à“ “√ °—¥°√–™“¬¥”¥â«¬
‡∑’¬∫‰¥â°—∫¬“¡“μ√∞“πÀ≈“¬™π‘¥ §◊Õ ·Õ ‰æ√‘π, §≈Õ‚√øÕ√å¡· ¥ßƒ∑∏‘Ïμâ“πÕ—°‡ ∫‚¥¬≈¥°“√∫«¡

Õ‘π‚¥‡¡∑“´‘π, ‰Œ‚¥√§Õ√å∑‘‚´π ·≈–‡æ√¥π‘‚´‚≈π ¢ÕßÕÿâ߇∑â“ÀπŸ·√μ‡¡◊ËÕ·¬° “√ ”§—≠ÕÕ°¡“ æ∫«à“
®“°°“√»÷°…“ƒ∑∏‘Ïμâ“πÕ—°‡ ∫¢Õß “√π’È„π —μ«å 5,7-dimethoxyflavone, trimethylapigenin ·≈–